Method for preparing nano hydroxyapatite/collagen scaffold with directionally arranged particles

A nano-hydroxyapatite, directional alignment technology, applied in medical science, prosthesis, etc., can solve the problem of inability to effectively realize the bionic construction of nano-hydroxyapatite and collagen, the inability to form effective bonds with the collagen matrix, and the problem of hydroxyapatite Problems such as easy agglomeration of stone powder, to achieve good biocompatibility and physiological safety, excellent dispersion stability, good binding force and compatibility

Inactive Publication Date: 2009-10-14
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above method prepares nano-hydroxyapatite / collagen composite materials that meet certain performance requirements, the hydroxyapatite powder is easy to agglomerate, and it is actually difficult to disperse evenly and cannot form effective bonds with the collagen matrix. Ceramic particles It can only be loosely distributed in the collagen framework, and cannot effectively realize the biomimetic construction of nano-hydroxyapatite and collagen, thus limiting its function and application in the field of biomedicine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A preparation method of nano-hydroxyapatite / collagen scaffold with oriented particle arrangement comprises the following steps and process conditions thereof:

[0042] Step 1: Preparation of nano-hydroxyapatite sol

[0043] (1) Use polyvinylpyrrolidone (PVP K15) as template agent, dispersant and modifier at the same time;

[0044] (2) Use deionized water to prepare a solution containing 0.01mol / L of calcium nitrate and 0.006mol / L of diammonium hydrogen phosphate, and then add 0.1g of PVP K15 to the above calcium nitrate solution to fully dissolve;

[0045] (3) Using a high-speed disperser at 100 rpm, under the condition that the pH value of the ammonia water control system is 10.6, the materials in the above (1) and (2) are fully mixed to form a nano-hydroxyapatite sol, and the nano-hydroxyapatite sol is made. The molar ratio of calcium to phosphorus in the hydroxyapatite sol system reaches 1.6:1, and the content of polyvinylpyrrolidone is 0.01% by mass percentage;

...

Embodiment 2

[0056] A preparation method of nano-hydroxyapatite / collagen scaffold with oriented particle arrangement comprises the following steps and process conditions thereof:

[0057] Step 1: Preparation of nano-hydroxyapatite sol

[0058] (1) Use polyvinylpyrrolidone (PVP K30) as template agent, dispersant and modifier at the same time;

[0059] (2) Use the PVP K30 aqueous solution of mass percent 0.5% to prepare the solution containing calcium nitrate 0.1mol / L, disodium hydrogen phosphate 0.06mol / L;

[0060] (3) adopting a high-speed disperser of 1000 rpm, under the condition that the pH value of the sodium hydroxide control system is 11, the materials of the above-mentioned (1), (2) are fully mixed to form nano-hydroxyapatite sol, Make the molar ratio of calcium and phosphorus in the nano-hydroxyapatite sol system reach 1.65:1, and the content of polyvinylpyrrolidone by mass percentage is 0.5%;

[0061] Step 2: Aging of nano-hydroxyapatite sol

[0062] Move the above-mentioned hy...

Embodiment 3

[0071] A preparation method of nano-hydroxyapatite / collagen scaffold with oriented particle arrangement comprises the following steps and process conditions thereof:

[0072] Step 1: Preparation of nano-hydroxyapatite sol

[0073] (1) Use polyvinylpyrrolidone (PVP K60) as template agent, dispersant and modifier at the same time;

[0074] (2) use the PVP K60 aqueous solution of 5% by mass percentage to prepare the solution containing calcium nitrate 0.5mol / L, dipotassium hydrogen phosphate 0.3mol / L;

[0075] (3) adopt the high-speed disperser of 2000 revs / min, under the condition that potassium hydroxide control system pH value is 11.5, the material of above-mentioned (1), (2) is fully mixed, forms nano-hydroxyapatite sol, makes The molar ratio of calcium to phosphorus in the nano-hydroxyapatite sol system reaches 1.67:1, and the polyvinylpyrrolidone content is 5% by mass percentage;

[0076] Step 2: Aging of nano-hydroxyapatite sol

[0077] Move the above-mentioned hydroxya...

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Abstract

The invention discloses a method for preparing a nano hydroxyapatite/collagen scaffold with directionally arranged particles. The method takes polyvinylpyrrolidone as a template, a dispersant and a modifying agent simultaneously; the polyvinylpyrrolidone is fully mixed in an alkaline agent control system to form a sol system with the molar ratio of 1.6 to 1.7:1 between calcium and phosphorus and the content of 0.01 to 50 percent for the polyvinylpyrrolidone according to mass percent; the sol system is aged and washed, thus obtaining nano hydroxyapatite sol; then mixing and high-speed dispersion are conducted according to the proportion of 0.1 to 9:1 between the nano hydroxyapatite sol and a collagen solute according to mass ratio; and the mixture of the nano hydroxyapatite sol and the collagen solute is injected into a mould and refrigerated and dried, thus obtaining the nano hydroxyapatite/collagen scaffold. The hydroxyapatite particles in the scaffold are well dispersed in a collagenbasal body, form effective and stable linkage with collagens and are directionally arranged, thereby realizing a simulated structure of a natural bone from the view of the structure and the function.The method has simple technique, fast and convenient operation and easy promotion and application.

Description

technical field [0001] The invention relates to the technical field of artificial bone repair materials, in particular to a method for preparing nano-hydroxyapatite / collagen scaffolds with oriented particles. Background technique [0002] Bone defect repair has long been an important research topic in the field of clinical medicine and biomaterials at home and abroad. So far, the problem of repairing large-scale bone defects caused by trauma, infection and tumor resection has not been effectively solved clinically. Due to the limited source of autologous bone, it will bring pain to patients, while other bone repair materials, such as allogeneic bone, or xenogeneic bone may easily lead to immune problems and disease transmission. However, artificial bone repair materials still have many problems due to the large difference in highly precise mineralization structure from natural bone. [0003] As an artificial bone repair material, bionics in terms of composition and structu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/24A61L27/12
Inventor 王迎军宁成云尹兆益郑华德
Owner SOUTH CHINA UNIV OF TECH
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