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Microscopic authentication method for stem strength of paeonia lactiflora pall cut flower

An identification method and cutting flower technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of differences, reliability effects, low consistency, etc., and achieve the effect of low error, high feasibility, and no interference from human factors.

Active Publication Date: 2018-12-21
YANGZHOU UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are mainly two widely used methods for judging the strength of plant stems: one is based on the perception of the human body, and the strength is judged by the feeling of bending the stem by hand. There are differences, so the results of stem strength determination are quite different, and scientific and effective data cannot be obtained; the other is based on mechanics, using a stem strength tester for measurement, which mainly uses pressure sensors to detect the bending of stems This method is simple and convenient to implement, but the consistency of manual operation is very low, the reliability of the results is greatly affected, and the instrument has certain limitations on the measured stems, some young stems Stalk cannot show readings due to too low intensity

Method used

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  • Microscopic authentication method for stem strength of paeonia lactiflora pall cut flower
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  • Microscopic authentication method for stem strength of paeonia lactiflora pall cut flower

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Embodiment 1

[0025] (1) Instruments and reagents:

[0026] YW-042-L Electric Blast Drying Oven (Shanghai Yuanwo Instrument Equipment Co., Ltd.), XL-30ESEM Scanning Electron Microscope (Netherlands Philips Company), LKB-5 Ultrathin Microtome (Sweden LKB Company), CM100 Transmission Electron Microscope (Philips Company of the Netherlands), YR / FL-680 refrigerator (Beijing Yiran Refrigeration Equipment Co., Ltd.), CPD-300 critical point drying instrument (Leica Company of Germany), and SCD 500 ion sputtering instrument (Leica Company of Germany).

[0027] Pentylene Glycol (AR Grade Analytical Pure (Shanghai Test), Shanghai Sinopharm Group), Potassium Dihydrogen Phosphate (AR Grade Analytical Pure (Shanghai Test), Shanghai Sinopharm Group), Anhydrous Sodium Sulfate (AR Grade Analytical Pure, Shanghai Shucan Industrial Co., Ltd.), Epon-812 epoxy resin (HEXION company in the United States), paraformaldehyde, acetone, absolute ethanol, tert-butanol, lead osmate nitrate, and trisodium citrate were ...

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Abstract

The invention discloses a microscopic authentication method for the stem strength of a paeonia lactiflora pall cut flower. The method comprises the following steps that: (1) fixing the stem of the paeonia lactiflora pall cut flower: cutting off the stem of the paeonia lactiflora pall cut flower, and fixing in pentanediol and paraformaldehyde solution; (2) observing the number of cell layers of which the xylem secondary wall is thickened; (3) observing the thickness of the secondary cell wall of which the xylem is thickened; and (4) carrying out statistical analysis on the number of cell layersof which the xylem secondary wall is thickened of the paeonia lactiflora pall cut flower and the thickness of the secondary cell wall, and through the two indexes, authenticating the stem strength ofthe paeonia lactiflora pall cut flower. The method can directly carry out observation and authentication according to a microscopic structure so as to be simple, convenient, free from human factor interference and low in error; and in addition, the method is free from the restriction of time, samples can be preserved for a long time and observed, in addition, repeated measurement can be carried out, and the method is high in feasibility.

Description

technical field [0001] The invention relates to a microcosmic identification method for the stem strength of peony cut flowers, and belongs to the technical field of peony stem strength detection methods. Background technique [0002] Paeonia lactiflora (Paeonia lactiflora Pall.) is one of the top ten traditional famous flowers in my country. In ancient times, there is a famous saying "Paeonia lactiflora is the favorite of spring, never envy peony" to praise the beauty of peony. In recent years, peony has become a high-end product in the flower market, and the supply exceeds demand in the international cut flower market (Sun Jing, 2018). Stem erection is an important quality indicator in most cut flower production. For example, in the production of cut gerbera flowers, large and heavy inflorescences and thin flower stems often lead to the phenomenon of "bent neck" in flower stems. Similar problems also occur in the production of peony cut flowers. The flower stems of some ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/22G01N23/2202
CPCG01N23/22G01N23/2202
Inventor 赵大球夏星陶俊
Owner YANGZHOU UNIV
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