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Polyamine-modified chitosan base expanded bed adsorbing medium and preparation method thereof

An expanded bed adsorption, chitosan technology, applied in chemical instruments and methods, other chemical processes, alkali metal oxides/hydroxides, etc., can solve the problems of high price and narrow flow rate operating range, and achieve improved adsorption efficiency, promote large-scale separation and purification, improve adsorption separation efficiency and selectivity

Inactive Publication Date: 2013-10-30
SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the matrix materials of expanded bed adsorbents are mostly agarose, cellulose, silica gel, etc., and there are no reports on the application of chitosan.
The commercial expanded bed adsorption medium is mostly the Streamline series medium provided by GE Healthcare, which uses quartz sand as the weight-increasing inner core, and the outer layer is cross-linked agarose. The appearance is spherical and the particle size is between 100-300 μm, but the price is expensive.
In addition, the Streamline series resins still have the disadvantage of a narrow flow rate operating range

Method used

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  • Polyamine-modified chitosan base expanded bed adsorbing medium and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0020] Mix 100ml of chitosan solution with a mass fraction of 5% with 20g of stainless steel powder and 3g of PEG2000, then add 100ml of liquid paraffin, 15 drops of span-80, stir at high speed for 30min to make the system into an emulsion, then add 11ml of formaldehyde, and stir for 1h Pour the emulsion into a mixture of 10% NaOH / dehydrated ethanol (V(NaOH):V(dehydrated ethanol)=1:1) and let it stand for 1h, then wash it thoroughly with xylene, dehydrated ethanol and deionized water. Sieve to obtain chitosan / stainless steel powder composite microspheres with a particle size of 50-300 μm as a matrix; mix the drained composite microspheres, 50ml methanol, and 5ml benzaldehyde, react in a shaker at 200rpm at 60°C for 12h, and then add boron React sodium cyanide at room temperature for 12 hours, suction filter, and wash to obtain benzaldehyde substrate; then mix benzaldehyde substrate, 100ml 0.4mol / L NaOH solution, and 40ml epichlorohydrin, react at 50°C for 4 hours, suction filte...

Embodiment 2

[0022] Mix 100ml of chitosan solution with a mass fraction of 5% with 35g of stainless steel powder and 3g of PEG2000, then add 100ml of liquid paraffin, 15 drops of span-80, stir at high speed for 30min to make the system into an emulsion, then add 11ml of formaldehyde, and stir for 1h Pour the emulsion into a mixture of 10% NaOH / dehydrated ethanol (V(NaOH):V(dehydrated ethanol)=1:1) and let it stand for 2 hours, then wash it thoroughly with xylene, dehydrated ethanol and deionized water. Sieve to obtain chitosan / stainless steel powder composite microspheres with a particle size of 50-300 μm as the matrix; mix the drained composite microspheres, 50ml methanol, and 5ml benzaldehyde, react in a shaker at 200rpm at 60°C for 8h, and then add boron React sodium cyanide at room temperature for 12 hours, suction filter, and wash to obtain benzaldehyde substrate; then mix benzaldehyde substrate, 100ml 0.4mol / L NaOH solution, and 25ml epichlorohydrin, react at 50°C for 4 hours, suction...

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Abstract

The invention provides a polyamine-modified chitosan base expanded bed adsorbing medium and a preparation method of the polyamine-modified chitosan base expanded bed adsorbing medium, and relates to an adsorbing medium and a preparation method of the adsorbing medium. The matrix is a chitosan / stainless steel powder composite micro-ball, and the genin is induced phenyl by activating benzaldehyde and the re-coupled polyethylene polyamine by activating epichlorohydrin. The preparation method comprises the following steps of: adopting a reversed phase suspension crosslinking method to prepare the chitosan / stainless steel powder composite micro-ball which is taken as a blank matrix; and adopting a method that two genins are designed in a dividing way to firstly react chitosan unit molecule C2 site amino in the micro-ball matrix with benzaldehyde, build the hydrophobic interacted genin, activate matrix chitosan unit molecule C6 site hydroxyl by epichlorohydrin, and couple polyethylene polyamine, so as to obtain the expanded bed adsorbing medium which takes the phenyl and the polyethylene polyamine as the genins. The expanded bed adsorbing medium provided by the invention has the ion exchange genin and the hydrophobic genin, and the absorbing and separating efficiency and the selectivity of target matter in feed liquid can be improved, so that the biological substance such as the protein and the like can be separated and purified on a large scale.

Description

technical field [0001] The present invention relates to an adsorption medium and a preparation method thereof, in particular to an expanded bed adsorption medium and a preparation method which uses phenyl and polyamine as ligands for the separation of biological substances, and belongs to the expanded bed adsorption separation technology in chromatography separation . Background technique [0002] Bioseparation technology is an important part of bioengineering technology. Improving bioseparation technology, purifying target products with low cost, high yield, and high efficiency, and effectively controlling the content of harmful impurities are the key to whether biologically active substances can be transformed into products with practical value. and competitive products are the primary issues. At the same time, for the expanded bed adsorption medium, it is also very important to add new media, expand greater uses, and improve reaction quality and efficiency. [0003] Exp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/24B01J20/28B01J20/30
Inventor 朱建星王红艳
Owner SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
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