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Method for clathrating tributyrin by enzyme method

A technology for combining tributyrin and tributyrin, which is applied in the field of enzymatic inclusion of tributyrin, can solve the problems of poor fluidity, low oil content, poor solubility, etc. The effect of storage period and quality assurance

Active Publication Date: 2018-10-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although tributyrin has better potential application value than butyric acid and its salts, it also has some problems of peculiar smell and poor fluidity. The special inner cavity of cyclodextrin is used to clathrate tributyric acid Glyceride, which can effectively improve its dispersion and flowability, mask odor and extend shelf life
If cyclodextrin products are directly used as wall materials to clathrate tributyrin, there are disadvantages such as high cost, low oil content, poor solubility, etc., and the production process of cyclodextrin has not been reported yet.

Method used

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  • Method for clathrating tributyrin by enzyme method
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  • Method for clathrating tributyrin by enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Comparison of cyclodextrin inclusion tributyrin and enzymatic method inclusion tributyrin

[0031] The process of directly inclusion tributyrin with cyclodextrin is as follows:

[0032] Glyceryl tributyrate is mixed with ethanol in advance (mass ratio of glyceryl tributyrate and ethanol is 1:1), 4% β-cyclodextrin is heated and dissolved, and after cooling to 50°C, the ethanol solution of tributyrin is slowly It is added to the above solution, wherein the mass ratio of tributyrin added to β-cyclodextrin is (0.1-0.5):1. Thoroughly shake and mix, and sonicate at 50°C for 30min. After the reaction is finished, freeze-drying is carried out to obtain dryness and sealed storage.

[0033] The preparation process of enzymatic inclusion tributyrin is carried out as follows:

[0034] (1) Using cornstarch solution with a mass concentration of 20% as a substrate, add CGTase to liquefy at 90°C for 60 minutes, wherein the amount of CGTase added is 2U / (g starch wet basis);...

Embodiment 2

[0044] Example 2 Preparation of tributyrin embedding material with different substrates

[0045] The preparation process of tributyrin powder oil is carried out as follows:

[0046] (1) Using cornstarch, potato starch or maltodextrin (DE=10) with a mass concentration of 20% as substrates, add 2U / (g starch or maltodextrin wet basis) CGTase to liquefy at 90°C for 60min;

[0047] (2) Cool to 45°C, add 2U / (g starch or malt paste wet base) CGT enzyme to carry out cyclization reaction for 12 hours, after the end, add tributyrin, tributyrin and starch or malt paste to the system The ratio of fine to raw material is 0.30:1 (mass ratio);

[0048] (3) Add Tween-20 emulsifier to the above solution and mix well. The ratio of emulsifier to starch or maltodextrin is 0.2:1 (mass ratio)

[0049] (4) High-speed shearing machine 8000~2000rpm under high-speed shearing above-mentioned solution 2~5min;

[0050] (5) Homogenize the emulsion obtained in (4) to obtain a uniform and stable emulsion,...

Embodiment 3

[0052] Example 3 Effect of the amount of liquefaction enzyme added on the emulsion during the preparation of powder tributyrin

[0053] The preparation process of tributyrin powder oil is carried out as follows:

[0054] (1) Using 20% ​​corn starch as a substrate, add CGT enzyme to liquefy at 90°C for 60 minutes, wherein the amount of liquefied enzyme added is 1, 1.5, 2, 3, 4 U / (g starch wet basis);

[0055] (2) Cool to 45°C, add 2U / (g starch) CGT enzyme to carry out cyclization reaction for 12h, add tributyrin to the system after completion, the ratio of tributyrin to starch raw material is 0.30:1 (mass Compare);

[0056] (3) Add Tween-20 emulsifier to the above solution and mix evenly, the ratio of emulsifier to starch is 0.2:1 (mass ratio);

[0057] (4) High-speed shearing machine 8000~2000rpm under high-speed shearing above-mentioned solution 2~5min;

[0058] (5) Homogenize the emulsion obtained in (4) to obtain a uniform and stable emulsion, the homogenization pressure...

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Abstract

The invention discloses a method for clathrating tributyrin by an enzyme method, and belongs to the technical field of grease microencapsulation. According to the method, cyclodextrin synthesized by the enzyme method and tributyrin clathrated by cyclodextrin are combined. The method comprises the step of preparing the cyclodextrin by the enzyme method using CGT enzymes; the tributyrin is added inthe preparation process; after the reaction, Tween is added; and homogenizing and spray drying are performed. The effect of the finally obtained tributyrin powder is much better than the single cyclodextrin clathration effect. The method provided by the invention has the advantages that the process is simple; the cost is low; the operation is convenient; non-toxic harmless and pollution-free effects are achieved in the reaction process; no toxic reagent is remained; the clathration effect is obvious; and the better utilization of a nutritive additive of the tributyrin in the practical production is facilitated.

Description

technical field [0001] The invention relates to a method for enzymatic inclusion of tributyrin, which belongs to the technical field of oil microencapsulation. Background technique [0002] Tributyrin (tributyrin, C 15 h 26 o 6 ), which is esterified from three molecules of butyric acid and one molecule of glycerol. Among them, butyric acid, as a kind of short-chain fatty acid, is considered to be a potential substitute for antibiotic feed additives due to its environmental protection, safety in use, improvement of animal production performance, and maintenance of intestinal structural integrity. There are many problems in the process, such as easy dissociation, easy volatility, special odor, and short half-life. Tributyrin, as the precursor of butyric acid, has a long half-life, is convenient to use, is safe and has no toxic side effects, which not only solves the problem that butyric acid is volatile and difficult to add in a liquid state, but also improves the problem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12P19/18A23K20/158A23K40/30
CPCA23K20/158A23K40/30C12P19/04C12P19/18A61K31/19A23K20/163A23K10/14A23V2002/00A23K50/75A23K50/10A23K50/30A61K47/6951A23V2200/32A23V2250/1946A23V2250/5112A61K9/107
Inventor 李才明李兆丰陈双娣顾正彪冯焱李泽西程力洪雁
Owner JIANGNAN UNIV
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