A kind of SERS self-reference microfluidic chip based on magnetic graphene, its preparation method and application

A magnetic graphene and microfluidic chip technology, applied in chemical instruments and methods, laboratory containers, Raman scattering, etc., can solve problems that have not yet been found with similar technologies, achieve simplified calculations, reduce interference, and save consumables Effect

Inactive Publication Date: 2016-03-16
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • A kind of SERS self-reference microfluidic chip based on magnetic graphene, its preparation method and application
  • A kind of SERS self-reference microfluidic chip based on magnetic graphene, its preparation method and application
  • A kind of SERS self-reference microfluidic chip based on magnetic graphene, its preparation method and application

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Experimental program
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Embodiment 1

[0037] like figure 1 and figure 2 As shown, a SERS self-reference microfluidic chip based on magnetic graphene, including a substrate 1,

[0038] The substrate 1 is provided with a symmetrical reaction channel and a reference channel relative to the central axis of the substrate. The feed end of the reaction channel and the reference channel is communicated with the feeding pipe 101, and the first reactant sample loading pipe 111 and the second The reactant sample loading pipe 102 communicates with the feed pipe 101 respectively, and the SERS substrate material injection channel 110 communicates with the reaction microfluidic channel 108 through the microvalve 109;

[0039] The cover sheet 2 cooperates with the substrate 1, the magnetic device is placed on the cover sheet 2, and the magnetic graphene solution 106 injected into the reaction channel is drawn to flow by the magnetic force generated by the magnetic device.

[0040] The third stepper motor drives the injector 10...

Embodiment 2

[0050] A method for preparing a SERS self-referencing microfluidic chip based on magnetic graphene, the method comprising the following steps:

[0051] 1) Prepare the positive mold of the substrate: move the mold insert on the mold base according to the set size, leave a microcavity consistent with the size of the substrate, and fix a strip on the fixed mold base plate corresponding to the reaction channel and the reference channel. A core, thereby forming a male mold of the substrate;

[0052] 2) Prepare substrate 1: pour organosol into the substrate male mold prepared in step 1, and when the organosol solidifies, remove the mold to obtain substrate 1;

[0053] 3) Preparation of cover sheet 2: move the mold insert on the mold base according to the set size, leave a microcavity consistent with the size of the cover sheet, pour organic sol, cool and mold, demould, and obtain cover sheet 2;

[0054] 4) preparing magnetic graphene for subsequent use,

[0055] That is, a SERS se...

Embodiment 3

[0065] The steps for monitoring the hybridization process of DNA molecules using the SERS self-reference microfluidic chip based on magnetic graphene are as follows:

[0066] 1) Modify carboxyl-modified single-stranded DNAa (sequence ATTGGCCTACCG-COOH) into the reaction channel and the reference channel, as follows:

[0067] 1-1) Dissolving the DNAa chain in phosphate buffer solution (PBS) at pH=7.4 to prepare a DNAa solution with a molar concentration of 20Nm / L;

[0068] 1-2) Deposit amino-rich polyacrylamide hydrochloride (PAH, MW=15000) in the reaction channel by dissolving amino-rich polyacrylamide hydrochloride in deionized water to prepare 100 mg / ml solution was added dropwise into the reaction channel, and after the water evaporated naturally, polyallylamine hydrochloride was deposited in the reaction channel;

[0069] 1-3) Inject 200 μL of DNAa solution from the first reactant loading tube 111;

[0070] 1-4) Inject EDC and NHS successively from the second reactant lo...

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Abstract

The invention provides a magnetic graphene based SERS (Surface Enhanced Raman Scattering) self-reference micro-fluidic chip. The magnetic graphene based SERS self-reference micro-fluidic chip comprises a substrate; a reaction channel and a reference channel which are symmetric about a medium axis of the substrate are formed in the substrate; feeding ends of the reaction channel and the reference channel are communicated and then are communicated with a feeding pipe; a first reactant sample loading pipe and a second reactant sample loading pipe are respectively communicated with the feeding pipe; an SERS substrate material pouring channel is communicated with a reaction micro-fluidic channel through a micro valve; a cover plate is in match with the substrate; a magnetic device is positioned on the cover plate to generate magnetism to pull magnetic graphene poured into the reaction channel to flow. With the adoption of the magnetic graphene based SERS self-reference micro-fluidic chip, the SERS can be monitored in real time at different biochemical reaction stages, and thus the SERS self-reference function can be achieved.

Description

technical field [0001] The invention relates to the field of preparation of a microfluidic chip and nanomaterials, in particular to a SERS self-referencing microfluidic chip based on magnetic graphene, its preparation method and application. Background technique [0002] The technology of manipulating fluids in micron-scale channels is called microfluidics. Micro-channels, micro-pumps, micro-valves, micro-electrodes and other functional components are integrated on the chip plate through fine processing technology, and then a miniature full analysis system similar to an integrated circuit chip is constructed, which is called a microfluidic chip. Using microfluidic technology and microfluidic chips to carry out analysis and detection, compared with traditional detection methods, has many advantages such as fast and efficient, high density, high throughput, low consumption, and integration. This also makes microfluidic chip analysis technology known as an extremely important ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N21/65
Inventor 刘敏刘一曼潘礼庆朴红光杨先卫罗志会郑胜
Owner CHINA THREE GORGES UNIV
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