Mixed gel of polylactic acid microspheres and cross-linked hyaluronic acid for injection and preparation method of mixed gel

A technology of cross-linked hyaluronic acid and polylactic acid microspheres, which is applied in the field of medicine, can solve the problems of inability to achieve long-term beauty, cumbersome preparation process, and inconspicuous therapeutic effect, and achieve the reduction of solvent cross-linked hyaluronic acid or its The use of salt, the strengthening of the internal structure of the skin, the effect of easy control of the preparation process conditions

Inactive Publication Date: 2015-01-07
河北善远科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Patent 200810009190.8 discloses a suspension of hyaluronic acid or its salt containing macromolecular hydrogel for injection and its preparation method, but its macromolecule is a single-component cross-linked polysaccharide compound or polymer, and the therapeutic effect is not good. Obvious, and hyaluronic acid is easy to diffuse in the body and be degraded

Method used

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preparation example Construction

[0032] The present invention also relates to a preparation method of polylactic acid microspheres for injection and cross-linked hyaluronic acid mixed gel, comprising the following steps:

[0033] (1) Preparation of polylactic acid microspheres;

[0034] (2) Preparation of cross-linked hyaluronic acid;

[0035] (3) Fully swell and balance the cross-linked hyaluronic acid obtained in step (2) in a salt solution to obtain a salt solution of a cross-linked hyaluronic acid gel;

[0036] (4) Mix the polylactic acid microspheres obtained in step (1) with the cross-linked hyaluronic acid salt solution obtained in step (3), and sub-package after sterilization to obtain a mixed gel of polylactic acid microspheres and cross-linked hyaluronic acid for injection .

[0037] A preferred preparation method of polylactic acid microspheres for injection and cross-linked hyaluronic acid hybrid gel comprises the following steps:

[0038] (1) Add polylactic acid with a molecular weight of 15,0...

Embodiment 1

[0045] Add 5g of polylactic acid with a molecular weight of 15,000 to 150ml of dichloromethane and dissolve it. Dissolve another 2.5g of polyvinyl alcohol in 500ml of water and dissolve it in a water bath at 75°C. Add the polylactic acid solution dropwise to the aqueous solution of polyvinyl alcohol at a rate of 40 Drops per minute, after the dropwise addition was completed, the reaction was stirred for 2 hours, and the reaction temperature was 75°C. After the reaction was completed, dichloromethane was removed to obtain the reaction solution, and the reaction solution was filtered to collect the filter cake to obtain 4.8g of polylactic acid microspheres;

[0046] Dissolve 4g of hyaluronic acid (average molecular weight 1.6 million) in 160ml of 0.5N dilute sodium hydroxide solution, then add 1g of divinyl sulfone, and stir to obtain cross-linked hyaluronic acid;

[0047] Soak the cross-linked hyaluronic acid with a phosphate buffer solution with an osmotic pressure of 250mOsmol...

Embodiment 2

[0050] Add 5g of polylactic acid with a molecular weight of 120,000 to 175ml of dichloromethane and dissolve it. Dissolve another 7.5g of polyvinyl alcohol in 500ml of water and dissolve it in a water bath at 85°C. Add the polylactic acid solution dropwise to the aqueous solution of polyvinyl alcohol at a rate of 30 Drops per minute, after the dropwise addition was completed, the reaction was stirred for 4 hours, the reaction temperature was 85°C, and the methylene chloride was removed at the end of the reaction to obtain the reaction liquid, and the reaction liquid was filtered to collect the filter cake to obtain 4.9g of polylactic acid microspheres;

[0051] Dissolve 4g of hyaluronic acid (average molecular weight 2 million) in 140ml of 1.5N dilute sodium hydroxide solution, then add 2g of divinyl sulfone and stir to obtain cross-linked hyaluronic acid;

[0052] Soak the cross-linked hyaluronic acid with a phosphate buffer solution with an osmotic pressure of 350mOsmol / L and...

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Abstract

The invention relates to a mixed gel of polylactic acid microspheres and cross-linked hyaluronic acid for injection and a preparation method of the mixed gel. The polylactic acid microspheres have the molecular weight of 15000-120000 and the average particle size of 10-150mu m, and the mass fraction of the polylactic acid microspheres in the mixed gel is 5-25%; a salt solution of the cross-linked hyaluronic acid gel is obtained by swelling and balancing the cross-linked hyaluronic acid gel in a sodium chloride solution or a phosphate buffer solution with the osmotic pressure of 250-350mOsmol/L and the pH value of 6.5-7.5; the cross-linked hyaluronic acid gel is a hyaluronic acid gel cross-linked by divinyl sulfone or glycidyl ether. A mixed gel product of polylactic acid and cross-linked hyaluronic acid, obtained by directly mixing the polylactic acid microspheres with the cross-linked hyaluronic acid gel obtained by swelling and balancing in the salt solution is uniform, fine, long in partial retention time, good in plasticity and few in side effects, has an obvious effect on eliminating wrinkles, and has few operation steps and stable product quality.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a mixed gel of polylactic acid microspheres and cross-linked hyaluronic acid for injection and a preparation method thereof. Background technique [0002] In recent years, injectable gels for cosmetic or medical purposes have developed rapidly. Injectable gels can achieve minimally invasive treatment or filling cosmetic effects at the injection site, and have attracted widespread attention. However, in order to achieve the above goals, higher requirements are put forward for the materials used as injectable gels. Compared with animal-derived materials, artificial chemically synthesized polymer materials are safe to use because they reduce the risk of infection and disease. Its scope of use is gradually expanding, but synthetic polymer materials should have good biocompatibility and biodegradability before they can be used in hybrid gels. [0003] Patent 200810009190.8 discloses a suspensi...

Claims

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Application Information

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IPC IPC(8): A61L31/04A61L31/06A61L31/14
Inventor 李红梅郈秀菊王晓晨
Owner 河北善远科技有限公司
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