27 results about "Glyceryl tributyrate" patented technology

The invention discloses the use of tributyrin as an additive for embryo in vitro development culture medium, and belongs to the technical field of animal reproductive biology. The invention applies tributyrin as an additive to mouse embryo in vitro development culture medium for the first time, and the tributyrin can significantly improve the blastocyst rate, reduce the ROS content in the embryo, improve the mitochondrial membrane potential in the embryo, improve the ATP level in the embryo and the expression of antioxidant genes, improve the DNA methylation and histone modification level in the embryo, and promote the embryo in vitro development; and tributyrin, as a natural antioxidant and epidrug, is safe and has no toxic side effects, is cheap and easy to obtain, does not need to replace the culture medium during the entire development and culture process, and is simple to operate; the tributyrin provides strong support for in vitro embryo engineering technologies such as the efficient development of embryos in human assisted reproductive technology, mammalian fertilized embryos, parthenogenetic embryos and somatic cell cloned embryos, and has good application prospects.

The present invention relates to the use of an animal feed additive composition for controlling the growth of undesirable microorganisms, said composition comprising glycerides, vegetable oils, tannins and / or saponins, the glycerol ester is selected from the group consisting of glycerol monopropionate, glycerol dipropionate and glycerol tripropionate, glycerol monobutyrate, glycerol dibutyrate and glycerol tributyrate, glycerol monovalerate, glycerol divalerate and glycerol trivalerate, and mixtures thereof. The invention also relates to the use of the animal feed additive composition for promoting intestinal growth, alleviating or alleviating effects from necrotizing enteritis, modulating intestinal flora and / or as an additive to drinking water.

The application discloses a Pseudomonas atacamensis STR8 and an application thereof, and belongs to the technical field of microorganisms. The strain is classified and named as Pseudomonas atacamensis STR8, and the preservation number is CCTCC NO: M 2025609. The hydrolysis circle HC value of the strain on a tributyrin plate is 2.78, and the lipase activity reaches 139.86 U / mL. The application also provides an application of the strain and a lipase produced by the strain in catalyzing hydrolysis of tocopherol succinate to prepare tocopherol. The strain STR8 is fermented to obtain a crude enzyme liquid, and the crude enzyme liquid is added into a reaction system containing the tocopherol succinate to perform a hydrolysis reaction. After 9 hours of reaction, the content of the tocopherol is increased by 750% compared with a control group. The strain and the method provided by the application have high catalytic efficiency, mild reaction conditions and environmental friendliness, and are suitable for green industrialized production of the tocopherol.

The present application relates to a kind of fat powder for reducing weaning piglet dead rate and its preparation method and application, and the composite lipid in the preparation raw material of fat powder includes 1,3-di-oleic acid-2-palmitic acid triglyceride, 24 degrees palm oil, rice bran oil, coconut oil, linseed oil, caprylic triglyceride, capric acid triglyceride, alpha-lauric acid monoglyceride and tributyrin.The present application develops the fat acid composition close to pig milk fat structure, let piglet continue lactation period in weaning period to enable approach mainly with lipid, meet the compound energy demand of fast, medium and slow, avoid falling into energy potential difference;And the specific combination of medium and short chain fatty acids, both can improve the energy efficiency of weaning piglet, also have bacteriostatic disease characteristics, can improve the immunity of piglet, reduce the diarrhea rate.This composite lipid overcomes the problem such as piglet energy deficiency and indigestion caused by single raw material oil due to unsuitable lipid structure, can significantly reduce weaning stress, reduce the diarrhea rate and dead rate.

The compositions and methods for improving or regulating GLP-1 levels, stimulating GLP-1 secretion, ameliorating metabolism including losing weight, managing glucose or insulin tolerance including stimulating insulin secretion, inhibiting glucagon secretion and reducing fasting glucose levels. The compositions comprise β-aminoisobutyric acid, a physiologically acceptable salt, polymer, ester, or 3-hydroxy-3-methylbutanoic acid, a physiologically acceptable salt, polymer, ester, or tributyrin or tributyrin derivative, or mixture thereof.

The present invention discloses a system and method for effectively promoting esterification reaction, including a first reaction kettle, a second reaction kettle, a dryer, a first heat exchanger, a second heat exchanger, a mixing tank, and a transfer pump. The method includes: (1) Butyric acid or methanol enters the second reaction kettle for heat exchange through the second heat exchanger and the first heat exchanger; (2) Glycerol or fatty acid is added to the first reaction kettle, and a catalyst and butyric acid or methanol are added to the mixing tank and dissolved and mixed; (3) The glycerol or fatty acid in the first reaction kettle is mixed with the water-containing butyric acid or methanol in the first heat exchanger and the catalyst in the mixing tank to carry out the first esterification reaction; (4) The glycerol tributyrate or methyl ester in the first reaction kettle is pumped into the second reaction kettle, and anhydrous butyric acid or anhydrous methanol is added through the first heat exchanger for mixing to carry out the second esterification reaction; (5) High-purity glycerol tributyrate or biodiesel is discharged from the second reaction kettle. The present invention adopts a two-stage or multi-stage reaction kettle for reaction, can continuously feed, continuously react, and continuously discharge materials, with low cost and high efficiency.

The invention discloses an application of butyric acid or a derivative thereof in preparation of a zearalenone reproductive toxicity resisting preparation. Also disclosed is an antitoxic feed composition comprising a specific base ration and a butyric acid derivative. In-vivo and in-vitro tests and molecular mechanism researches prove that butyric acid and derivatives thereof (such as tributyrin and sodium butyrate) can effectively relieve zearalenone (ZEN)-induced animal ovary injury. The action mechanism of the traditional Chinese medicine composition is that follicular atresia is reduced, the balance of reproductive hormones (such as GnRH, E2, AMH and P4) is recovered, and clinical symptoms caused by ZEN poisoning such as vulva swelling and the like are relieved by activating a BMPs / SMAD signal channel, especially up-regulating SMAD4 protein expression and inhibiting oxidative stress (ROS) and Caspase-3 mediated granular cell apoptosis.

The invention discloses a lightweight heat-insulating diatomite thermal insulation board and a preparation method thereof, and relates to the technical field of thermal insulation boards. The preparation method comprises the following steps: dissolving cellulose gum in water to form a solution; mixing diatomite powder, silica aerogel powder, metal oxide nanoparticles, aluminum hydroxide, tributyrin and calcium carbonate, gradually adding microencapsulated phase change material and continuing to stir, and then adding cellulose gum solution, stirring and mixing; putting the mixture into a mold, compacting and drying to obtain a board; coating the board with silica aerogel solution and curing. The present invention significantly enhances the thermal regulation performance and thermal insulation capacity of the thermal insulation board by using microencapsulated phase change material and silica aerogel, and achieves low thermal conductivity and excellent thermal comfort. The use of lightweight diatomite and aerogel materials reduces the weight of the product and improves the thermal insulation performance of the structure.

An animal feed additive composition comprising: a) 45-65 % by weight, with respect to the total weight of the a)+b), of a butyric acid glycerol ester, said butyric acid glycerol ester comprising at least 75 % by weight of tributyrin, and b) 35-55% by weight, with respect to the total weight of the a)+b), of a valeric acid glycerol ester, said valeric acid glycerol ester comprising at least 30 % by weight of monovalerin.

This invention discloses a strain of *Enterobacter cloacae* EC11 and its applications, belonging to the field of microbial technology. The strain is classified as *Enterobacter cloacae* EC11, with the accession number CCTCC NO:M 2025610. This strain exhibits a hydrolysis zone HC value of 3 on tributyrate agar plates and a lipase activity of 145.28 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting the lipase strain EC11 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 725% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.

The invention discloses Korean pseudomonas STR7 and application thereof, and belongs to the technical field of microorganisms. The strain is classified and named as Pseudomonas koreensis STR7, and the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M 2025608. The HC value of a hydrolysis circle of the strain on a tributyrin plate is 3, and the lipase activity reaches 136.81 U / mL. The invention also provides application of the strain and the lipase produced by the strain in preparation of tocopherol by catalyzing hydrolysis of tocopherol succinate. The strain STR7 is fermented to obtain crude enzyme liquid, the crude enzyme liquid is added into a reaction system containing tocopherol succinate for hydrolysis reaction, and after the reaction is performed for 9 hours, the content of tocopherol is increased by 865% compared with that of a control group. The strain and the method provided by the invention are high in catalytic efficiency, mild in reaction condition, environment-friendly and suitable for green industrial production of tocopherol.

The invention provides a tributyrin solid preparation, a preparation method thereof and a feed additive containing the solid preparation, and belongs to the technical field of feed additive.The tributyrin and PLGA are dissolved in acetone and injected into a crystallization kettle through a nozzle under the supercritical CO2 condition, and a solvent is rapidly extracted to form high-encapsulation-efficiency nanometer microspheres; carrying out high-speed shearing and high-pressure homogenization treatment on the microspheres and a water phase containing maltodextrin, hydroxypropyl-beta-cyclodextrin, Arabic gum and the like to form a submicron emulsion; and finally, performing spray drying to obtain spherical microcapsule powder, and tabletting or filling capsules. The solid preparation comprises tributyrin, PLGA (poly (lactic-co-glycolic acid)), a viscoelastic matrix and a plurality of functional auxiliary materials, has the characteristics of high encapsulation efficiency, uniform particle size, good stability and low water content, and is compounded with a protein carrier, a carbohydrate carrier and the like as a core component; the prepared feed additive can effectively improve the processing stability, palatability and bioavailability of tributyrin.

The invention relates to an oral pharmaceutical composition and a preparation method thereof. The oral pharmaceutical composition comprises: (a) taxane; (b) at least one medium chain triglyceride selected from the group consisting of glyceryl triacetate, glyceryl tributyrate, glyceryl trihexanoate, glyceryl tricaprylate, glyceryl tricaprylate, and glyceryl trioleate; (c) a glyceryl oleate complex comprising glyceryl monooleate, glyceryl dioleate, and glyceryl trioleate, the content of the glyceryl monooleate being 30 wt% to 99.9 wt%; (d) cholesterol, a cholesterol derivative, or a combination thereof; and (e) a surfactant.

This invention discloses a strain of *Pseudomonas fluorescens* STR2 and its applications, belonging to the field of microbial technology. The strain is classified as *Pseudomonas fluorescens* STR2, with accession number CCTCC NO: M 2025606. This strain exhibits a hydrolysis zone HC value of 4.23 on tributyrate plates and a lipase activity of 149.72 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting strain STR2 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 700% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.

The present application relates to the field of fertilizers, and specifically discloses an organic polypeptide enzyme activity promoter and a preparation method thereof; an organic polypeptide enzyme activity promoter, comprising the following raw materials in parts by weight: animal brain, animal pancreas, soybean, soybean shell, peanut, loaded porous filler, and adhesion promoter; the loaded porous filler is prepared by loading calcium carbonate micropowder and tributyrin in sequence after hydrophobic modification of a porous filler; the preparation method thereof is as follows: weighing the animal pancreas, chopping it, mixing it with part of the adhesion promoter, then adding the loaded porous filler and mixing it evenly, and treating it to obtain a primary mixture; weighing the animal brain, soybean, and peanut, chopping them, mixing them evenly, and obtaining a mixture; mixing the primary mixture evenly with soybean shell, then adding the mixture and the remaining adhesion promoter, mixing evenly, and granulating to obtain a finished product; after the application of the organic polypeptide enzyme activity promoter, the crop is not easily affected by drought in the early growth stage, and is not easily affected by floods in the later growth stage, thereby obtaining a higher yield.

The invention provides short-chain fatty acid ester as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing short-chain fatty acid, glycerol and a catalyst, and reacting under heating and stirring; after the reaction is finished, taking out reaction liquid, and removing the catalyst to obtain a short-chain fatty acid ester crude product; and separating to obtain a short-chain fatty acid ester pure product. The short-chain fatty acid is butyric acid or propionic acid, and the obtained short-chain fatty acid ester is tributyrin or glyceryl tripropionate. The method uses the acidic ionic liquid or solid acid as the catalyst to catalyze the reaction of butyric acid or propionic acid and glycerol under certain conditions to synthesize tributyrin and glycerol tripropionate, has the advantages of high conversion rate, short time consumption, simple process operation, easy separation, environmental protection and the like, and is good in safety, high in yield, low in cost and suitable for industrial production. The industrial production of the tributyrin and the glyceryl tripropionate is facilitated. The short-chain fatty acid ester is applied to preparation of food, medicine or feed for relieving colitis, and the relieving effect on colitis is good.

The present application belongs to the technical field of feed additive, and particularly relates to a compound fatty acid preparation for promoting chicken growth, and a preparation method and application thereof. The compound fatty acid preparation for promoting chicken growth is mainly composed of the following raw materials in parts by weight: 3-6 parts of stigmasterol, 2-4 parts of campesterol, 10-20 parts of linoleic acid, 12-24 parts of tributyrin, 1-2 parts of emulsifier, 3-5 parts of soybean phospholipid, 3-5 parts of protective agent, and 30-80 parts of water. The combination of stigmasterol and campesterol can significantly improve the targeted release efficiency of linoleic acid and tributyrin. The released butyric acid can activate the mitochondrial beta-oxidation pathway of intestinal epithelial cells, significantly improve the efficiency of tricarboxylic acid cycle, and enhance the intestinal barrier function. The phytosterol can also competitively inhibit the NPC1L1 transporter, reduce the intestinal cholesterol absorption, direct the energy to the growth demand, thereby improving the daily weight gain of broilers and reducing the feed-meat ratio.

Compositions and methods for improving or regulating GLP-1 levels, or increasing GLP-1 receptors, or reducing GLP-1 degradation, or stimulating GLP-1 secretion in a subject. The composition comprises two or more of the following substances: an effective amount of dihydroberberine (DHB) or berberine, a physiologically acceptable salt, polymer, ester, acid thereof; gymnema sylvestre or gymnemic acid; cinnamon or ceylon cinnamon; nobiletin; or the composition comprises an effective amount of dihydroberberine (DHB), a physiologically acceptable salt, polymer, ester, acid, and one or more of the following substances: β-aminoisobutyric acid, a physiologically acceptable salt, ester; 3-hydroxy-3-methylbutanoic acid, a physiologically acceptable salt, ester; tetrahydrocurcumin, a physiologically acceptable salt, acid, ester; tributyrin; berberine; resveratrol; rhodiola rosea; cinnamon; fenugreek; olive leaf extract; grain of paradise; fruit-vegetable powder; whey protein; or arginine.

The present application relates to the technical field of pharmaceutical preparation, in particular to a nanoemulsion containing tamoxifen and ginsenoside and a preparation method thereof. The present application prepares the nanoemulsion by citric acid tamoxifen, ginsenoside, glyceryl tributyrate, isopropyl myristate, tragacanth, poloxamer and glycerol, the prepared nanoemulsion has smaller particle size, good stability, can significantly improve the solubility, and further improve the targeting and therapeutic effect of the nanoemulsion.

The invention discloses a method for accurately determining the activity of swine-derived co-lipase, which comprises the following steps: emulsifying tributyrin by using gum arabic powder and glycerol together, and performing enzymolysis on the tributyrin into free fatty acid according to the coenzyme action of co-lipase and the combined action of co-lipase and lipase, thereby obtaining the activity of the swine-derived co-lipase. Meanwhile, the blank that the tributyrin is independently enzymolyzed by the same amount of lipase is taken, finally, the activity of the co-lipase is calculated according to the calculation formula by utilizing the volume difference value of the sodium hydroxide solutions consumed for generating the free fatty acid under the two conditions, and the method for designing the activity of the pig-derived co-lipase is stable and accurate in detection result.

The invention discloses a feed additive based on lysolecithin and butyrate compounding and a preparation method thereof, and belongs to the technical field of feed additives. A preparation method of the feed additive based on lysolecithin and butyrate compounding comprises the following steps: firstly, mixing zinc nitrate and deionized water to obtain a solution A; 2-methylimidazole and deionized water are mixed, tributyrin, neutral protease and xylanase are added, and a solution B is obtained; pouring the solution A into the solution B, stirring and reacting, collecting a precipitate, and washing and drying to obtain drug-loaded ZIF-8 powder; dissolving lysolecithin in a solvent to obtain a coating solution, spraying the coating solution on the surface of the drug-loaded ZIF-8 powder, and drying to obtain the drug-loaded ZIF-8 powder. The feed additive based on lysolecithin and butyrate compounding can improve the emulsification efficiency and absorption and utilization rate of feed fat, reduce liver lipid deposition, reduce transaminase activity, improve liver and gall health and strengthen intestinal functions.

This invention discloses a strain of Bacillus wiedmannii STR18 and its applications, belonging to the field of microbial technology. The strain is classified as Bacillus wiedmannii STR18, with accession number CCTCC NO:M 2025611. This strain exhibits a hydrolysis zone HC value of 3 on tributyrate plates and a lipase activity of 140 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting strain STR18 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 800% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.

The invention discloses a preparation method and application of a tributyrin catalyst, and belongs to the field of tributyrin preparation. The catalyst used in the method is a tantalum-containing compound or an acid-modified tantalum-containing compound, and compared with a traditional method for preparing tributyrin through a protonic acid catalyst, the catalyst is high in activity, capable of achieving the yield of tributyrin of 90% or above, environmentally friendly, not prone to causing equipment corrosion and free of generating a large amount of reaction waste; meanwhile, the catalyst is easy to prepare and low in cost, the product and the catalyst can be separated for recycling through simple filtering operation, the catalytic performance of the catalyst cannot be reduced after recycling, and the yield of 90% or above can still be kept.

This invention discloses a strain of *Enterobacter cloacae* STR5 and its applications, belonging to the field of microbial technology. The strain is classified as *Enterobacter cloacae* STR5, with accession number CCTCC NO: M 2025607. This strain exhibits a hydrolysis zone HC value of 3.95 on tributyrate plates and a lipase activity of 121.53 U / mL. This invention also provides the application of this strain and its produced lipase in the catalytic hydrolysis of tocopherol succinate to prepare tocopherol. The crude enzyme solution obtained by fermenting strain STR5 was added to a reaction system containing tocopherol succinate for hydrolysis. After 9 hours of reaction, the tocopherol content was increased by 700% compared to the control group. The strain and method provided by this invention have high catalytic efficiency, mild reaction conditions, and are environmentally friendly, making them suitable for the green industrial production of tocopherol.

The invention discloses a feed additive containing tributyrin and a preparation method thereof. The feed additive is prepared by the following steps: dispersing microspheres in a mixed solvent, adding tetraethoxysilane for reaction to obtain a product, adding an etching agent for stirring reaction to obtain HMSNs, dispersing the HMSNs in a solvent, adding a coupling agent for reaction to obtain aminated HMSNs, dissolving carboxymethyl chitosan and an activating agent in a buffer solution, adding a coupling agent for reaction to obtain aminated HMSNs, and performing ultrasonic dispersion to obtain the feed additive. The preparation method comprises the following steps: adding aminated HMSNs to react to obtain HMSNs modified by CMCS on the inner wall, dispersing the HMSNs modified by CMCS on the inner wall in a solvent, adding succinic anhydride to carry out ring-opening reaction, adding ethanolamine to stir to obtain a product, dispersing the product in a solvent, adding a catalyst and palmitoyl chloride to obtain HMSNs modified by CMCS on the inner wall and palmitate on the outer wall, and carrying out reaction on the HMSNs modified by CMCS and palmitate on the outer wall. And finally, mixing with an oil phase formed by mixing tributyrin and glycerol monocaprylate. The feed additive provided by the invention can improve the utilization rate and the acting effect of active ingredients.

The application provides a tributyrin solid preparation and a preparation method thereof, and a feed additive containing the solid preparation, and belongs to the technical field of feed additives.The tributyrin and PLGA are dissolved in acetone, injected into a crystallization kettle through a nozzle under the condition of supercritical CO2, and the solvent is quickly extracted to form high-encapsulation-rate nanometer microspheres; the microspheres and an aqueous phase containing maltodextrin, hydroxypropyl-beta-cyclodextrin, gum arabic and the like are subjected to high-speed shearing and high-pressure homogenization treatment to form a submicron emulsion; finally, spherical microcapsule powder is prepared through spray drying, and the powder can be tabletted or filled into capsules.The solid preparation contains tributyrin, PLGA, a viscoelastic matrix and various functional excipients, has the characteristics of high encapsulation rate, uniform particle size, good stability and low water content, and when the solid preparation is used as a core component and is compounded with a protein carrier and a carbohydrate carrier, the prepared feed additive can effectively improve the processing stability, palatability and bioavailability of the tributyrin.

The invention provides application of triglyceride as an extracting agent and a using method of the triglyceride, and belongs to the technical field of application of triglyceride. The invention provides an application of triglyceride as an extracting agent, the triglyceride has higher solubility and selectivity, can effectively improve the extraction efficiency, has the characteristics of no toxicity and biodegradability, is environment-friendly, and is easy to recycle and reuse. Meanwhile, the invention also provides a method for extracting the vanillin, which comprises the following steps: mixing the vanillin with the triglyceride, and extracting to obtain the vanillin. According to the method, the vanillin is extracted by utilizing the triglyceride butyrate, so that a vanillin product with the purity of 98% or above is obtained.