Method for screening novel restoring gene for HL cytoplasm male sterile rice

A technology for restoring genes and male sterility, which is applied in the fields of plant genetic improvement, botany equipment and methods, biochemical equipment and methods, etc. It can solve the problem that there is no additive effect of strong and weak restorer genes, and the BT and WA types cannot be restored at the same time Male sterility and other problems, to achieve the effect of increasing genetic diversity, improving breeding efficiency, and improving screening efficiency

Inactive Publication Date: 2010-12-01
WUHAN UNIV
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Problems solved by technology

Moreover, the same restorer gene cannot restore BT-type and WA-type male sterility at the same time. Only after recombination and combining Rf1 and Rf3 in the same restorer line can they re...

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  • Method for screening novel restoring gene for HL cytoplasm male sterile rice
  • Method for screening novel restoring gene for HL cytoplasm male sterile rice
  • Method for screening novel restoring gene for HL cytoplasm male sterile rice

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Embodiment Construction

[0041] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of protection of the present invention. In the following examples, specific experimental conditions and methods are not indicated, usually according to conventional conditions such as: editors such as J. Sam Brook, Science Press, 1992, Molecular Cloning Experiment Guide (Second Edition); D.L. Spector et al., Science Press, 2001, Cell Experiment Guide; Lu Hongsheng, Science Press, 1982, or follow the conditions suggested by the manufacturer. Specific steps are as follows:

[0042] A method for screening novel restorer genes of red lotus-type cytoplasmic male sterile rice, the steps of which are:

[0043] 1. PCR primer design:

[0044] The primers were designed based on the co-segregated molecular markers of rice red lotus-type cytoplasmic restoration...

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Abstract

The invention discloses a method for screening a novel restoring gene for HL cytoplasm male sterile rice. The method comprises the following steps of: firstly, designing a primer of a restoring gene coseparation molecular marker and establishing a standard polymerase chain reaction (PCR) amplification system; secondly, comparing and analyzing a molecular marker amplification banding pattern and screening a rice variety or an ecotype having different molecular markers; and finally, verifying a new restoring gene locus by using genetic analysis and determining the novel restoring gene. In the method, the conventional genetic analysis is combined with molecular genetic marker technology, and a new gene resource is developed and utilized purposefully. The method has the characteristics of simple and convenient operation, rapidness, correctness and accurate selection of a novel restorer and is favorable for expanding restoring spectrum, improving the polymerization selection efficiency of excellent recovering genes and promoting the maturing rate, yield and sustainable development of hybrid rice.

Description

technical field [0001] The present invention relates to the technical field of molecular marker detection, and more specifically to a method for rapid and qualitative detection of red lotus-type cytoplasm, which is applicable to rice red lotus-type gametoplasmic male sterility in various rice cultivars, farm species and wild rice Screening for fertility restorer genes. Background technique [0002] Plants have accumulated a large amount of variation in the genome through natural mutation or recombination during the long evolutionary process, resulting in differences among different strains or strains within the same species. This difference is manifested at the genetic level as the difference of certain DNA segments from each other. When this variation is co-segregated with a specific gene, it can be used as a unique molecular signature of the gene. If these DNA fragments are subjected to in vitro amplification, electrophoresis, and color development, bands of different si...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A01H1/02
Inventor 李绍清谭艳平谢红卫
Owner WUHAN UNIV
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