Method of producing gentamicin B with gentamicin B fermentation liquid

A kind of technology of gentamicin and fermented liquid is applied in the field of producing gentamicin B by using gentamicin B fermented liquid, which can solve the problem of affecting the quality of isopamicin finished products, the loss of gentamicin B, and the reduction of product yield. efficiency and other issues, to achieve the effect of reducing the cost of environmental protection treatment, high effective content, and low total production cost

Active Publication Date: 2018-02-16
HEILONGJIANG LIANSHUN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] 3 In the ion exchange process, ammonia solution is used for analysis, resulting in the loss of part of gentamicin B, which reduces the product yield
[0009] 4 There is no quality standard for finished gentamicin B produ

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1) Fermentation broth pretreatment

[0041] Gentamicin B fermentation broth 10m 3 , the fermentation unit is 803 mg / L, and the total fermentation amount is 8.03.

[0042] Use 10% phosphoric acid to adjust the pH of the gentamicin B fermentation broth to 3, and then add calcium oxide to adjust the pH of the fermentation broth to 5 after full stirring.

[0043] Add heavy metal precipitant SHH-003 in an amount of 1kg, continue to stir for 40 minutes, and then let stand for 80 minutes.

[0044] Add the filter aid perlite, the model is GK-110 coarse, the dosage is 30kg, stir for 30min, and then let stand for 20min.

[0045] Filtered through a plate frame to obtain gentamicin B solution 7.3m 3 .

[0046] Add 2.2m of gentamicin B solution 3 Chloroform, stirred for 60min, then stood still for 100min, filtered after layering to obtain gentamicin B solution 7.2m 3 . After testing, the fermentation unit is 1098mg / L.

[0047] The yield of fermentation broth pretreatment: 98...

Embodiment 2

[0065] 1) Fermentation broth pretreatment

[0066] Gentamicin B fermentation broth 10m 3 , the fermentation unit is 814mg / L, and the total fermentation amount is 8.14.

[0067] Use 15% phosphoric acid to adjust the pH of the gentamicin B fermentation broth to 3.5, and add calcium oxide to adjust the pH of the fermentation broth to 5.3.

[0068] Add heavy metal precipitant SHH-003, the dosage is 1.5kg, continue to stir for 45min, then let stand for 85min

[0069] Add the filter aid perlite, the model is GK-110 coarse, the dosage is 35kg, stir for 35min, and then let stand for 25min.

[0070] Filtered through a plate frame to obtain gentamicin B solution 7.4m 3 .

[0071] Add 2.6m of gentamicin B solution 3 Chloroform, stirred for 65min, then stood still for 105min, layered and filtered to obtain gentamicin B solution 7.3m 3 . After testing, the fermentation unit is 1098mg / L.

[0072] The yield of fermentation broth pretreatment: 98.5%.

[0073] 2) Ceramic membrane filt...

Embodiment 3

[0090] 1) Fermentation broth pretreatment

[0091] Gentamicin B fermentation broth 10m 3 , the fermentation unit is 821mg / L, and the total fermentation amount is 8.21.

[0092] Use 20% phosphoric acid to adjust the pH of the gentamicin B fermentation broth to 4, and add calcium oxide to adjust the pH of the fermentation broth to 5.5.

[0093] Add heavy metal precipitant SHH-003, the dosage is 2kg, continue to stir for 50min, and then let stand for 90min

[0094] Add the filter aid perlite, the model is GK-110 coarse, the dosage is 40kg, stir for 40min, and then let stand for 30min.

[0095] Filtered through a plate frame to obtain gentamicin B solution 7.3m 3 .

[0096] Add 2.9m of gentamicin B solution 3 Chloroform, stirred for 70min, then stood still for 110min, layered and filtered to obtain gentamicin B solution 7.2m 3 . After testing, the fermentation unit is 1124mg / L.

[0097] The yield of fermentation broth pretreatment: 98.6%.

[0098] 2) Ceramic membrane filt...

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PUM

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Abstract

The invention relates to a method of producing gentamicin B with gentamicin B fermentation liquid. The method includes the steps of: 1) regulating pH value of the gentamicin B fermentation liquid to 3-5 with phosphoric acid, fully stirring the fermentation liquid, adding CaO to regulate the pH of the fermentation liquid to 5-6, fully stirring the fermentation liquid, successively adding a heavy metal precipitant and a co-filtering agent to treat the fermentation liquid, and performing plate-and-frame filtration and chloroform layering to obtain a gentamicin B water solution; 2) performing ceramic membrane filtration, expanded bed adsorption, crystallization and drying to produce a gentamicin B pure product. The method achieves stable and high-effective production of the gentamicin B, increases extraction yield and effective content of the gentamicin B, is simple in processes and low in production cost, reduces environmental pollution index and reduces environmental protection treatmentcost.

Description

technical field [0001] The invention belongs to the technical field of antibiotic extraction, in particular to a method for producing gentamicin B by using gentamicin B fermentation liquid. Background technique [0002] Gentamicin B is an aminoglycoside antibiotic produced by the fermentation of a mutant of Micromonas aculeatus, and it is a small component of the gentamicin family. Gentamicin B is used to synthesize isopamicin, and isopamicin is on the rise due to its stability to most aminoglycoside inactivating enzymes produced by bacteria. [0003] Gentamicin B is a kind of alkaline, highly water-soluble aminoglycoside antibiotics. In the prior art, ion exchange method is mostly used to extract, separate and purify it: weakly acidic ion-exchange resins are used abroad, and domestic large Most factories use strong acid 732 resin for static adsorption and dynamic desorption. With the continuous research on the purification method of gentamicin, many new methods and techno...

Claims

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Application Information

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IPC IPC(8): C07H15/236C07H1/06
CPCC07H1/06C07H15/236
Inventor 任勇
Owner HEILONGJIANG LIANSHUN BIOTECHNOLOGY CO LTD
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