In vitro culture method of cancer type organs

An in vitro culture and organ technology, applied in the field of cell engineering, can solve the problems of low enzymatic hydrolysis efficiency, easy contamination of samples, and inability to subculture for a long time, so as to improve enzymatic hydrolysis efficiency and maintain cell viability
CN110656086AActive Publication Date: 2020-01-07NANOPEPTIDE QINGDAO BIOTECH LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANOPEPTIDE QINGDAO BIOTECH LTD
Publication Date
2020-01-07

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Abstract

The invention provides an in vitro culture method of cancer type organs. According to the embodiment of the invention, the method comprises the steps of performing shearing and digestion treatment oncancer tissue samples, wherein the size of cancer tissue after the shearing treatment is 1-2mm<3>, and the digestion treatment is performed under the action of combined digestive enzymes; and performing re-suspension treatment on cancer cells obtained after digestion treatment with precooling substrate glue, wherein the concentration of the cancer cells in the substrate glue is 500-1500 / 10 [mu]L;and performing point and pallet treatment on cancer cell suspension, and performing culture treatment on the cancer cells after point and pallet treatment is performed, so as to obtain cancer organs.According to the method of the embodiment of the invention, the enzymolysis efficiency of the cancer cells is improved, the success rate of subculturing of organoids is increased, and long-term culture of the organoids can be realized, and a biology sample database can be established.
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Description

technical field

[0001] The present invention relates to the field of cell engineering, more specifically, the present invention relates to a method for culturing cancerous organoids in vitro. Background technique

[0002] Colon adenocarcinoma is one of the main malignant tumors in humans, threatening human health. The study of its pathogenesis found that the abnormality of WNT, RAS-MAPK, PI3K, TP53, TGF and other signaling pathways plays a key role in the occurrence of colon adenocarcinoma. In addition, with the further in-depth study of colon adenocarcinoma genome, it is found that patients with colorectal cancer have microsatellite DNA instability or chromosomal instability. Compared with other cancers, colon adenocarcinoma has more genetic abnormalities and diversity, and patients often Having multiple gene mutations or multiple gene abnormalities. Therefore, it is impossible to evaluate the general efficacy of the treatment plan for patients only through the analysis o...

Claims

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