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Preparation method of composite crosslinking sodium hyaluronate gel microspheres for facial injection

A technology of sodium hyaluronate and gel microspheres, which is applied in the field of medical materials, can solve the problem of unsatisfactory biocompatibility and water retention performance, does not meet market demands, and cross-linked hyaluronic acid microspheres do not carry out effective oil phase residues. and cross-linking agent residue control, to achieve good biocompatibility, water retention, good elasticity, and large uniformity.

Inactive Publication Date: 2015-03-04
杨子中
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The shape of microbeads obtained by this method is uniform, and the particle size distribution is between 1nm and 1μm. And the water retention performance is still unsatisfactory, still does not meet the market demand

Method used

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Examples

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Effect test

preparation example Construction

[0023] A preparation method of composite cross-linked sodium hyaluronate gel microspheres for facial injection of the present invention comprises the following steps:

[0024] (1) Sodium hyaluronate is prepared by microbial fermentation, sodium hyaluronate is prepared into sodium hyaluronate lye gel with a concentration of 10%-30% g / ml, and then the lye gel is added to the lye gel containing In the oil phase of the emulsifier, the oil phase can be one of vegetable oil, mineral oil, silicone oil or synthetic oil, preferably liquid paraffin or dodecane; the emulsifier is selected from the group with a hydrophilic and hydrophobic balance of 4 -7 emulsifier, preferably an emulsifier with a hydrophilic-hydrophobic balance of 5-6. The mass percentage of the emulsifier in the oil phase is 1-6%, preferably 3-5%; the weight ratio of the sodium hyaluronate lye gel to the oil phase is 1:1-1:10, preferably 1:1-1:5. Then it is emulsified at a high speed by a shearing machine, the shearin...

Embodiment 1

[0032] The preparation method of composite cross-linked sodium hyaluronate gel microspheres for facial injection comprises the following steps:

[0033] (1) Sodium hyaluronate is prepared by microbial fermentation, and sodium hyaluronate is prepared into sodium hyaluronate lye gel with a concentration of 10% g / ml, and then the lye gel is added to the emulsifier-containing In the oil phase, the oil phase is liquid paraffin; the emulsifier is selected from emulsifiers with a hydrophilic and hydrophobic balance value of 4, and the mass percentage of the emulsifier in the oil phase is 2%, and the sodium hyaluronate The weight ratio of the lye gel to the oil phase is 1:1. Then it is emulsified at a high speed by a shearing machine, the shearing machine speed is 50rpm, and the time is 10 minutes to form an emulsion of sodium hyaluronate microsphere particles; finally, the emulsion is fully stirred, and the pH value is adjusted to 8 with NaOH solution, and it is allowed to stand Bub...

Embodiment 2

[0041] The preparation method of composite cross-linked sodium hyaluronate gel microspheres for facial injection comprises the following steps:

[0042] (1) Sodium hyaluronate is prepared by microbial fermentation, sodium hyaluronate is formulated into sodium hyaluronate lye gel with a concentration of 30% g / ml, and then the lye gel is added to the emulsifier-containing In the oil phase, the oil phase is dodecane; the emulsifier is selected from a group with a hydrophilic-hydrophobic balance value of 7. The mass percentage of the emulsifier in the oil phase is 3%; the weight ratio of the sodium hyaluronate lye gel to the oil phase is 1:3. Then it is emulsified at a high speed by a shearing machine, the shearing machine speed is 200rpm, and the time is 20 minutes to form an emulsion of sodium hyaluronate microsphere particles; finally, the emulsion is fully stirred, and the pH value is adjusted to 11 with KOH solution, and it is allowed to stand Bubbles are removed, and the fi...

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Abstract

The invention discloses a preparation method of composite crosslinking sodium hyaluronate gel microspheres for facial injection. The preparation method comprises the following steps: (1) preparing sodium hyaluronate into 10-30 percent g / ml sodium hyaluronate alkali liquor gel, adding an oil phase containing an emulsifier, carrying out emulsification at a high speed by a shearing machine to form an emulsion liquid of sodium hyaluronate microsphere particles, fully mixing, regulating a pH value to be 8-11 by using an alkaline solution, and standing to remove bubbles; (2) dissolving polyvinyl alcohol in hot water for injection with concentration to be 10-20 percent (m / v); (3) uniformly mixing the emulsion liquid of the sodium hyaluronate microsphere particles obtained in the first step and divinyl sulphone as a crosslinking agent according to the ratio of 2:1, standing to remove bubbles, and centrifugally removing the oil phase; (4) adding a polyvinyl alcohol solution obtained in the second step in the microsphere particle gel obtained in the third step according to the ratio of (1:4)-(4:1), mixing, and removing bubbles; and (5) washing the microsphere particles obtained in the fourth step by a soluble organic solvent, removing the residual oil phase, and regulating a pH value by the alkaline solution to be neutral.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a method for preparing composite crosslinked sodium hyaluronate gel microspheres for facial injection. Background technique [0002] Hyaluronic acid is an acidic mucopolysaccharide. In 1934, Meyer, a professor of ophthalmology at Columbia University in the United States, first isolated the substance from the bovine vitreous. With its unique molecular structure and physical and chemical properties, hyaluronic acid shows a variety of important physiological functions in the body, such as lubricating joints, regulating the permeability of blood vessel walls, regulating protein, water and electrolyte diffusion and operation, and promoting wound healing. More importantly, hyaluronic acid has a special water-retaining effect and is the most moisturizing substance found in nature. It is known as an ideal natural moisturizing factor (Natural moisturizing factor, NMF), for examp...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08J3/12C08J3/075C08L5/08C08L29/04C08K5/41A61L27/26A61L27/52
Inventor 杨子中
Owner 杨子中
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