Preparation method of fungus fermentation product and application thereof in prevention and treatment of rice diseases
A technology of fermentation products and fungi, which is applied in the field of preparation of fungal fermentation products, can solve problems such as unresolved industrial production technology and no formal commercial products, and achieve the effects of easy industrial production, good control effect, and simple preparation method
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[0025] The preparation method of fungal fermentation product provided by the invention, its steps are:
[0026] 1) Liquid culture and fermentation product treatment: the fungus Agrocybe sp. YB2005 (preserved in China Center for Type Culture Collection (CCTCC) on March 12, 2012, address: Wuhan University, deposit number CCTCC NO: 2012076) slant strains were first inoculated in a Erlenmeyer flask containing 150mL potato liquid medium Medium-medium activation, the activation conditions are rotation speed 230 r / min, culture temperature 28°C, culture time 7 days, and then carry out large-scale liquid submerged fermentation. The medium formula used is: 200 g of potatoes, 20 g of glucose, and 5 g of peptone per liter of water , natural pH, 0.1Mpa, sterilized at 121°C for 30 minutes, and cultured in a constant temperature shaker at 25-28°C, 180-230 r / min. After 7-12 days of fermentation, the mycelium is separated from the fermentation broth by centrifugation. The fermented liquid ...
Embodiment 1
[0034] Using potato glucose medium (containing 200g of potato, 20g of glucose, 5g of peptone per liter of water, natural pH, 0.1Mpa, sterilized at 121°C for 30min), culture 10 L of activated strain YB2005 in a shaker at 28°C Cultured for 9 d. After the cells were filtered, the fermentation broth was concentrated to 0.5 L, and the fermentation broth was extracted with ethyl acetate. The ethyl acetate part was dehydrated with anhydrous sodium sulfate and then concentrated, then dissolved in methanol and filtered to obtain methanol-soluble crude extract (1.2 g, brown extract).
[0035] Dissolve 1.2 g of the crude methanol extract in the previous step with an appropriate amount of methanol, go through gel (140 g ) column chromatography, and elute with methanol at a flow rate of about 10-15 s / drop, collect 5 mL in each test tube, similar Fractions were combined to obtain Fraction Fr.4 (111 mg) respectively.
[0036] Take 50 mg from Fr.4 (111 mg) in the previous step and dissolve ...
Embodiment 2
[0040] Similar to Example 1, the difference is that the compound structure is identified by NMR (nuclear magnetic resonance) analysis, and the compound structure can be determined according to the compound NMR and MS data.
[0041] from compound 13 C-NMR data show that there are 8 carbons in its structure, and according to the chemical shift value, it is speculated that it is 6 alkyne carbons, 1 oxymethylene and 1 amido carbon (d 153.9s). Using MeOD as the tritiated reagent 1 H-NMR shows that there are two isocarbon protons with the same chemical shift value in its structure, and DMSO is used as the tritiated reagent 1 H-NMR also shows that there are two isocarbon protons with the same chemical shift value in its structure, and three active hydrogens (d 8.42(s,1H), d 7.97(s,1H) and d 5.63(t,6.1,1H) )). The structure of the compound was determined based on the HMBC long-range correlations of H-C(8) with C(7), C(6), C(5) and C(4), which was also supported by the HMBC long-ran...
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