Surface plasmon resonance and bio-sensing-based water chip

A surface plasmon and biosensing technology, which is used in the measurement of phase influence characteristics, material analysis by optical means, instruments, etc., can solve the problem of inability to quickly test various impurity concentrations, etc., and achieves convenient on-site real-time detection, high sensitivity , the effect of simplifying the optical path and mechanical structure

Inactive Publication Date: 2010-12-29
ZHEJIANG UNIV
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  • Abstract
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Problems solved by technology

[0006] Aiming at the problem that the concentration of various impurities in the water body cannot be quickly tested in the detection of the water body, the present invention provides a water chip based on surface plasmon resonance and biosensing, which can simultaneously detect The concentration of various impurities in the water body

Method used

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  • Surface plasmon resonance and bio-sensing-based water chip
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  • Surface plasmon resonance and bio-sensing-based water chip

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Embodiment 1

[0026] Such as figure 1 As shown, the present invention is a water chip based on surface plasmon resonance and biosensing, including a light emitting component, a light reflecting component, a light polarization modulation component, and a light receiving component;

[0027] The light emitting component is composed of a light source 1 and a beam expander 2. The light source 1 uses a red light source with a wavelength of 740nm, and the light emitted by the light source 1 becomes parallel light after passing through the beam expander;

[0028] The light reflection assembly includes an isosceles rectangular prism 4 and a gold film 5 on its hypotenuse surface, the reflective surface of the gold film 5 faces the isosceles rectangular prism 4, and the thickness of the gold film 5 is about 50 nm;

[0029] The optical polarization state modulation component includes a polarizer 3 located between the beam expander 2 and the incident rectangular surface 14 of the isosceles rectangular p...

Embodiment 2

[0039] The structure of this embodiment is the same as that of Embodiment 1, and the composition of the biosensitive membrane in this embodiment is different from that of Embodiment 1. The concentrated sulfuric acid of 98% (mass percentage) and the hydrogen peroxide of 30% (mass percentage) are configured into mixed solution according to the volume ratio of concentrated sulfuric acid: hydrogen peroxide=7: 3, the gold film 5 on the hypotenuse surface of isosceles rectangular prism 4 Soak in the mixed solution for 12 hours to remove impurities on the surface of the gold film 5. Get the 11-mercaptoundecanoic acid solution of 10mmol / L and drop it on the surface of gold film 5, react for 3 minutes, make 11-mercaptoundecanoic acid form a firm Au -S covalent bond, forming a self-assembled monolayer on the surface of the gold film as a matrix layer. Take 50uL of a mixed solution of N-hydroxysuccinimide (NHS) and dichloroethane (EDC) and drop it on the matrix layer, the concentration ...

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Abstract

The invention discloses a surface plasmon resonance and bio-sensing-based water chip. A light source, a beam expanding lens, a polarizer, an isosceles right-angle prism, a quarter wave plate, a polarization analyzer, an imaging lens and a planar array CCD are arranged in turn along the light direction, wherein the hypotenuse surface of the isosceles right-angle prism is plated with a gold film; biological modification is performed on the gold film so as to form a bio-sensitive film subjected to specific adsorption with impurities in aqueous solution to be detected; the refractive index change of the impurities to the aqueous solution to be detected is converted into light intensity change of reflected light; and the refractive index change is detected by using the SPR effect on the interface between the gold film and the isosceles right-angle prism so as to detect the concentration of substances in the solution. When the water chip is used, the concentration of various substances in the water can be detected at one time, a light path and a mechanical structure are simplified and the water detection efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of industrial testing, in particular to a water chip based on surface plasmon resonance and biosensing. Background technique [0002] Surface Plasmon resonance (SPR) technology is a high-precision detection technology. The basic principle of SPR is that the incident p light undergoes total internal reflection at the interface between the prism and the metal film, and the evanescent wave generated will cause the free electrons on the surface of the metal film to generate surface plasmons. When the frequency and wave number of the evanescent wave and the surface plasmon wave are equal The two will resonate. When the resonance occurs, the energy of the incident light drops sharply, and the minimum light intensity value appears on the reflection spectrum. The conditions for SPR resonance to occur are related to the refractive index of the medium on the other side of the metal film. The refractive index of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/41
CPCG01N21/553
Inventor 王晓萍孙博书黄子昊刘旭
Owner ZHEJIANG UNIV
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