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Method for extracting active alkaloid from lycoris herb

A technology of Lycoris plants and active alkaloids, which is applied in the direction of fermentation, etc., can solve the problems of low utilization rate of Lycoris resources, low product yield, and difficult industrialization, and achieve low cost, increased value, and high production efficiency. Effects in simple steps

Inactive Publication Date: 2011-04-20
HUAZHONG UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In a word, the existing method for producing alkaloids with Lycoris as raw material has the following deficiencies: single product, cumbersome steps, long cycle, low product yield, low safety factor, high cost, low resource utilization rate of Lycoris and environmental pollution. Serious pollution, and difficult to industrialize

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) extraction

[0030] Take 10kg of dry Lycoris flakes and 30kg of aqueous solution in a multifunctional extraction tank, adjust the pH between 6-7, and keep the temperature at room temperature, add 13.5g of cellulase, 9.6g of pectinase, and 6.9g of hemicellulase, Enzymolysis for 8 hours, after the end of the enzymolysis, use mechanical crushing technology to mash the plant tissue, filter, and concentrate the filtrate at a vacuum of 0.08-0.09MPa and 70°C to obtain 1.3L of extract with a specific gravity of 1.10 (at 20°C) Concentrate.

[0031] The mechanical disruption technique can be tissue mash homogenization technique, solid sample crushing technique, high pressure cell disruption technique or flash extraction technique.

[0032] (2) Extraction

[0033] The first step of extraction: adjust the pH of the concentrated extract to 6.5, add diethyl ether for 2 extractions, the dosage is 1.3L and 0.65L respectively, combine the ether phases to recover the diethyl ether ...

Embodiment 2

[0041] (1) extraction

[0042]Take 10kg of dried Lycoris flakes and 40kg of aqueous solution in a multifunctional extraction tank, adjust the pH between 5-6, and the temperature is 40°C, add 8.4g of cellulase, 6.6g of pectinase, and 5g of hemicellulase, After 2 hours of enzymatic hydrolysis, the sample was mashed and homogenized by tissue, filtered, and the filtrate was concentrated at a vacuum of 0.08-0.09 MPa at 77°C to obtain 1.2L of an extract concentrate with a specific gravity of 1.07 (at 20°C).

[0043] (2) Extraction

[0044] The first step of extraction: adjust the pH of the extract concentrate to 5.0, add n-butanol to carry out three extractions, the dosage is 0.6L, 0.4L, 0.4L respectively, combine the n-butanol phase, and recover n-butanol under reduced pressure to obtain The first step extracts the product;

[0045] The second step of extraction: the pH value of the water layer extracted in the first step is adjusted to 9.0-9.5, the third step of chloroform is ex...

Embodiment 3

[0052] (1) extraction

[0053] Take 10kg of dry Hudixiao tablets, 100kg of aqueous solution, adjust the pH between 7-8, and the temperature is 55 degrees, add 21.15g of cellulase, 13.95g of pectinase, 9.9g of hemicellulase, enzymolysis for 24 hours, use The high-shear flash extractor crushed the sample for 10 minutes, filtered, and the filtrate was concentrated at 77° C. at a vacuum of 0.08-0.09 MPa to obtain 2.1 L of extract concentrate with a specific gravity of 1.05 (at 20° C.).

[0054] (2) Extraction

[0055] The first step of extraction: adjust the pH of the concentrated extract to 6.0, add ethyl acetate for 4 extractions, each time with an amount of 0.6 L, combine the ethyl acetate phase, and recover the ethyl acetate under reduced pressure to obtain the first step extraction product;

[0056] The second step of extraction: adjust the pH value of the water phase extracted in the first step to 9.3-9.8, extract twice with chloroform, the dosage is 0.8L and 0.6L, combine ...

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Abstract

The invention discloses a method for extracting active alkaloid from lycoris plants. The method utilizes the combination of an enzymic method and modern mechanical wall-breaking technique to extract lycoris alkaloid. Lycoris alkaloid is extracted efficiently in excellent yields under mild conditions, other impurities dissolution is lowered and the consistency of extract is decreased for continuing treatment of isolation and purification. Segmental extraction technique is adopted according to the alkalinity of lycoris alkaloid to get a plurality of raw products of lycoris alkaloid. Then highlypurified monomer of lycoris alkaloids (galanthamin, galanthidine, dihydrogalanthamine, sekisamine) are available after column chromatography and repeated recrystallization. The invention has the advantages of simple line, no pollution, short cycle, high yield of products, high safety coefficient, low cost, diversity of outputs, thus raising the comprehensive utilization of lycoris and reducing the manufacturing cost of deep processing and increasing the feasibility of industrial production.

Description

technical field [0001] The invention belongs to the field of natural organic chemistry and relates to a method for extracting active alkaloids from Lycoris plants. Background technique [0002] Lycoris Herb. is an important genus in the family Amaryllidaceae, which is a perennial herb. According to incomplete statistics, there are about 20 species in the world, mainly distributed in the warm temperate to subtropical regions of East Asia. Lycoris contains a lot of alkaloids. Foreign scientists extracted and isolated a large number of alkaloids in the early 20th century. Such as Lycorine (lycorine), tazettine (polyflora narcissine), homolycorine (homolycorine), lycoramine (lycoramine), lycorine (lycorenine), pseudolycorine (pseudlycorine), norpre Alkaloids such as norpluviine, epigalanthamine, vittatine, pluviine, and hippeastrine. [0003] my country's science and technology is relatively backward, and the research on Lycoris alkaloids was not carried out until the 1950s,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/18
Inventor 敖明章余龙江廖娜崔永明江喜敏张明
Owner HUAZHONG UNIV OF SCI & TECH
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