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Applications of rice OsMPK1 gene in improvement of disease resistance of rice

A gene, rice technology, applied in the application field of rice OsMPK1 gene in improving rice disease resistance, can solve problems such as the impact of food security situation, the decline of rice yield and quality, etc.

Inactive Publication Date: 2019-01-01
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual production, the impact of various diseases often leads to the decline of rice yield and quality, which has a very serious impact on the food security situation.

Method used

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  • Applications of rice OsMPK1 gene in improvement of disease resistance of rice
  • Applications of rice OsMPK1 gene in improvement of disease resistance of rice
  • Applications of rice OsMPK1 gene in improvement of disease resistance of rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Determining the sequence and structure of the OsMPK1 gene in rice variety Zhonghua 11

[0021] 1. Cloning of the sequence of the OsMPK1 gene in the rice variety Zhonghua 11

[0022] According to Hamel et al. (2006), the LOC_Os06g06090 site in the rice genome sequence database (http: / / rice.plantbiology.msu.edu / ) contains the sequence information of the OsMPK1 gene in the rice variety Nipponbare, which corresponds to The registration number of the cDNA sequence (from Nipponbare) in GenBank (http: / / www.ncbi.nlm.nih.gov) is AK111942, and the length of the cDNA sequence is 1740bp.

[0023]Based on the sequence information published above, the researchers of the present invention designed two pairs of PCR primers MPK1-genome-1F (5'-AGAGCTACAAAACGAGGCCGA-3'), MPK1-genome-1R (5'-GGCCTAGACAAGCAAGAGAATCC-3') and MPK1-genome -2F (5′-TGGCTTTTCTGGTCTGCTTGG-3′), MPK1-genome-2R (5′-GATCAACTCCAAACTGCCACCAC-3′), two DNA fragments containing a partial fragment of the OsMPK1 g...

Embodiment 2

[0030] Example 2: Functional verification of OsMPK1 gene

[0031] The invention adopts the genetic transformation method mediated by Agrobacterium to further verify the function of the gene by overexpressing the OsMPK1 gene in the rice variety Zhonghua 11.

[0032] 1. Construction of genetic transformation vector

[0033] The starting vector used in the present invention is the commonly used vector pU1301 ( Figure 4 , Qiu et al., 2007). It is an Agrobacterium-mediated genetic transformation vector carrying a maize ubiquitin promoter with constitutive and overexpression characteristics.

[0034] Using the T-A cloning vector containing the full-length cDNA sequence of the OsMPK1 gene in Example 1-2 as a template, use the PCR primer 3-MPK1-F (5'- GGTACC ATGGACGCCGGGGCGC-3′) (the underline is the KpnI restriction site) and 3-MPK1-R (5′- GGATCC CTACTGGTAATCAGGGTTGAACGC-3') (the underline is the BamHI restriction site) to amplify the entire coding region of the OsMPK1 gene. ...

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Abstract

The invention belongs to the technical field of plant genetic engineering, specifically relates to applications of a rice OsMPK1 gene in improvement of the disease resistance of rice, and provides isolation cloning and function verification of the DNA fragment of the rice disease-resistance related gene OsMPK1, wherein the nucleotide sequence of the OsMPK1 gene is represented by SEQ ID NO:1, the sequence of the protein encoded by the gene is represented by SEQ ID NO:2, and the encoded product of the protein is a mitogen-activated protein kinase. According to the present invention, the resistance of the transgenic plant over-expressing the OsMPK1 to bacterial leaf streak is significantly enhanced.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering. It specifically relates to the application of rice OsMPK1 gene in improving rice disease resistance. The invention includes the isolation and cloning of the rice OsMPK1 gene and the functional verification and application of regulating rice response to pathogen invasion. OsMPK1 gene promotes disease resistance in rice. The ability of transgenic plants overexpressing OsMPK1 gene to resist rice bacterial stripe disease was significantly enhanced. Background technique [0002] During the growth process, plants are attacked by various pathogens, such as bacteria, fungi, viruses and nematodes. In response to damage, plants have evolved some genes to protect themselves during the long evolutionary process. Discovering and using these genes to improve plant disease resistance is the preferred strategy for disease control under the premise of protecting the environment. [0003] Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N9/12C12N15/82
CPCC12N9/12C12N15/8281C12Y207/11024
Inventor 王石平马海港陈洁
Owner HUAZHONG AGRI UNIV
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