Preparation method of inactivated porcine parvovirus vaccine and product thereof

Abstract

The invention discloses a preparation method of inactivated porcine parvovirus vaccine, comprising the following steps: (1) inoculating ST (swine testicle) cells with lentogen strains of porcine parvovirus, culturing to obtain production virus seeds; (2) adding treated microcarriers to a cell growth medium in a bioreactor, stirring; (3) inoculating the ST cells into the microcarriers in the bioreactor for cell reproduction and culturing; (4) infecting the suspension cultured ST cells in the bioreactor with the production virus seeds of the porcine parvovirus for virus reproduction and culturing; and (5) gathering the reproduced virus liquid, inactivating and preparing the vaccine. The method provided by the invention has long cell hold time, high cell viability and high density of living cells, and is favorable for continuous reproduction of virus, so that the prepared virus liquid has high virus titer. The production processes of the method disclosed by the invention are tightly and smoothly linked, the production scale is easy to enlarge, the porcine parvovirus is convenient to gather and stable in quality, and the method can obviously lower the production cost and effectively improve the yield and quality of the vaccine.

Description

Preparation method and product of porcine parvovirus inactivated vaccine technical field The invention relates to a preparation method of a veterinary live vaccine, in particular to a preparation method of an inactivated porcine parvovirus vaccine and an inactivated porcine parvovirus vaccine prepared by the method, belonging to the field of preparation of an inactivated porcine parvovirus vaccine. Background technique Porcine parvoviurs (PPV) can cause reproductive disorders in pigs, mainly manifested as infection and death of fetuses and embryos, while the mother usually does not show clinical symptoms. Serologically negative sows are primarily infected via the oronasal route during the first half of gestation, with consequent transplacental infection of immunocompromised fetuses leading to disease. The virus is ubiquitous in pig herds around the world and endemic in most farms. The disease is very popular and has been reported in many countries in Europe, America, Asia...

AI Technical Summary

Problems solved by technology

The technical problem to be solved by the present invention is to overcome the defects such as low porcine parvovirus liquid culture yield, difficult scale expansion, high cost and unstable vaccine quality in the existing preparation method of porcine parvovirus inactivated vaccine, and provide A new preparation method of porcine parvovirus inactivated vaccine. The preparation method utilizes microcarrier suspension culture ST cells in bioreactor to efficiently produce porcine parvovirus liquid. The production process parameters are optimized. The method has the advantages of porcine parvovirus produced The virus liquid has the advantages of high virus titer, large-scale industrial production, low cost, and stable vaccine quality.

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