Molecular genetic maker for egg quality of laying hen and application of molecular genetic marker

A technology of molecular genetic markers and genetic markers, which is applied in the field of molecular genetic marker-assisted selection of laying hens, can solve the problems of lower egg production, achieve the effects of increased selection intensity, low cost, and accelerated genetic progress

Inactive Publication Date: 2017-05-24
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

A base mutation (G/C) in the coding region of the VLDLR gene leads to a mutation of cysteine ​​(Cys) somewhere on the protein chain to ser...

Method used

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  • Molecular genetic maker for egg quality of laying hen and application of molecular genetic marker
  • Molecular genetic maker for egg quality of laying hen and application of molecular genetic marker
  • Molecular genetic maker for egg quality of laying hen and application of molecular genetic marker

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Embodiment

[0022] 1. Design primers

[0023] According to the DNA sequence of the chicken VLDLR gene (accession number: NC_006127) and the DNA sequence of the CTSD gene (accession number: NC_006092) on GenBank, the following primers were designed (Table 1).

[0024] Table 1 Chicken VLDLR and CTSD gene polymorphism detection primers

[0025]

[0026]

[0027] 2. PCR amplification

[0028] The PCR reaction system was 20 μL, in which 1×Buffer (containing 3mM Mg 2+ ) 2.0μL, 2mM dNTPs 2.0μL, 0.5pmol / L upstream and downstream primers 2.0μL each, template DNA 50ng, 1U Taq DNA polymerase 0.2μL, add ddH 2 0 to 20.0 μL. PCR amplification reaction program: pre-denaturation at 95°C for 2min; denaturation at 94°C for 30s, annealing at 59°C for 1min and 30s, extension at 72°C for 1min, 40 cycles; finally extension at 72°C for 10min. The amplification effect of the PCR product was detected by 3% agarose gel electrophoresis, and sent to a sequencing company for sequencing.

[0029] 3. PCR pro...

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Abstract

The invention belongs to the technical field of laying hen molecular genetic marker assisted selecting, and particularly relates to a molecular genetic marker for egg quality of a laying hen and application of the molecular genetic marker. The molecular genetic marker is a partial sequence of genes VLDLR and CTSD of the laying hen, and a VLDLR nucleotide sequence is as shown in SEQ ID No.1, and VLDLR nucleotide sequences are as shown in SEQ ID No.2-4. The molecular making method is utilized for selecting egg quality characters, so that a molecular marking method which is more effective, simpler and more convenient to implement is provided for marker assisted selecting in chicken breeding work; meanwhile, the molecular genetic marker can overcome the defects that the normal egg quality character selecting method is great in workload, is easily affected by the environment, is long in breeding period and the like, and provides an effective molecular marker breeding means for egg quality character improving, so that genetic progress of chicken is quickened.

Description

technical field [0001] The invention belongs to the technical field of molecular genetic marker-assisted selection of laying hens, and in particular relates to a molecular genetic marker of egg quality of laying hens and its application. Background technique [0002] Egg quality directly affects the nutritional content and edible value of the egg, and then affects the market price of the egg. At the same time, it has a certain impact on the storage time of the egg, the hatching rate of the hatching egg, and the breakage rate. Egg quality is largely affected by genetic factors. The heritability is usually between 0.5 and 0.7, and the heritability of egg weight and eggshell strength is relatively high. Breed is the most important factor affecting the quality of conventional eggs. Genetic improvement of egg quality is an important direction for laying hen breeding in the future, and it is also an extremely important indicator in laying hen production. [0003] In recent years,...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 卢立志李国勤田勇陈黎沈军达陶争荣曾涛徐坚
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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