Method for extracting active ingredient of folium artemisiae argyi and application of active ingredient in resistance of plant disease

A technology for active ingredients and plant diseases, applied in the field of botanical pesticides, can solve problems such as economic losses in agricultural production, short duration of effect, and loss of efficacy of copper preparations

Inactive Publication Date: 2014-06-11
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Like viral diseases, plant bacterial diseases have the characteristics of high frequency of occurrence, serious disasters, wide range of occurrence, difficulty in prevention and control, and severe damage. They have caused huge economic losses to agricultural production and seriously affected my country's food and economy. crop production safety
At present, the pesticides used to prevent and control plant bacteria in the market are mainly copper preparations, such as thiabyl copper, can kill, etc., but copper preparations are easy to be washed by rain and lose their efficacy, the duration of effect is too short, and the control effect is poor.

Method used

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  • Method for extracting active ingredient of folium artemisiae argyi and application of active ingredient in resistance of plant disease
  • Method for extracting active ingredient of folium artemisiae argyi and application of active ingredient in resistance of plant disease
  • Method for extracting active ingredient of folium artemisiae argyi and application of active ingredient in resistance of plant disease

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, the extraction method of the active ingredient of Artemisia annua is carried out according to the following steps successively:

[0028] (1) Heat and reflux 20kg of dried and crushed Artemisia annua in 85% industrial ethanol for 2 times, each time for 2.5 hours. After filtering, heat and reflux the filter residue with 70% industrial ethanol for 2 hours and filter. Combined, and then concentrated under reduced pressure to remove alcohol to obtain ethanol extract.

[0029] (2) Add a small amount of distilled water to the crude ethanol extract, disperse it in an ultrasonic instrument, then extract it with petroleum ether, chloroform, ethyl acetate, and n-butanol in sequence, and concentrate under reduced pressure to remove the solvent to obtain a petroleum ether layer extract, chloroform layer extract, ethyl acetate layer extract, n-butanol layer extract and aqueous layer extract.

[0030] (3) Dissolve the above-mentioned chloroform layer extract in absolute...

Embodiment 2

[0040] Compound 8 α -Hydroxyartemisinin Anti-cucumber mosaic virus activity test (half-leaf blight method).

[0041] Anti-cucumber mosaic virus activity test test is carried out in the following steps:

[0042] (1) Virus purification

[0043] Using the Gooding method (Gooding G V jr, Hebert T T.A simple technique for purification of tobacco mosaic virus in large quantities [J]. Phytopathology, 1967, 57, 1285.), select the inoculation for more than 3 weeks, and the CMV system infects the host Nicotianatabacum. L The upper leaves of the plant were homogenized in phosphate buffer, then homogenized with 10% chloroform:n-butanol (1:1), filtered through double-layer gauze, and centrifuged at 1000g to obtain the crude extraction liquid of CMV. The whole experiment was carried out at 4°C (Junichi Fukami, Yasuhiko Uesugi [Japanese], Li Shuzheng, Wang Duku, Jiao Shumei translated. Experimental Methods of Pesticides - Fungicides [M] Beijing: Agricultural Press, 1991, 93-94 .). Measur...

Embodiment 3

[0055] Compound 8 α -Hydroxyartemisinin Anti-tobacco mosaic virus activity test (half-leaf blight method).

[0056] Anti-tobacco mosaic virus activity test test is carried out in the following steps:

[0057] (1) Virus purification:

[0058] Using the method of Gooding et al. (Gooding G. V. jr; Hebert T T., A simple technique for purification of tobacco mosaic virus in large quantities [J]. Phytopathology , 1967 , 57(11): 1285-1290.), select inoculation for more than 3 weeks, TMV system infects the upper leaves of the host Nicotiana tabacum. Treat with polyethylene glycol, centrifuge again, and suspend the precipitate with phosphate buffer to obtain the crude extraction of TMV. The whole experiment was carried out at 4°C (Junichi Fukami, Yasuhiko Uesugi [Japanese], translated by Li Shuzheng, Wang Duku, Jiao Shumei. Pesticide Experimental Methods - Fungicides [M]. Beijing: Agricultural Press , 1991 , 93-94.). Measure the absorbance value at 260nm wavelength with an ult...

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Abstract

The invention provides a preparation method of an active substance capable of inhibiting plant viruses and plant bacteria and application of the active substance. The preparation method of the active substance capable of inhibiting plant viruses and plant bacteria comprises that folium artemisiae argyi in Guizhou province is taken as raw material, dried in the sun and then smashed, ethanol extraction is carried out; ethanol extract is extracted by virtue of trichloromethane; reduced pressure concentration is carried out on a trichloromethane extraction layer, so that crude extract is obtained; silica column chromatography is carried out on trichloromethane crude extract, gradient elution is carried out by virtue of petroleum ether-acetic acid ethyl ester mixed eluant in different ratios, then purification means like the silica column chromatography is carried out for a plurality of times, and two monomer compounds, namely 8 alpha-hydroxyl artemisia terpene and 5,4'-dihydroxyl-6,7,3'-trimethoxy flavone, are obtained. Biological activity tests show that the monomer compound I has good prevention and control effect on plant viruses such as cucumber mosaic virus, tobacco mosaic virus, south rice black-streaked dwarf virus and rice stripe virus and 5,4'-dihydroxyl-6,7,3'-trimethoxy flavone has good inhibiting effect on bacterial disease pathogenic bacteria such as tomato bacterial wilt, tobacco bacterial wilt, rice bacterial leaf blight and rice bacterial leaf streak.

Description

technical field [0001] The invention belongs to the technical field of botanical pesticides, and relates to a preparation method and application of an active substance capable of inhibiting plant diseases. Specifically, it uses a plant produced in Guizhou as a raw material, and is extracted by various chemical means and methods. Among the active substances, the active substances have a high inhibitory effect on plant viruses and bacterial pathogens. Background technique [0002] Plant virus is a kind of plant disease with great harm and difficult to control. It is called "plant cancer". Once it occurs, it is difficult to control. Cucumber mosaic virus disease, tobacco mosaic virus disease, southern rice black-streaked dwarf virus disease and rice stripe leaf blight virus are the main virus diseases in the production of grain and economic crops. Not even harvested. According to statistics, the damage caused by cucumber mosaic virus disease, tobacco mosaic virus disease, sou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/10C07D311/30C07D311/40A01N43/90A01N43/16A01P1/00
CPCA01N43/16A01N43/90C07D311/30C07D311/40C07D493/10
Inventor 宋宝安韩宇胡德禹薛伟万治华陈美航金林红贺鸣陈卓李向阳
Owner GUIZHOU UNIV
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