The invention relates to application of a populus trichocarpa PtrbHLH186
gene in regulation and control of secondary
xylem development of trees, and belongs to the technical field of
gene engineering. In order to solve the problems that the need for wood quantity and quality is increased day by day and the biological function of the PtrbHLH186
transcription factor is unknown, a PtrbHLH186 overexpressed populus trichocarpa transgenic
plant is obtained through an
agrobacterium-mediated populus trichocarpa genetic transformation
system, and then a series of technical means are utilized for analysis and discovery that the PtrbHLH186 overexpressed populus trichocarpa transgenic
plant is found to have the advantages that the PtrbHLH186 overexpressed populus trichocarpa transgenic
plant; the overexpression of the PtrbHLH186
transcription factor affects the growth and development of plants and the formation of wood, which is mainly embodied in that the
lignin synthase
gene is regulated by the gene to affect the deposition of
lignin in the
secondary cell wall of a tree, so that the morphological structure and distribution of
xylem cells are changed; the results show that the PtrbHLH186
transcription factor has a certain regulation effect on the development of the secondary
xylem of the tree. The research content provided by the invention has important theoretical guiding significance for cultivating new varieties of high-quality woods and forests.