A hybrid gel of polylactic acid microspheres and cross-linked hyaluronic acid for injection and its preparation method

A technology for cross-linking hyaluronic acid and polylactic acid microspheres, applied in the field of medicine, can solve the problems of not being able to achieve long-term beauty, the preparation process is cumbersome, and the treatment effect is not obvious, and can reduce the amount of solvent cross-linked hyaluronic acid or its Effects of the use of salt, strengthening of the internal structure of the skin, ease of control of the conditions of the preparation process

Inactive Publication Date: 2015-12-30
河北善远科技有限公司
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Problems solved by technology

[0003] Patent 200810009190.8 discloses a suspension of hyaluronic acid or its salt containing macromolecular hydrogel for injection and its preparation method, but its macromolecule is a single-component cross-linked polysaccharide compound or polymer, and the therapeutic effect is not good. Obvious, and hyaluronic acid is easy to diffuse in the body and be degraded by enzymes in body tissues, the retention time is short, and it cannot achieve long-term beauty effects
Patent 200810009193.1 discloses a mixed gel for injection and its preparation method. The preparation process is cumbersome, and hyaluronic acid or its salt is used as a solvent, which has a short retention time in the body and cannot achieve long-term beauty effects.

Method used

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  • A hybrid gel of polylactic acid microspheres and cross-linked hyaluronic acid for injection and its preparation method

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[0017] The invention relates to a mixed gel of polylactic acid microspheres and cross-linked hyaluronic acid for injection and a preparation method thereof.

[0018] A hybrid gel of polylactic acid microspheres and cross-linked hyaluronic acid for injection, consisting of a salt solution of polylactic acid microspheres and cross-linked hyaluronic acid gel; the molecular weight of the polylactic acid microspheres is 15000-120000, and the average diameter is 10-150 μm, and the mass fraction in the mixed gel is 5-25%; the salt solution of the cross-linked hyaluronic acid gel is a cross-linked hyaluronic acid gel at an osmotic pressure of 250-350 mOsmol / L, It is obtained by swelling and balancing in sodium chloride solution or phosphate buffer solution with a pH of 6.5-7.5; the cross-linked hyaluronic acid gel is divinyl sulfone or glycidyl ether cross-linked hyaluronic acid gel.

[0019] The preferred mass fraction of polylactic acid microspheres in the mixed gel is 6-15%.

[00...

Embodiment 1

[0029] Add 5g of polylactic acid with a molecular weight of 15,000 to 150ml of dichloromethane and dissolve it. Dissolve another 2.5g of polyvinyl alcohol in 500ml of water and dissolve it in a water bath at 75°C. Add the polylactic acid solution dropwise to the aqueous solution of polyvinyl alcohol at a rate of 40 Drops per minute, after the dropwise addition was completed, the reaction was stirred for 2 hours, and the reaction temperature was 75°C. After the reaction was completed, dichloromethane was removed to obtain the reaction solution, and the reaction solution was filtered to collect the filter cake to obtain 4.8g of polylactic acid microspheres;

[0030] Dissolve 4g of hyaluronic acid (average molecular weight 1.6 million) in 160ml of 0.5N dilute sodium hydroxide solution, then add 1g of divinyl sulfone, and stir to obtain cross-linked hyaluronic acid;

[0031] Soak the cross-linked hyaluronic acid with a phosphate buffer solution with an osmotic pressure of 250mOsmol...

Embodiment 2

[0034] Add 5g of polylactic acid with a molecular weight of 120,000 to 175ml of dichloromethane and dissolve it. Dissolve another 7.5g of polyvinyl alcohol in 500ml of water and dissolve it in a water bath at 85°C. Add the polylactic acid solution dropwise to the aqueous solution of polyvinyl alcohol at a rate of 30 Drops per minute, after the dropwise addition was completed, the reaction was stirred for 4 hours, the reaction temperature was 85°C, and the methylene chloride was removed at the end of the reaction to obtain the reaction liquid, and the reaction liquid was filtered to collect the filter cake to obtain 4.9g of polylactic acid microspheres;

[0035] Dissolve 4g of hyaluronic acid (average molecular weight 2 million) in 140ml of 1.5N dilute sodium hydroxide solution, then add 2g of divinyl sulfone and stir to obtain cross-linked hyaluronic acid;

[0036] Soak the cross-linked hyaluronic acid with a phosphate buffer solution with an osmotic pressure of 350mOsmol / L and...

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Abstract

The invention relates to a mixed gel of polylactic acid microspheres and cross-linked hyaluronic acid for injection and a preparation method of the mixed gel. The polylactic acid microspheres have the molecular weight of 15000-120000 and the average particle size of 10-150mu m, and the mass fraction of the polylactic acid microspheres in the mixed gel is 5-25%; a salt solution of the cross-linked hyaluronic acid gel is obtained by swelling and balancing the cross-linked hyaluronic acid gel in a sodium chloride solution or a phosphate buffer solution with the osmotic pressure of 250-350mOsmol / L and the pH value of 6.5-7.5; the cross-linked hyaluronic acid gel is a hyaluronic acid gel cross-linked by divinyl sulfone or glycidyl ether. A mixed gel product of polylactic acid and cross-linked hyaluronic acid, obtained by directly mixing the polylactic acid microspheres with the cross-linked hyaluronic acid gel obtained by swelling and balancing in the salt solution is uniform, fine, long in partial retention time, good in plasticity and few in side effects, has an obvious effect on eliminating wrinkles, and has few operation steps and stable product quality.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a mixed gel of polylactic acid microspheres and cross-linked hyaluronic acid for injection and a preparation method thereof. Background technique [0002] In recent years, injectable gels for cosmetic or medical purposes have developed rapidly. Injectable gels can achieve minimally invasive treatment or filling cosmetic effects at the injection site, and have attracted widespread attention. However, in order to achieve the above goals, higher requirements are put forward for the materials used as injectable gels. Compared with animal-derived materials, artificial chemically synthesized polymer materials are safe to use because they reduce the risk of infection and disease. Its scope of use is gradually expanding, but synthetic polymer materials should have good biocompatibility and biodegradability before they can be used in hybrid gels. [0003] Patent 200810009190.8 discloses a suspensio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/04A61L31/06A61L31/14
Inventor 李红梅郈秀菊王晓晨
Owner 河北善远科技有限公司
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