Method for detecting SERS of aflatoxin B1 molecules based on molecularly imprinted polymer coated gold core-shell nanoparticles

A gold nanoparticle and aflatoxin technology, applied in the field of surface-enhanced Raman detection, can solve the problems of low aggregation efficiency of aflatoxin B1 molecules, difficult to achieve stable detection results of aflatoxin B1, etc. specific effect

Active Publication Date: 2017-07-07
JIMEI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, relying solely on the aggregation of magnetic nanoparticles to aggregate free aflatoxin B1 molecules in...

Method used

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  • Method for detecting SERS of aflatoxin B1 molecules based on molecularly imprinted polymer coated gold core-shell nanoparticles
  • Method for detecting SERS of aflatoxin B1 molecules based on molecularly imprinted polymer coated gold core-shell nanoparticles
  • Method for detecting SERS of aflatoxin B1 molecules based on molecularly imprinted polymer coated gold core-shell nanoparticles

Examples

Experimental program
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Effect test

Embodiment 1

[0030] 1) Synthesis of gold nanoparticles with a particle size of 55±10nm:

[0031] Take 200mL of 0.01% chloroauric acid aqueous solution in a 250mL round bottom flask, heat to boiling under reflux under magnetic stirring, then quickly add 1.4mL of 1% sodium citrate aqueous solution. The solution in the round bottom flask changed from clear light gold to dark blue within half a minute, and continued to boil, the color of the solution gradually changed from dark blue to wine red until it became stable. Keep the boiling state for 40min, naturally cool to room temperature after making it completely react, and the gold nanoparticle sol (referring to figure 1 ).

[0032] 2) Synthesis of core-shell nanoparticles:

[0033] The gold sol synthesized in 1) was concentrated by centrifugation (centrifugation condition: 6000r / min, 5min). Take equal volume concentration as 3×10 -4 mol / L aflatoxin B1 solution with a concentration of 1.2×10 -3 After mixing the mol / L methacrylic acid solu...

Embodiment 2

[0038] 1) Synthesis of gold nanoparticles with a particle size of 55±10nm:

[0039] Take 200mL of 0.01% chloroauric acid aqueous solution in a 250mL round bottom flask, heat to boiling under reflux under magnetic stirring, then quickly add 1.4mL of 1% sodium citrate aqueous solution. The solution in the round bottom flask changed from clear light gold to dark blue within half a minute, and continued to boil, the color of the solution gradually changed from dark blue to wine red until it became stable. Keep the boiling state for 40 minutes, allow it to react completely, and then naturally cool to room temperature to obtain a gold nanoparticle sol with a particle size of 55±10 nm.

[0040] 2) Synthesis of core-shell nanoparticles:

[0041] The gold sol synthesized in 1) was concentrated by centrifugation (centrifugation condition: 6000r / min, 5min). Take equal volume concentration as 3×10 -4 The mol / L aflatoxin B1 solution is the same as the concentration of 1.2×10 -3 mol / L m...

Embodiment 3

[0045] 1) Synthesis of gold nanoparticles with a particle size of 55±10nm:

[0046] Take 200mL of 0.01% chloroauric acid aqueous solution in a 250mL round bottom flask, heat to boiling under reflux under magnetic stirring, then quickly add 1.4mL of 1% sodium citrate aqueous solution. The solution in the round bottom flask changed from clear light gold to dark blue within half a minute, and continued to boil, the color of the solution gradually changed from dark blue to wine red until it became stable. Keep the boiling state for 40 minutes, allow it to react completely, and then naturally cool to room temperature to obtain a gold nanoparticle sol with a particle size of 55±10 nm.

[0047] 2) Synthesis of core-shell nanoparticles (Au@MIP):

[0048] The gold sol synthesized in 1) was concentrated by centrifugation (centrifugation condition: 6000r / min, 5min). Take equal volume concentration as 3×10 -4 mol / L aflatoxin B1 solution with a concentration of 1.2×10 -3 After mixing t...

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Abstract

The invention provides a method for detecting SERS of aflatoxin B1 molecules based on molecularly imprinted polymer coated gold core-shell nanoparticles, and relates to surface reinforced raman detection. According to the method, core-shell nano-particles taking gold nanoparticles as a core and molecularly imprinted polymer as a shell are synthesized by coating aflatoxin B1 molecularly imprinted polymer around the gold nanoparticles; the hole in the molecularly imprinted polymer can be used for specifically capturing the aflatoxin B1 molecules after template molecules in the molecularly imprinted polymer of the shell layer are eluted, and surface reinforced raman detection of aflatoxin B1 molecules can be realized since the aflatoxin B1 molecules are in an effective range of a gold nanoparticle plasma resonant magnetic field; and the molecularly imprinted polymer is obtained by eluting the molecularly imprinted shell layer prepared by using the aflatoxin B1 molecules as template molecules, and has highly specific adsorption for aflatoxin B1, and can be used as a SERS substrate for quantitative detection of aflatoxin B1. The method has the characteristics of high specificity and short sample analyzing time.

Description

technical field [0001] The invention relates to the detection of surface-enhanced Raman, in particular to a SERS detection method for aflatoxin B1 molecules based on molecularly imprinted polymer gold-coated core-shell nanoparticles. Background technique [0002] Aflatoxin (AFT) is a class of mycotoxins, mainly secondary metabolites produced by Aspergillus flavus, Aspergillus parasiticus and Aspergillus nomius (Pan Zhonghua, Xu Yanfang, Cheng Hengsong. Progress in Analytical Methods of Aflatoxins [J]. Agricultural Environment and Development, 1995, (2): 30~33.). At present, 18 kinds of toxins such as AFB1, AFB2, AFB2a, AFG1, AFG2, AFG2a, AFM1, and AFM2 have been isolated and identified, among which aflatoxin B1 has the highest toxicity and carcinogenicity, and its toxicity is about cyanide 10 times that of potassium, 68 times that of arsenic, carcinogenicity is 75 times that of dimethylnitrosamine, and 900 times that of butter yellow (Zhang Wenling, Yuan Tao, Li Shuguo. Res...

Claims

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Application Information

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IPC IPC(8): C08F220/06C08F222/14C08F2/44C08K3/08C08J9/26G01N21/65B01J20/26B01J20/30
CPCB01J20/268C08F2/44C08F220/06C08J9/26C08J2201/0422C08J2333/02C08K3/08C08K2003/0831C08K2201/003C08K2201/011G01N21/658C08F222/102
Inventor 张芹孙文贺路影
Owner JIMEI UNIV
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