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Method of screening silky character of chicken by SNP (Single Nucleotide Polymorphism) detection

A technology for silk feather and traits, which is applied in the field of SNP detection and screening of chickens, can solve the problems of lack of molecular marker methods for detecting chicken silk feather traits, and achieve the effects of improving breeding efficiency, high accuracy, and low cost

Active Publication Date: 2014-03-26
CHINA AGRI UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] At present, the interaction between Shh (Sonic hedgehog) and Bmp2 (Bone morphogenetic protein2) signaling pathways has been confirmed to be related to the formation of barbs (Harris, M.P., S.Williamson, J.F.Fallon, et al. Molecular evidence for an activator-inhibitor mechanism in development of embryonic feather branching.Proc Natl Acad Sci U S A,2005,102(33):11734-11739), among them, but there are few studies on the development and differentiation of the pinnae at the molecular level, There is even a lack of molecular markers for the detection of silk feather traits in chickens

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  • Method of screening silky character of chicken by SNP (Single Nucleotide Polymorphism) detection
  • Method of screening silky character of chicken by SNP (Single Nucleotide Polymorphism) detection
  • Method of screening silky character of chicken by SNP (Single Nucleotide Polymorphism) detection

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Embodiment 1

[0033] Embodiment 1 Establishment of the method of the present invention

[0034] 1. Genotype analysis

[0035] (1) Experimental materials

[0036]The CAU resource family is an experimental group established in 1998 by the research group of Professor Li Ning of China Agricultural University to locate genes that affect important economic traits of chickens. The parents are French star broilers and Taihe silky chickens (Deng, X.M., Li, J.Y. , Li, N., and Wu, C.X.(2001). Genetic analysis of important growth trait based on F-2 resource population in chicken. Yi Chuan Xue Bao 28, 801-807). This experiment selected the F of 4 families of CAU resource families 0 , F 1 and F 2 Generation individuals, a total of 278.

[0037] (2) Extraction of genomic DNA

[0038] When the chicken was 12 weeks old, blood was collected from the wing vein, lysed after anticoagulant treatment, digested with proteinase K, extracted with phenolic form, dissolved in TE and stored at -20°C. For details,...

Embodiment 2

[0058] Embodiment 2 Method of the present invention detects the silk feather character of chicken

[0059] 4 breeds (a total of 267 individuals) with a silk feather phenotype and 28 populations or breeds (a total of 307 individuals) with a flake feather phenotype were determined, and the populations or breeds to be tested were obtained from the Poultry Research Institute of the Chinese Academy of Agricultural Sciences Institute and China Agricultural University, see Table 2 for specific varieties.

[0060] 1. Genomic DNA extraction

[0061] When the chicken was 12 weeks old, blood was collected from the wing vein, lysed after anticoagulant treatment, digested with proteinase K, extracted with phenolic form, dissolved in TE and stored at -20°C. For details, please refer to "Analysis and Study on Mapping of Important Phenotypic Traits and Candidate Genes of Chickens" (Gao Yu, [Ph.D. Dissertation], 2006, Beijing: China Agricultural University).

[0062] 2. PCR amplification

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Abstract

The invention discloses a method of screening silky character of chicken by SNP (Single Nucleotide Polymorphism) detection. The method comprises the following steps: 1) extracting a DNA (Deoxyribonucleic Acid) sample of chicken to be detected, and carrying out PCR (Polymerase Chain Reaction) amplification by using DNA specific primer pairs to obtain a PCR amplification product; and 2) carrying out SNP detection on the third chromosome 70486623bp site of chicken in the PCR amplification product. The invention provides an efficient, accurate, simple and quick chicken breeding assistant selection molecular genetic mark and provides an efficient molecular mark breeding means for seed selection and retention of silky character of chicken. The detection method disclosed by the invention is simple to operate, low in cost and high in accuracy, and can realize automatic detection.

Description

technical field [0001] The invention relates to a method for SNP detection and screening of chickens, in particular to a method for SNP detection and screening of silk feather traits of chickens. Background technique [0002] Single nucleotide polymorphisms (Single Nucleotide Polymorphisms, SNP) refer to the variation of a single nucleotide in the genome, which can be stably inherited to offspring during the transmission of genetic material, so it can be used as a molecular marker for breeding and conservation. [0003] Appearance is one of the important breed characteristics of chicken (Gallus gallus domesticus), which plays an important role in breeding and conservation. At present, there are still few studies on molecular markers controlling chicken appearance characteristics, and only a small number of appearance characteristics can be passed through molecular markers. markers for assisted selection breeding. [0004] Feather is one of the most complex epidermal appenda...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A01K67/02
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 胡晓湘冯春刚宋迟顾晓荣王彦强李宁
Owner CHINA AGRI UNIV
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