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Bionic digestion determination method for total quantity of digestible carbohydrates of feed

A technology that can digest carbon and hydrates. It is applied in the direction of color/spectral characteristic measurement. It can solve the problems of system error, result repeatability and poor precision, so as to eliminate system error, improve repeatability and precision, and reduce workload. Effect

Active Publication Date: 2016-11-16
HUNAN ZHONGBEN INTELLIGENT TECH DEVCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the in vitro evaluation technology of dietary carbohydrates has attracted more and more attention internationally, which has promoted the rapid development of related research. However, most of these in vitro methods are based on simple experimental equipment such as triangular flasks, test tubes, and dialysis tubes. Systematic error introduced by manual operation, resulting in poor repeatability and accuracy of results

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  • Bionic digestion determination method for total quantity of digestible carbohydrates of feed
  • Bionic digestion determination method for total quantity of digestible carbohydrates of feed
  • Bionic digestion determination method for total quantity of digestible carbohydrates of feed

Examples

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Embodiment 1

[0044] refer to figure 1 A biomimetic digestion method for measuring the total amount of digestible carbohydrates in a pig feed of the present embodiment, comprising the following steps:

[0045] 1) Sample preparation: Sampling is carried out according to GB / T 14699.1, and then the sampled pig feed sample is divided into quarters to about 200g, and the sample is crushed with a plant grinder or mortar until it passes through a 60-mesh sieve (aperture 0.30mm) , sealed in a sample bag and stored as a sample for later use;

[0046] 2) Preparation of buffers and simulated digestive solutions and other reagents for the stomach and small intestine:

[0047] Stomach buffer solution (pH 3.0): Weigh 2.59g sodium chloride, 0.25g potassium chloride and 1g potassium sorbate, put them into a 500mL beaker, add 400mL deionized water to dissolve, and add 2mol / L hydrochloric acid (HCl) in The pH of the solution was adjusted to 3.0 at 39°C. After cooling, transfer the above solution into a 50...

Embodiment 2

[0062] refer to figure 1 , a kind of biomimetic digestion determination method of feed digestible carbohydrate total amount of the present embodiment, has the following difference with embodiment 1:

[0063] In step 1), the feed sample is chicken feed.

[0064] In step 2), the preparation method of the gastric buffer solution: Weigh 0.5425g sodium chloride, 0.3925g potassium chloride and 1.5g potassium sorbate, put them into a 500mL beaker, add 400mL deionized water to dissolve, and use 2mol / L of hydrochloric acid (HCL) at 41 ° C to adjust the pH of the solution to 3.0, after cooling, transfer the above solution to a 500 mL volumetric flask, and dilute to volume with deionized water.

[0065] In step 2), the preparation method of the gastric simulated digestive juice: according to the pepsin activity assay method described by Wirnt R, the pepsin activity in pepsin was measured. Then, according to the concentration of pepsin in chicken simulated gastric juice is 1550U / ml, we...

Embodiment 3

[0072] refer to figure 1 , a kind of biomimetic digestion determination method of feed digestible carbohydrate total amount of the present embodiment, has the following difference with embodiment 2:

[0073] In step 1), the feed sample is duck feed.

[0074] In step 2), the preparation method of the gastric simulated digestive juice: according to the pepsin activity assay method described by Wirnt R, the pepsin activity in pepsin was measured. Then, according to the concentration of pepsin in duck simulated gastric juice is 1550U / ml, weigh 387.5KU of pepsin and dissolve it in 250ml of hydrochloric acid solution with pH 2.0 (pH calibration at 42°C). Stir slowly until dissolved, do not heat simulated gastric juice on a heating plate, or overheat during preparation; prepare just before use.

[0075] The preparation method of the small intestine buffer solution: weigh 0.6067g of anhydrous disodium hydrogen phosphate and 2.4867g of anhydrous sodium dihydrogen phosphate.

[0076]...

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Abstract

A bionic digestion determination method for the total quantity of digestible carbohydrates of feed includes the following steps that 1, sample preparation is carried out; 2, a buffer solution and simulated digestive juice of the stomach and the small intestine and other reagents are prepared; 3, a monogastric animal bionic digestive system is preheated; 4, sample loading is carried out; 5, stomach simulated digestion treatment is carried out; 6, small intestine simulated digestion treatment is carried out, wherein small intestine simulated digestion parameters are set through monogastric animal bionic digestive system control software, and small intestine simulated digestion treatment is started; 7, digestion residue treatment is carried out; 8, the content of reducing sugar in a control sample and the content of reducing sugar in an analysis sample are determined with the DNS colorimetric method, and the total quantity of digestible carbohydrates of the feed is worked out. According to the bionic digestion determination method for the total quantity of digestible carbohydrates of the feed, the external digestion process is automatically controlled in the monogastric animal bionic digestive system, and the test result is high in reproducibility and precision; the workload of operators is reduced, system errors caused by manual operation are eliminated, and the detection efficiency is high.

Description

technical field [0001] The invention relates to a method for measuring digestible carbohydrates, in particular to a bionic digestion method for determining the total amount of digestible carbohydrates in feed. Background technique [0002] Carbohydrates are composed of three elements: carbon, hydrogen and oxygen. Carbohydrates in feed can be gradually degraded into glucose, fructose, semi Monosaccharides such as lactose and a small amount of disaccharides containing free aldehyde groups (lactose, maltose). [0003] Objective and accurate evaluation of the biological efficacy of various feed ingredients is the basis for the study of animal nutritional requirements and optimization of feed formula, and is the basis for decision-making to improve the utilization rate of livestock and poultry diets, reduce the cost of diets, and reduce energy conservation and emission reduction in aquaculture. The evaluation methods of carbohydrate biological potency in feed mainly include in v...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 邓耀辉李敏陈开宇彭建伟
Owner HUNAN ZHONGBEN INTELLIGENT TECH DEVCO
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