Bionic evaluation method of available phosphorus in pig feed

A technology for pig feed and bionic digestion, which is applied in the direction of analysis by chemical reaction of materials, analysis of materials, and material analysis by observing the influence of chemical indicators, which can solve the problems of poor repeatability and accuracy of results, and achieve The effect of improving reproducibility and accuracy and eliminating systematic errors

Active Publication Date: 2013-01-30
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

However, most of these in vitro methods are based on simple experimental equipment such as triangular flasks, test tubes, and dialysis tub

Method used

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  • Bionic evaluation method of available phosphorus in pig feed
  • Bionic evaluation method of available phosphorus in pig feed
  • Bionic evaluation method of available phosphorus in pig feed

Examples

Experimental program
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Embodiment Construction

[0025] like figure 1 Shown, concrete operation method of the present invention can be implemented in the following manner:

[0026] (1) Cut the dialysis bag into small pieces of about 25 cm, and boil the dialysis bag for 10 minutes in a solution of 2% sodium bicarbonate and 1 mmol / L disodium edetate at pH=8.0. After thoroughly washing the dialysis bag with distilled water, place the dialysis bag in a solution of 1 mmol / L disodium ethylenediaminetetraacetate at pH = 8.0 and boil for 10 minutes. Store the solution at 4°C after cooling. Rinse the dialysis bag thoroughly with deionized water before use.

[0027] (2) Preparation of gastric and small intestinal buffer and simulated digestive fluid.

[0028] (21) Stomach buffer: 85mmol / L sodium chloride and 6mmol / L potassium chloride solution with a pH of 2.0.

[0029] (22) Small intestine buffer: 190mmol / L sodium acetate, 97mmol / L sodium chloride and 14mmol / L potassium chloride solution at pH 6.3.

[0030] (23) Simulated gastri...

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Abstract

A bionic evaluation method of available phosphorus in pig feed. A monogastric animal bionic digestion system is utilized to determine in vitro digestibility of phosphorus in feed. The method comprises the steps of dialysate bag pretreatment, preparation of a gastrointestinal tract simulated digestive juice and a buffer solution, computer operation and determination of effective phosphorus. The method comprises the following specific steps of: weighing a feed sample and placing to a simulation digester with dialysis bag; adding the buffer solution and the simulated digestive juice; setting digestion parameters; simulating the process of digestion; and after the digestion, determining phosphorus content by a molybdenum blue colorimetric method. According to the invention, real-time dynamic digestion product separation technology is utilized, and in vitro digestion process is automatically controlled by the monogastric animal bionic digestive system, so as to improve reproducibility and accuracy of the test results.

Description

technical field [0001] The invention relates to a method for bionic evaluation of available phosphorus in pig feed, in particular to a method for measuring available phosphorus after simulating the phosphorus in feed being digested and absorbed in the gastrointestinal tract of pigs under conditions in vitro of animals. Background technique [0002] Phosphorus is an essential mineral element in animals and plants, and is the third most expensive feed material after protein and energy (Lei et al., 2000; Novak et al., 2000; Fan et al., 2001; Huo Qiguang, 2002). Objective and accurate evaluation of the biological efficacy of various feed ingredients is the basis for the study of animal nutritional requirements and optimization of feed formula, and is the basis for decision-making to improve the utilization rate of livestock and poultry diets, reduce the cost of diets, and reduce energy conservation and emission reduction in aquaculture. The evaluation methods of feed phosphorus ...

Claims

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Application Information

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IPC IPC(8): G01N33/00G01N21/78
Inventor 张宏福陈亮庄晓峰李东卫赵峰
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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