Nano-crystal cellulose fluorescent probe for detecting metal ions and preparation method thereof

A nanocrystalline cellulose and fluorescent probe technology, applied in the intersection of nanomaterials and fluorescent sensing, to achieve the effects of improving selectivity and sensitivity, enhancing complexing ability, and easy regulation of fluorescent signals

Inactive Publication Date: 2018-09-21
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on loaded fluorescent probes based on nanocrystalline cellulose is still in its infancy.

Method used

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  • Nano-crystal cellulose fluorescent probe for detecting metal ions and preparation method thereof
  • Nano-crystal cellulose fluorescent probe for detecting metal ions and preparation method thereof
  • Nano-crystal cellulose fluorescent probe for detecting metal ions and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take 10g of bleached coniferous pulp and soak it in water, use a deflagger to decompose and disperse it, then add TEMPO (0.16g), NaBr (1.6g) and NaClO (110mL), and use NaOH (2mol / L) to adjust the pH between 10 and 10.5 , stirred and reacted for 4h, then suction-filtered, soaked the slurry obtained by suction-filtration twice with HCl (1000mL, 0.1mol / L) solution, and washed until neutral. The obtained sample was decomposed in a decomposer for 5 minutes, the pH value was adjusted to 10-10.5 with NaOH (2 mol / L), and a clear and transparent solution was obtained by ultrasonication. The above solution was freeze-dried for 3 days to obtain nanofibrillar cellulose (NFC).

[0035] Add freeze-dried NFC (3.77g) and HCl (200ml, 3mol / L) into a 500mL four-neck flask, heat to reflux for 3h, cool to room temperature after the reaction, and wash with high-speed centrifugation (10000rpm, 10min) until neutral to obtain nanocrystals Cellulose (NCC), freeze-dried to obtain NCC solid.

[...

Embodiment 2

[0039] Take 10g of bleached coniferous pulp and soak it in water, use a deflagger to decompose and disperse it, then add TEMPO (0.16g), NaBr (1.6g) and NaClO (110mL), and use NaOH (2mol / L) to adjust the pH between 10 and 10.5 , stirred and reacted for 4h, then suction-filtered, soaked the slurry obtained by suction-filtration twice with HCl (1000mL, 0.1mol / L) solution, and washed until neutral. The obtained sample was decomposed in a decomposer for 5 minutes, the pH value was adjusted to 10-10.5 with NaOH (2 mol / L), and a clear and transparent solution was obtained by ultrasonication. The above solution was freeze-dried for 3 days to obtain nanofibrillar cellulose (NFC).

[0040] Add freeze-dried NFC (3.77g) and HCl (200ml, 3mol / L) into a 500ml four-neck flask, heat to reflux for 3h, cool to room temperature after the reaction, and wash with high-speed centrifugation (10000rpm, 10min) until neutral to obtain nanocrystals Cellulose (NCC), freeze-dried to obtain NCC solid.

[...

Embodiment 3

[0044] Bleached softwood pulp was pulverized in a Wiley pulverizer and passed through a 20-mesh sieve, and the obtained cellulose powder (50.0 g) was added to 500 mL of 64 wt% H 2 SO 4 , heated to 45°C under stirring for 45 minutes, then added 10 times deionized water to dilute to terminate the reaction. The mixture was centrifuged at 5000rpm for 10min, and the supernatant was removed. The resulting precipitate was redispersed in deionized water and dialyzed with a regenerated cellulose membrane with a molecular weight cut-off of 12000-14000 for 7 days until the pH value remained unchanged. The dialyzed mixed solution was sonicated in an ice-water bath for 30 minutes, centrifuged at 5000 rpm for 5 minutes, and the slightly turbid nanocrystalline cellulose (NCC) dispersion in the upper layer was collected. Repeat the above process until the supernatant liquid becomes clear after centrifugation, and the obtained nanocrystalline cellulose (NCC) is divided into water dispersion l...

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Abstract

The invention relates to a nano-crystal cellulose fluorescent probe for detecting metal ions and a preparation method thereof, and belongs to the technical field of nanometer materials and fluorescence sensing crossing. The method comprises the steps that firstly, an active functional group is introduced into the surface of nano-crystal cellulose, the nano-crystal cellulose fluorescent probe is constructed through covalent bonding fluorescent dye, by means of the synergic complexing action of the surface active functional group, the fluorescence emission mechanism of the fluorescent dye is changed, and selectivity and sensitivity detection for metal ions are achieved. The nano-crystal cellulose fluorescent probe is an environment-friendly green material and is good in hydrophilia, safe, free of toxin, capable of being completely degraded and capable of being applied to the fields of metal ion sensing detection, fluorescence tracking, bioimaging and the like.

Description

technical field [0001] The invention belongs to the cross-technical field of nanomaterials and fluorescent sensing, and mainly relates to a nanocrystalline cellulose fluorescent probe for detecting metal ions and a preparation method thereof. Background technique [0002] Fluorescent probe technology has the characteristics of high sensitivity, good selectivity, short response time, and non-complex sampling. It has broad application prospects (Wolfbeis OS.Chemical Society Reviews.2015,44(14):4743-68; Zhou Jia, Yang Meipan, Meng Wenfei, et al. Organic Chemistry.2014; 34(8):1646-51; Zhang L, Wang E. Nano Today. 2014, 9(1):132-57). Loaded nano-fluorescent probe is a new type of fluorescent functional material with unique photophysical and chemical properties, which uses inorganic or organic polymers as carriers and organic dye molecules, quantum dots, metal nanoparticles and metal complexes as loads. Compared with molecular probes, it has obvious advantages in biocompatibilit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C08B15/04G01N21/64
CPCC09K11/06C08B15/04C09K2211/145C09K2211/1466G01N21/643
Inventor 吴伟兵宋如愿左克曼戴红旗
Owner NANJING FORESTRY UNIV
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