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Molecular marker of paddy rice major gene site capable of preventing bacterial leaf streak and applications thereof

A molecular marker and resistance gene technology, applied in the field of molecular genetics, can solve the problem of different major gene loci of bacterial leaf spot resistance, and achieve the effects of convenient identification, clear location and clear selection target.

Active Publication Date: 2018-04-10
GUANGXI UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Most of the above studies have obtained the corresponding molecular markers, which are different from the main effect gene loci of bacterial streak resistance obtained in this study

Method used

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  • Molecular marker of paddy rice major gene site capable of preventing bacterial leaf streak and applications thereof
  • Molecular marker of paddy rice major gene site capable of preventing bacterial leaf streak and applications thereof
  • Molecular marker of paddy rice major gene site capable of preventing bacterial leaf streak and applications thereof

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] 1. Screening of molecular markers

[0017] 1. Materials and methods

[0018] 1.1 Test materials

[0019] The susceptible material 9311 is a well-known rice restorer line, and the disease-resistant material DY19 is a source of resistance to common wild rice obtained through repeated inoculation, re-screening and verification.

[0020] 1.2 Method

[0021] 1.2.1 Genetic analysis of resistance genes

[0022] DY19 was crossed with 9311, inoculated to observe the phenotype of F1 plants, and the seeds on F2 single plants were harvested by selfing, and the F2 population was planted. After inoculation, the lesions were counted separately for genetic analysis of bacterial stripe disease.

[0023] 1.2.2 Construction of molecular marker population

[0024] The susceptible female parent 9311 was crossed with the resistant male parent DY19 to obtain F1, and self-crossed to obtain F2 population. Heterozygous genotype plants were selected by genetic testing, and 9311 was used as t...

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Abstract

The invention discloses a molecular marker of a paddy rice major gene site capable of preventing bacterial leaf streak and a primer pair for amplifying the molecular marker. The inventor subjects Guangxi wild rice resistant resources DY19(male) and 9311(female) to hybridization, backcross, and selfing to obtain BC3F2 offspring; and then resistance identification and molecular genetics linkage analysis are performed to obtain a molecular marker SL41, which is in close linkage with a gene capable of preventing bacterial leaf streak. The location of the major gene site detected by developed molecular marker is definite, the identification is convenient, and the molecular marker has an important meaning for the long term control of bacterial leaf streak. Moreover, the operation of using the molecular marker to detect bacterial leaf streak of a breeding material is simple, the accuracy can reach 95% or more, the production cost is saved, the efficiency of screening paddy rice species capable of preventing bacterial leaf streak is largely improved, the breeding cycle of disease resistant paddy rice species is shortened greatly, and the breeding efficiency is improved.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and in particular relates to a molecular marker and application of a major gene locus for resistance to bacterial spot disease of rice. Background technique [0002] Rice bacterial streak disease (the pathogen is Xanthomonas oryzae pv.oryzicola (Fang, Ren, Chu, Faan, wu) Swings called Xanthomonas oryzae pv.oryzicola (Fang, Ren, Chu, Faan, wu) Swings called Xanthomonas oryzae pv. disease, streak disease, mainly damages leaves. The lesions of rice bacterial streak disease are small dark green water-soaked spots at first, and soon expand into dark green to yellowish-brown thin streaks between the veins, with a size of about 1×50mm, and the two ends of the lesions are infiltrating green. A large amount of beaded yellow bacterial pus often overflows from the lesions, which are small colloidal particles after drying. When the disease is severe, the streaks merge into irregular yellowish-brown to wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 李容柏施力军刘芳罗登杰覃宝祥
Owner GUANGXI UNIV
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