Pyrophosphoric acid detection kit for common pathogenic bacteria and drug-resistant genomes as well as detection method and application of pyrophosphoric acid detection kit

Abstract

The invention discloses a pyrophosphoric acid detection kit for common pathogenic bacteria and drug-resistant genomes as well as a detection method and application of the pyrophosphoric acid detection kit. The kit is used for detecting 12 drug-resistant bacteria strains and drug-resistant genes in a sample to be detected, comprising genotypes of pseudomonas aeruginosa, acinetobacter baumannii, staphylococcus aureus, klebsiella pneumoniae, stenotrophomonas maltophilia, escherichia coli, enterococcus faecalis, enterococcus faecium, KPC gene, OXA-23 gene, SHV gene and MecA gene, and the kit comprises an amplification primer group, a sequencing primer group, an amplification solution, an amplification enzyme and a sequencing substrate. By adopting the pyrosequencing method, 12 pathogenic bacteria and drug-resistant genes with high pathogenicity and high detection rate can be detected at the same time, the pathogenic bacteria and the drug-resistant genes to be detected can be combined at will, detection of different frequencies and combinations can be carried out aiming at different environments, the detection process is flexible, simple and convenient, and the pyrosequencing result is rapid and readable.

Description

technical field [0001] The invention relates to a pyrophosphate detection kit for common pathogens and drug-resistant genomes, a detection method and an application thereof, and belongs to the field of gene detection. Background technique [0002] The Intensive Care Unit (ICU), also known as the comprehensive treatment room of the intensive care unit, provides isolation places and equipment for critically ill or comatose patients, providing the best nursing care, comprehensive treatment, combination of medical care and nursing, early postoperative rehabilitation, Joint care, exercise therapy, and other services. The ICU gathers critically ill patients and provides the best guarantee in terms of manpower, material resources and technology, in order to obtain a good treatment effect. If the hospital is the guarantee to defend people's life and health, then the ICU is the last line of defense for life. Therefore, once a pathogen infection occurs in the ICU, it will cause seri...

AI Technical Summary

Problems solved by technology

These methods have their own advantages and disadvantages. Plate culture is the gold standard for detection, but the culture cycle is long, the accuracy is low, and the operation is complicated; the chip has high throughput and fast detection, but has special requirements for the instrument. Real-time fluorescent PCR has high throughput and is easy to operate. , but the cost of the probe is high, which is not conducive to long-term use; the in situ hybridization method is involved early, but the detection cost is high and the throughput is low, and it is generally only suitable for the detection of gene amplification and deletion abnormalities

[0004] Sequencing methods include the first-generation, second-generation, and third-generation sequencing methods, as well as pyrosequencing methods. The first-generation sequencing method takes a long time to sequence and the cost of detection is high, while the second-generation and third-generation sequencing methods not only have high cost and long sequencing time, but also require professional third-party companies. The analysis results are not conducive to long-term clinical use

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