Drug resistance detection composition, drug resistance detection kit and drug resistance detection method

A technology for detection kits and detection methods, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve problems such as probe difficulties, achieve shortened detection time, simplify detection process, and high sensitivity and precision effects

Active Publication Date: 2022-04-05
SANSURE BIOTECH INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in specific applications, for specific two or even three drug-resistant genes, it is very difficult to find multiple primer pairs and corresponding probes that are compatible with each other and can perform specific amplification in the same reaction system. difficult

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  • Drug resistance detection composition, drug resistance detection kit and drug resistance detection method
  • Drug resistance detection composition, drug resistance detection kit and drug resistance detection method
  • Drug resistance detection composition, drug resistance detection kit and drug resistance detection method

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Embodiment Construction

[0030] In order to facilitate the understanding of the present invention, the following will describe the present invention more fully and give preferred embodiments of the present invention. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0031] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0032] The drug resistance detec...

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Abstract

The invention relates to a drug resistance detection composition, a drug resistance detection kit and a drug resistance detection method. The drug resistance detection composition includes a first pair of primers, a second pair of primers, a third pair of primers, a first probe needle, second probe and third probe. The invention provides a screening and optimized drug resistance detection composition, which can quickly and simultaneously detect three kinds of bacterial drug resistance genes by using multiple real-time fluorescent PCR technology, that is, carbapenem drug resistance genes KPC, class C The cephalosporin resistance gene CMY‑2 and the methicillin resistance gene mecA greatly simplify the detection process, shorten the detection time, and have high sensitivity and precision, providing more valuable nucleic acid samples with small quantities. The target detection information provides a simple and efficient detection method for scientific research and clinical bacterial resistance detection, which is of great significance to the success of clinical treatment and the rational application of antibiotics.

Description

technical field [0001] The invention relates to the field of molecular biology detection, in particular to a drug resistance detection composition, a drug resistance detection kit and a drug resistance detection method. Background technique [0002] The discovery and application of antibiotics, especially the clinical application of penicillin in the 1940s, ushered in a new era for humans to overcome infectious diseases, but the ensuing phenomenon of bacterial drug resistance posed a severe challenge to humans to conquer bacteria. In the past 20 years, a variety of new antibacterial drugs have been continuously used in clinics. Due to a large number of irrational use of antibiotics, bacterial drug resistance and multi-drug resistance have become increasingly serious, which has become a hot issue in the global medical community. More and more countries attach great importance to it. From the first discovery of penicillinase in Escherichia coli in 1940, the discovery of cepha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/686C12Q1/04C12N15/11
CPCC12Q1/686C12Q1/689C12Q2600/166C12Q2600/16C12Q2563/107C12Q2561/113
Inventor 孙青芝谭德勇邓中平刘佳戴立忠
Owner SANSURE BIOTECH INC
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