Method for detecting suspension chip of multiple PCR products

A detection method and product technology, applied in the field of improvement of the conditions in the suspension chip detection method, can solve the problems of poor specificity, carcinogenicity, indistinguishable fragments of similar size, etc., and achieve the effect of flexible design
CN101560558AInactive Publication Date: 2009-10-21CHINESE ACAD OF INSPECTION & QUARANTINE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINESE ACAD OF INSPECTION & QUARANTINE
Publication Date
2009-10-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a non-diagnostic method for detecting a suspension chip of PCR products. The chip mainly comprises coded microspheres, a biotinylated primer, a capture probe, and streptavidin-biotin-phycoerythrin, and the method comprises the following steps that: the capture probe is coupled with corresponding microsphere of each size respectively, a red laser excites classified fluorescent lights on aspherical substrate, and the types are determined according to different colors of the spherical substrate, wherein the biotinylated primer shows the a primer needs biotinylation labeling during PCR; the microspheres coupled with the probe can be specifically combined with a PCR product labeled by an amplified biotin; and the streptavidin-biotin-phycoerythrin is combined with a biotin on the PCR product captured on the microspheres, a green laser excites the phycoerythrin, and the number of the reported fluorescent molecules combined on the spherical substrate is measured and is used for indirectly determining the content of the PCR product combined on the spherical substrate.
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Description

technical field

[0001] The invention provides a suspension chip detection method for multiple PCR products, and also relates to the improvement of conditions in the suspension chip detection method. Background technique

[0002] Polymerase chain reaction (PCR) is an in vitro amplification method that simulates natural DNA replication. The advent of PCR technology has brought a revolution to molecular diagnosis. PCR technology has become the basis of molecular diagnosis. The current PCR product detection method is agarose gel electrophoresis detection, and agarose gel can only distinguish DNA fragments with a difference of 100 bp. For PCR products with similar or equal fragment lengths, agarose gel electrophoresis will be powerless. In addition, agarose gel electrophoresis uses ultraviolet light to excite fluorescent dyes to generate fluorescence to determine whether there are PCR products, and the detection sensitivity is relatively low. Moreover, agarose gel electrophor...

Claims

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