Preparation method of magnetic molecularly imprinted polymer for separating/purifying sudan I

A magnetic molecular imprinting and Sudan red technology, which is applied in chemical instruments and methods, and other chemical processes, can solve the problems of low selectivity and easy damage to the spatial structure of polymers, and achieve the effect of rapid detection and analysis

Inactive Publication Date: 2012-05-02
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a preparation method for separating / purifying Sudan I magnetic molecularly imprinted polymers, which solves the problems that the traditional method needs to be ground and sieved, easily destroys the spatial structure of the polymer, and has low selectivity.

Method used

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  • Preparation method of magnetic molecularly imprinted polymer for separating/purifying sudan I
  • Preparation method of magnetic molecularly imprinted polymer for separating/purifying sudan I
  • Preparation method of magnetic molecularly imprinted polymer for separating/purifying sudan I

Examples

Experimental program
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Effect test

Embodiment 1

[0027] (1) Take 80mL H 2 O was added to the three-necked flask, deoxygenated by nitrogen for half an hour, and then 9.56g FeCl was added 3 ·6H 2 O and 5.60 g FeSO 4 ·7H 2 O, the three-necked bottle was placed in a 65°C constant temperature oil bath, stirred rapidly, and after 30 minutes, concentrated ammonia water was added dropwise to pH = 8-9, and the color gradually became black, and Fe was formed at this time 3 o 4 precipitation. The whole reaction process was carried out under the protection of nitrogen. After 1 h, use a strong magnet to settle the resulting granular product, separate the supernatant, and wash with H successively. 2 O and absolute ethanol to wash the precipitate to neutrality.

[0028] (2) Unmodified Fe 3 o 4 There are a large number of hydroxyl groups on the surface of the particles, which are not easy to disperse in organic solvents. When preparing molecularly imprinted polymers, they are mostly distributed on the surface of the polymer and are...

Embodiment 2

[0035] Weigh 1mmol of Sudan I and 4mmol of methacrylic acid (MAA), place in a 250mL Erlenmeyer flask, add 180mL of acetonitrile, and sonicate for half an hour to mix well. Then add 3.00mL of cross-linking agent ethylene glycol dimethacrylate (EGDMA), 10mL of acetonitrile-based magnetic fluid prepared in Example 1 and 1.0g of initiator azobisisobutyronitrile (AIBN), and continue to sonicate. Nitrogen gas was passed for 1 h to remove the oxygen in the system, and the reaction was carried out in an air shaker at 60° C. for 24 h. Take it out and cool it to room temperature, add methanol / acetic acid (85:15, V / V) solution to ultrasonic for 30min, filter through a Buchner funnel, then use the same solution for Soxhlet extraction for 48h until no Sudan I molecules can be detected, and then use acetone Repeated sedimentation to remove fine powder, and finally vacuum dried at 60°C for 24h.

Embodiment 3

[0037] Weigh 1mmol Sudan I and 4mmol methacrylic acid (MAA), place in a 250mL Erlenmeyer flask, add 180mL acetonitrile, and ultrasonically mix for half an hour. Then add 1.5mL of cross-linking agent ethylene glycol dimethacrylate (EGDMA), 10mL of acetonitrile-based magnetic fluid prepared in Example 1 and 1.0g of initiator azobisisobutyronitrile (AIBN), and continue to sonicate. Nitrogen was passed for 1 h to remove the oxygen in the system, and the reaction was carried out in an air shaker at 65° C. for 24 h. Take it out and cool it to room temperature, add methanol / acetic acid (85:15, V / V) solution and sonicate for 20min, filter through a Buchner funnel, and then perform Soxhlet extraction with the same solution for 48h until no Sudan I molecules can be detected, and then use acetone Repeated sedimentation to remove fine powder, and finally vacuum dried at 60°C for 24h.

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Abstract

The invention discloses a preparation method of a magnetic molecularly imprinted polymer for separating / purifying sudan I. The preparation method comprises the following steps of: dissolving the sudan I and methacrylic into acetonitrile; adding a cross-linking agent, an initiator and acetonitrile-based magnetic fluid; reacting in the absence of oxygen; separating microspheres generated by the reaction; and eluting the sudan I in the microspheres by adopting soxhlet extraction and drying. According to the preparation method disclosed by the invention, the magnetic fluid is added into the imprinted polymer; in the process of adsorbing and extracting a template molecule, the imprinted polymer can be directly added into a measured substance solution for adsorbing without being filled in a solid phase extraction column, and then imprinted polymer granules are gathered and separated by using a strong magnetic filed; and meanwhile, the imprinted polymer retains specific adsorption of the template molecule to the sudan, and thereby quick detection and analysis of the template molecule are realized.

Description

technical field [0001] The invention relates to a preparation method of a magnetic imprinted polymer, in particular to a preparation method of separating / purifying Sudan I magnetic molecular imprinted polymer. Background technique [0002] Sudan Red I is a kind of lipophilic azo industrial dye, which is often used in the color enhancement of solvents, oils, waxes, gasoline, shoes, floors, etc., because of its bright color, it is not easy to fade after use, and after being used for food dyeing, it can cause People have a strong appetite, so some unscrupulous food companies use Sudan Red I as a food additive. Common foods include chili powder, chili oil, red bean curd, red heart eggs, etc. Sudan Red is carcinogenic. The International Agency for Research on Cancer lists Sudan Red I as a Class III carcinogen. [0003] Molecular imprinting technology refers to the technology of preparing aggregates that are specific and selective for a specific target molecule. The surface of mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/06C08F222/14C08J9/26B01J20/285B01J20/30
Inventor 李蕾黄静张祖磊
Owner JIAXING UNIV
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