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SNP marker location for rice bacterial leaf streak resistant gene BLS2 and application of SNP marker location

A kind of thin stripe disease and gene technology, applied in the field of molecular biology, can solve the problems of less research on molecular genetic mechanism and in-depth research on molecular basis, and achieve the effect of improving selection efficiency

Inactive Publication Date: 2017-08-18
广西壮族自治区农业科学院水稻研究所
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AI Technical Summary

Problems solved by technology

Most of the studies on the main effect genes of resistance to thin stripe disease are concentrated on the screening of resistance sources and the analysis of resistance genetics, while there are few studies on the molecular genetic mechanism of disease resistance genes, especially the molecular basis research such as the positioning of related genes is not yet in-depth.

Method used

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  • SNP marker location for rice bacterial leaf streak resistant gene BLS2 and application of SNP marker location
  • SNP marker location for rice bacterial leaf streak resistant gene BLS2 and application of SNP marker location
  • SNP marker location for rice bacterial leaf streak resistant gene BLS2 and application of SNP marker location

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Embodiment 1

[0037] 1. Construction of Multiparent Advanced Generation Inter-Cross (MAGIC)

[0038] Before constructing the population, the resistance identification of the three parents was carried out, and the results were as follows: O. Susceptible 998R hybridization, F1 self-crossing for 10 consecutive generations, obtained Multiparent Advanced Generation Inter-Cross (MAGIC) S10, and carried out resistance identification on the obtained multi-parent Advanced Generation Inter-Cross (MAGIC) population, Obtain 30 extremely disease-resistant individual plants and mix them to construct a disease-resistant pool (N915) for sequencing; obtain 30 extremely susceptible individual plants and mix them to construct a disease-resistant pool (N908) for sequencing.

[0039] 2. Identification of resistance

[0040] At the tillering stage of rice, the representative strain JZ28 (type Ⅲ) of dominant pathogenic bacterial leaf spot widely distributed in Guangxi was used for inoculation. After the strain ...

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Abstract

The invention relates to the technical field of biology and particularly relates to an SNP marker location for rice bacterial leaf streak resistant gene BLS2 and an application of the SNP marker location. Gene BLS2 is located through a single nucleotide polymorphism marker on a chromosome 6, and the sequence is as shown in SEQ ID NO:1. The marker location comprises the specific steps of construction of multi-parent advanced intercross line groups from generation to generation; genomic sequencing; bioinformation analysis; screening of parental homozygous difference SNP sites; frequency analysis on two BSA pool genes according to position information of screened homozygous SNP sites with difference between parents to obtain the sites related to the disease-resistant gene. The gene can be applied to breeding of bacterial leaf streak resistant rice varieties or screening of resistance gene resources.

Description

[0001] 【Technical field】 [0002] The invention relates to the field of molecular biology, in particular to the SNP marker location and application of the rice thin stripe resistance gene BLS2. [0003] 【Background technique】 [0004] Bacterial streak disease (referred to as thin stripe disease) is caused by the pathogen Xanthomonas oryze pv. Oryzicola (Xoc), and is an important rice disease. The disease can cause a 32% loss of rice yield in an outbreak year. Cultivation measures and the use of pesticides are effective ways to prevent and control thin stripe disease. However, using resistant resources to breed resistant varieties is the most economical and effective way to control the disease. Genetic analysis showed that there were two different types of resistance resources, such as major genes and quantitative trait loci (Quantitative trait locus, QTL). At present, there are few researches on the application of molecular markers of the major genes of anti-bacterial stripe...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 黄大辉秦钢马增凤岑贞陆刘驰张月雄李振经罗同平
Owner 广西壮族自治区农业科学院水稻研究所
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