The invention discloses a liquid formulation for trunk injection contained with extract of willow root for landscape plant insect attack and preparation and application thereof. The liquid formulation for trunk injection for landscape plant insect attack comprises the extract of the willow root, emamectin benzoate, surface active agent, anti-decomposing agent and cosolvent. The mass percents of the extract of the willow root, the emamectin benzoate, the surface active agent, the anti-decomposing agent and the cosolvent are 4 to 20%, 0.75 to 1.5%, 1 to 10%, 0.35 to 0.5% and 10 to 20% in sequence, and the margin is water. The liquid formulation for trunk injection for landscape plant insect attack is contained with the extract of the willow root, the insecticidal spectrum is wide, and the functional mechanism is unique, so that the drug resistance of the injurious insects can be solved effectively, features of contacting toxicity, stomach toxicity, systematic action and the like are possessed, drug application is conducted in an anhydrous and non-contact mode, the liquid formulation is safe to human and environment, and purposeful prevention and cure on the injurious insects like hemiptera, coleoptera, homoptera, diptera and some of lepidoptera at the places of forests, urban gardening, gardening trees in a scenic spot, border trees and protection forest are achieved.