The invention discloses a flotation method for gold ore with a higher mining obturator content and aims to solve the problems of influence, caused by the fact that a dispenser is adopted in the traditional flotation process to eliminate an obturator medium, on the flotation environment, existence of adhesive foam and deterioration of the flotation environment. The flotation method comprises the steps of ore grinding, roughing, scavenging, primary concentration and second concentration, wherein during the ore grinding, an ore pulp pH regulator and a mining obturator inhibiter are added, a collecting agent and a foaming agent are added for adjusting the ore, the ore is stirred, the roughing and floatation operation is preformed, the ore pulp pH regulator is H2SO4, the mining obturator inhibiter is (NH4)2SO4, the foaming agent is terpenic oil, the collecting agent is Z-309 and Z-305. The surface characteristics of the ore are improved by reasonably combining and adding the agents, the mining oburator is better inhibited under the condition of lower ore pulp pH, the selective adsorption of the collecting agent on the targeted mineral, namely the gold ore, is enhanced, the gold content achieves 200-276.23g/ton, the gold recovery rate achieves 87.67-92.69%. The beneficiation method is simple in process and the on-site operation is easy.