Reagent kit and method for quickly detecting staphylococcus MecA

A staphylococcus and kit technology, applied in the field of medicine and biology, can solve the problems of inability to meet bedside detection and critical value reporting, high cost, and wide application limitations, and achieve stable results, low operator requirements, and simple equipment.

Active Publication Date: 2019-07-19
WUHAN UNIV
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Problems solved by technology

Several DNA amplification-based real-time fluorescent quantitative PCR techniques have been used to detect the MecA gene of Staphylococcus sp. (Chung et al.2016; Nijhuis et al.2014; Tang et al.2014), but these methods require complicated DNA extraction process, special PCR amplification condi

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  • Reagent kit and method for quickly detecting staphylococcus MecA
  • Reagent kit and method for quickly detecting staphylococcus MecA
  • Reagent kit and method for quickly detecting staphylococcus MecA

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Embodiment 1

[0057] 1. Collection of specimens

[0058] 55 clinical isolates of Staphylococcus aureus (S.aureus), including 30 MRSA and 25 MSSA were used to establish this assay. 55 staphylococcus strains have been typed by PCR method, and the phenotype is consistent with the genotype. Another 293 clinical strains from Zhongnan Hospital of Wuhan University (n=229), Hainan Provincial General Hospital (n=30) and Tongji Hospital Affiliated to Huazhong University of Science and Technology (n=34) from 2017-2019 were used for retrospective analysis. Among them were 200 strains of Staphylococcus aureus and 56 strains of coagulase-negative Staphylococcus. Coagulase-negative staphylococci include Staphylococcus epidermidis (n = 23), Staphylococcus haemolyticus (n = 15), Staphylococcus lentus (n = 3), Staphylococcus worbachii (n = 3), Staphylococcus hominis subsp. =8), Staphylococcus capitis (n=4). Other types of 37 non-staphylococcal bacteria included Acinetobacter baumannii (n=8), Pseudomonas a...

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Abstract

The invention discloses a reagent kit and method for quickly detecting staphylococcus MecA. The reagent kit comprises cell lysate, amplifying reaction liquid, colloidal gold detection liquid and an immunochromatography reagent strip. The reagent kit is characterized in that transcription products of MecA and 16S rRNA are obtained through NASBA amplification on a primer pair in the amplifying reaction liquid. The reagent kit for quickly detecting genes of the staphylococcus MecA is stable in detection result, high in accuracy rate, high in sensitivity, low in cost and short in time consumptionand suitable for clinical promotion. The detection method is suitable for clinically and quickly detecting methicillin resistant staphylococcus and screening staphylococcus at pharynx nasalis, is quick and accurate, is simple in equipment, is low in requirements for operators, and has potential in bedside detection and application in hospitals at different levels.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a kit and method for rapidly detecting Staphylococcus MecA. Background technique [0002] Staphylococci, including Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis), are widely distributed in the human population and in the natural environment. They are normal colonizers on the skin and mucous membranes of humans and animals and can be obtained from Soil, water, milk, food, etc. (Miragaia 2018) (Huijbers et al. 2015). Although staphylococci only exist as colonizing bacteria in most cases and do not cause harm to the body, when the host is immunocompromised or the skin and mucous membrane barriers are damaged, their invasion potential will appear, resulting in Hospital or community-acquired infection (Natsis and Cohen 2018; Poulakou et al. 2019). [0003] Antibiotic resistance is a threat to the prevention and treatment of bacterial infections. (Mi...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6806C12Q1/14C12Q1/6865C12R1/44
CPCC12Q1/6806C12Q1/6865C12Q1/689C12Q2565/514C12Q2563/116C12Q2531/113
Inventor 李一荣娄宁叶光明周俊英方芳赵瑾李雪寒曾晓娇熊梦园付宇杨京冬姜湘湖刘奕
Owner WUHAN UNIV
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