Molecular marker of paddy rice major gene site capable of preventing bacterial leaf streak and applications thereof
A molecular marker and resistance gene technology, applied in the field of molecular genetics, can solve the problem of different major gene loci of bacterial leaf spot resistance, and achieve the effects of convenient identification, clear location and clear selection target.
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[0016] 1. Screening of molecular markers
[0017] 1. Materials and methods
[0018] 1.1 Test materials
[0019] The susceptible material 9311 is a well-known rice restorer line, and the disease-resistant material DY19 is a source of resistance to common wild rice obtained through repeated inoculation, re-screening and verification.
[0020] 1.2 Method
[0021] 1.2.1 Genetic analysis of resistance genes
[0022] DY19 was crossed with 9311, inoculated to observe the phenotype of F1 plants, and the seeds on F2 single plants were harvested by selfing, and the F2 population was planted. After inoculation, the lesions were counted separately for genetic analysis of bacterial stripe disease.
[0023] 1.2.2 Construction of molecular marker population
[0024] The susceptible female parent 9311 was crossed with the resistant male parent DY19 to obtain F1, and self-crossed to obtain F2 population. Heterozygous genotype plants were selected by genetic testing, and 9311 was used as t...
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