Preparation method of graphene quantum dot fluorescent probe for paraquat detection

A technology of graphene quantum dots and fluorescent probes, applied in the field of materials science, can solve problems such as poor effect

Inactive Publication Date: 2021-10-29
HEFEI UNIV
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Problems solved by technology

The probe was used for the comparative detection of diquat, cypermethrin, chlorpyrifos and glyphosate isopropylamino, and the effect was poor, and the quenching constant was much smaller than that of paraquat for graphene quantum dots with red emission spectrum. Howeve

Method used

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  • Preparation method of graphene quantum dot fluorescent probe for paraquat detection
  • Preparation method of graphene quantum dot fluorescent probe for paraquat detection
  • Preparation method of graphene quantum dot fluorescent probe for paraquat detection

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specific Embodiment approach

[0058] The specific embodiment: a kind of preparation method of the graphene quantum dot fluorescent probe that is used for paraquat detection, it is characterized in that: described graphene quantum dot fluorescent probe has the maximum emission wavelength of red emission spectrum band and is 630nm, and The characteristic absorption peak of the visible spectrum of paraquat with herbicidal activity is at 630nm. The fluorescence emission spectrum of the graphene quantum dot fluorescent probe overlaps with the maximum absorption wavelength of paraquat. According to the principle of fluorescence resonance energy transfer, graphene quantum dots The energy donor in the fluorescent group of the fluorescent probe produces fluorescence emission, and transfers the energy non-radiatively to the nearby energy acceptor paraquat molecule in the ground state through the interaction between dipoles and dipoles, resulting in graphite The fluorescent intensity of the graphene quantum dot fluore...

Embodiment

[0063] Example: Using cherry blossom leaves as raw materials, graphene quantum dot fluorescent probes with red emission spectrum can be prepared after microwave digestion.

[0064]The first step is the preparation of graphene quantum dot fluorescent probes with red emission spectrum: cut the green plant leaves into 1.5cm, soak them in 50 mL of absolute ethanol, then stir continuously at 500 rpm for 30min, let stand for 15min after stirring, and then Centrifuge the resulting solution at 8000rpm for 10min, take 40mL of supernatant, and rotate the supernatant to evaporate to obtain a muddy solid. Measure 5mL of water and add it to the muddy solid, and heat it in a 1000W microwave oven 5min, and finally add ethanol to dissolve to obtain a fluorescent probe with graphene quantum dots in the red emission spectrum;

[0065] The second step is the preparation of paraquat solution: weigh 2.572g of paraquat, place it in a 100mL volumetric flask, and pipette the concentration of 0.1 mol∙...

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Abstract

A preparation method of a graphene quantum dot fluorescent probe for paraquat detection comprises the following steps: firstly, soaking oriental cherry leaves in absolute ethyl alcohol, stirring, centrifuging, taking supernatant, performing rotary evaporation to obtain chlorophyll, dispersing the chlorophyll in water, heating in a microwave oven, and finally dispersing the chlorophyll in ethyl alcohol to obtain the graphene quantum dot fluorescent probe with a red emission spectrum band. The maximum emission wavelength of the graphene quantum dot fluorescent probe is 630 nm, the maximum absorption peak position of a visible region of paraquat free radicals is also at the 630 nm position, the energy levels of a fluorescent probe donor and a paraquat free radical acceptor are overlapped, fluorescence resonance energy transfer occurs, and probe fluorescence quenching is caused. The probe is poor in effect when being used for comparing and detecting aquacide, cypermethrin, chlorpyrifos and glyphosate isopropylamino, however, the detection limit is 10<-9> mol.L<-1> when being used for high-selectivity recognition and high-sensitivity detection of paraquat free radicals. The preparation method is simple and easy to implement, high in selectivity, high in sensitivity and low in detection limit.

Description

technical field [0001] The invention relates to the field of material science, in particular to a preparation method of graphene quantum dots with a red emission spectrum band for paraquat detection. Background technique [0002] Paraquat (paraquat, referred to as PQ), also known as paraquat, has a good weeding effect and can be almost completely inactivated in the soil. However, after oral administration, paraquat is absorbed through the digestive tract and enters the body to make the human lungs Partial fibrosis is complete and is an irreversible process. The widespread use of paraquat pesticides has increased the yield of crops, while its residues have caused environmental pollution and endangered human health, and there is no specific antidote for the poisoning caused by paraquat. Therefore, it is urgent to find a fast and convenient method for the detection of paraquat Residue method. [0003] At present, the commonly used detection methods include surface-enhanced Ra...

Claims

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Application Information

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IPC IPC(8): C09K11/65B82Y20/00B82Y40/00G01N21/64
CPCC09K11/65B82Y20/00B82Y40/00G01N21/6428G01N2021/6432
Inventor 高大明汪志辉赵家东倪才雨张年玺杨俊宇程远王竞
Owner HEFEI UNIV
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