Method for preparing glutamic acid surface molecularly imprinted polymer silica microspheres

A technology of surface molecular imprinting and silica gel microspheres, which is applied in the field of chemical analysis and testing, can solve the problem of separation and detection of L-glutamic acid without the preparation of L-glutamic acid molecular imprinting polymers, uneven coating of surface imprinting polymers, imprinting In order to achieve the effect of narrow molecular weight distribution, short adsorption/desorption time and simple operation

Active Publication Date: 2016-06-22
SOUTH CHINA NORMAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

The surface imprinted polymer prepared by this method has disadvantages such as uneven coating, wide molecular weight distribution of imprinted matter, and unstable performance.
Moreover, there is no preparation of L-glutamic acid molecularly imprinted polymers by surface molecular imprinting in the prior art to achieve the separation and detection of L-glutamic acid

Method used

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  • Method for preparing glutamic acid surface molecularly imprinted polymer silica microspheres
  • Method for preparing glutamic acid surface molecularly imprinted polymer silica microspheres
  • Method for preparing glutamic acid surface molecularly imprinted polymer silica microspheres

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preparation example Construction

[0032] A preparation method for molecularly imprinted polymer silica gel microspheres on the surface of glutamic acid, comprising the following steps:

[0033] (1) Using a dilute hydrochloric acid immersion method to remove impurities on the surface of the silica gel microspheres, increase the number of hydroxyl groups on the surface of the silica gel microspheres, and obtain activated silica gel microspheres;

[0034] (2) Surface silanization modification of activated silica gel microspheres with 4-chloromethylphenyltrimethoxysilane to obtain silanized silica gel microspheres;

[0035] (3) Using RAFT to perform RAFT functionalization treatment on the silanized silica gel microspheres to obtain RAFT functionalized silica gel microspheres grafted with dithioester bonds on the surface;

[0036] (4) Fully mix template molecules and functional monomers in a mixed solvent to self-assemble template molecules and functional monomers, then add cross-linking agent, initiator and RAFT f...

Embodiment

[0040] This embodiment takes L-glutamic acid molecule as an example and 2-acrylamide-2-methylpropanesulfonic acid as a functional monomer to describe the present invention in detail, but this does not limit the protection scope of the present invention.

[0041] see figure 1 , which is L-glutamic acid molecularly imprinted polymer silica gel microspheres (MIP / SiO 2 ) Schematic diagram of the preparation process. The preparation method comprises the following steps:

[0042] (1) Activation of silica gel microspheres

[0043] Accurately weigh 5g of silica gel microspheres and add them to a 250mL round-bottomed flask, then add 150mL of hydrochloric acid solution with a mass fraction of 8-15%, ultrasonicate for 10-20min, and then place the round-bottomed flask in oil at 105-125°C Put it in the bath and keep it for 20~28h. After the end, wash and filter with deionized water until neutral. Then, the silica gel microspheres are placed in a blast drying oven, and activated at 105...

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Abstract

The invention discloses a method for preparing glutamic acid surface molecularly imprinted polymer silica microspheres. The method includes the steps that a dilute hydrochloric acid soaking method is adopted, impurity removal is conducted on the surfaces of the silica microspheres, and activated silica microspheres are obtained; surface silanization modification is conducted on the activated silica microspheres, and silanization silica microspheres are obtained; RAFT functionalized processing is conducted on the silanization silica microspheres, and RAFT functionalized silica microspheres with the surfaces grafted with double sulphur ester bonds are obtained; template molecules and functional monomers are fully and uniformly mixed, then a cross-linking agent, initiator and the RAFT functionalized silica microspheres are added, a polymerization reaction occurs, and the molecularly imprinted polymer silica microspheres with the surfaces wrapped by the template molecules are obtained; elution is conducted to remove the template molecules. The surface molecularly imprinted polymer silica microspheres prepared through the method are uniform in wrapping and stable in performance, absorb L-glutamic acid selectively under a water environment and have the advantages of being short in absorption / desorption time and simple in operation.

Description

technical field [0001] The invention belongs to the field of chemical analysis and testing, and in particular relates to a preparation method of glutamic acid surface molecularly imprinted polymer silica gel microspheres. Background technique [0002] Glutamic acid is an essential amino acid that makes up protein. Among them, L-glutamic acid is an amino acid that is very important to the human body. In the human body, it participates in the metabolism of protein and sugar in the brain and promotes the oxidation process. At the same time, it can combine with ammonia in the blood to generate non-toxic glutamine, which has a good effect on the treatment of hepatic coma and severe liver insufficiency. At the same time, L-glutamic acid widely exists in some brewed foods, which is also of great significance for improving the flavor of foods. [0003] The traditional analysis methods of amino acids include ion-exchange chromatography, reversed-phase high-performance liquid chroma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F292/00C08F220/56C08F220/58
CPCC08F292/00C08F2438/03C08F2500/03C08F220/585
Inventor 胡小刚陈时远
Owner SOUTH CHINA NORMAL UNIVERSITY
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