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Preparation method of surface-enhanced Raman scattering probe

A surface-enhanced Raman and probe technology, which is applied in the field of preparation of aggregated silver nanoprobes, can solve problems such as precipitation and unstable clusters, and achieve the effects of environmental friendliness, good repeatability, and low toxicity

Inactive Publication Date: 2010-04-14
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Aggregating metal nanoparticles can provide more "hot spots" to significantly increase the SERS intensity, however, excessive aggregation will lead to the formation of large unstable clusters of metal nanoparticles and rapid precipitation.

Method used

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  • Preparation method of surface-enhanced Raman scattering probe
  • Preparation method of surface-enhanced Raman scattering probe
  • Preparation method of surface-enhanced Raman scattering probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 probe preparation (taking rhodamine 6G probe as an example)

[0018] In the first step, the silver colloid solution was prepared by the method reported by Lee and Meisel. will be 1.0×10 -2 Add the silver nitrate solution to the silver colloid solution in a volume ratio of 1:10 into deionized water, stir and heat to boiling. Add 1% sodium citrate solution into the boiling silver nitrate solution at a volume ratio of 1:50 to the silver colloid solution, keep stirring and heat to boil for 40 minutes to obtain the silver colloid solution. The prepared silver colloidal solution was protected from light and sealed for future use.

[0019] The second step is to prepare 1 mM rhodamine 6G aqueous solution, take the rhodamine 6G solution and add it to the silver colloid solution at a volume ratio of 1:1000-1:100 to the silver colloid solution under magnetic stirring, and react for 5 minutes. Add 0.5M sodium chloride solution to the mixed solution of rhodamine 6G a...

Embodiment 2

[0020] The chemical stability detection of embodiment two probe (taking rhodamine 6G probe as example)

[0021] The first step is to do a group of comparative experiments on the stability of the absorption spectrum. The first group first prepares a 1mM rhodamine 6G aqueous solution, takes the rhodamine 6G solution and adds it to the silver colloid solution at a volume ratio of 1:500 to the silver colloid solution under magnetic stirring. React for 5 minutes. Then add a concentration of 0.5M sodium chloride solution according to the volume ratio of the silver colloid solution of 1:750; the second group adds 1% PVP aqueous solution with a molecular weight of 50,000 according to the volume ratio of the silver colloid solution of 1:150 on the basis of the first group. The absorption spectra of the solutions of the first group and the second group were recorded with time. It can be known from the change of the absorption spectrum intensity that if there is no protection of PVP, su...

Embodiment 3

[0023] Example 3 The SERS activity of the probe in living cells and the biocompatibility of the probe (the SERS activity in living cells is exemplified by the 4-mercaptobenzoic acid probe, and the cell survival rate is exemplified by the rhodamine 6G probe). The first step Cervical cancer cells (Hela) were cultured in culture medium (37°C, 5% CO 2 ). After 24 hours, add the 4-mercaptobenzoic acid SERS probe solution (the preparation method is the same as that of the rhodamine 6G probe solution) into the cell culture medium in a volume ratio (3:1), shake it gently, and put it back in the incubator Inside. The SERS probe enters the interior of the cell by being phagocytized by the cell. After 1.5 hours, the culture medium was aspirated, and the cells were washed 3 times with phosphate buffered saline (PBS) to remove the SERS probes that were not phagocytized by the cells and remained in the culture medium for use.

[0024]In the second step, the cells washed with the buffer s...

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Abstract

The invention provides a preparation method of a surface-enhanced Raman scattering probe, relating to a preparation method of a silver nano aggregation probe which has high surface-enhanced Raman scattering activity and is coated by polyvinylpyrrolidone, as well as applications thereof in regulation of aggregation degree of silver nano particles and SERS detection in cells. The method selects silver colloid solution as a raw material, selects sodium chloride as an aggregation agent, and coats the polyvinylpyrrolidone on the surface of silver aggregates; and specifically comprises the following steps: evenly mixing Raman markers and the silver colloid solution according to volume ratio of 1: 1000 to 1:100 under the stirring condition, then adding sodium chloride solution to aggregate the silver colloid solution mixed with the Raman markers into aggregates with two to five particles, finally adding the polyvinylpyrrolidone solution, continuously stirring for 5 to 15 minutes, filtering the obtained solution with a filter with the aperture specification of 100 to 220nm and then obtaining the surface-enhanced Raman scattering probe. The probe is water-soluble colloid solution.

Description

technical field [0001] The present invention relates to a preparation method of polyvinylpyrrolidone (Polyvinylpyrrolidone, PVP)-wrapped aggregated silver nanoprobe with high surface-enhanced Raman scattering (Surface enhanced Raman Scattering, SERS) activity, and its application to regulating the aggregation of silver nanoparticles Extent and detection of SERS in cells. More generally related to metallic nanomaterials, polymeric materials. Background technique [0002] In recent years, the detection of biological samples by designing optical probes with certain functions has been a research hotspot. Fluorescent probes are traditional optical probes. After a long period of development, fluorescent probes have been widely used. However, fluorescent probes have disadvantages such as narrow excitation spectrum, easy photobleaching of samples, and toxicity to biological samples when the concentration of fluorescent dye is too high. Compared with traditional fluorescence spect...

Claims

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Application Information

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IPC IPC(8): G01N21/65
Inventor 崔一平杨晶王著元谈学斌宋春元张若虎
Owner SOUTHEAST UNIV
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