Bacillus pumilus ZR3-3 for controlling and preventing bacterial diseases and application of Bacillus pumilus ZR3-3

A technology of Bacillus pumilus and biocontrol agents, applied in the fields of application, chemicals for biological control, animal repellants, etc., to achieve good application prospects, improve the ecological environment, and reduce the amount of use

Active Publication Date: 2019-05-03
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Bacillus pumilus ZR3-3 (Bacillus pumilus) is a new strain obtained by sampling and isolation by the applicant, which can effectively control Chinese cabbage soft rot, capsicum bacterial wilt, rice bacterial blight, rice bacterial streak and other bacteria Sexually transmitted diseases, no related reports at home and abroad, creative and novel

Method used

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  • Bacillus pumilus ZR3-3 for controlling and preventing bacterial diseases and application of Bacillus pumilus ZR3-3
  • Bacillus pumilus ZR3-3 for controlling and preventing bacterial diseases and application of Bacillus pumilus ZR3-3
  • Bacillus pumilus ZR3-3 for controlling and preventing bacterial diseases and application of Bacillus pumilus ZR3-3

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Inhibitory Effect of Bacillus pumilus ZR3-3 on Pathogenic Bacteria

[0022] A. The pathogenic bacteria tested

[0023] The tested bacteria include cabbage soft rot, capsicum R. solanacearum, rice bacterial blight and rice bacterial streak, provided by the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences. Use LB culture solution (peptone 10.0g, yeast powder 5.0g, NaCl 10g, distilled water 1000mL) at 28°C, 150rpm shaking culture for 2 days, dilute to 10 with sterile water 8 cfu / mL, spare.

[0024] B. Test method:

[0025] The Bacillus pumilus ZR3-3 screened by Jiangsu Academy of Agricultural Sciences was activated on LB medium, then transferred into 50mL LB medium, cultured with shaking at 28°C and 150rpm for 2 days, and diluted with sterile water to 10 8 cfu / mL, spare.

[0026] Take 5 μL of Bacillus pumilus ZR3-3 bacteria liquid, spot it on the center of the LB plate, incubate at 28°C for 48 hours, spray with pathogenic bacteria diluent, incuba...

Embodiment 2

[0031] Metabolic secretions of Bacillus pumilus ZR3-3-production of antimicrobial-related substances

[0032]Test materials: protease detection medium: skimmed milk powder 100g, agar 20g, dilute to 1000mL. Siderophilic detection medium: CAS 60.5mg, 10mL ferric iron solution (1mmol.L -1 FeCl 3 ·6H 2 O), HDTMA 72.9mg, agar 20g, be settled to 1000mL, pH 7.0. Chitinase detection medium: Chitin 15g, MgSO 4 ·7H 2 O 0.5g, FeS0 4 ·7H 2 O0.01g, K 2 HP0 4 0.7g, KH 2 P0 4 0.3g, 20g agar, dilute to 1000mL, pH 7.0-7.2. Cellulase detection medium: peptone 10g, yeast powder 10g, sodium carboxymethylcellulose 10g, NaCl 5g, KH 2 PO 4 1g, 20g agar, dilute to 1000mL, pH 7.0.

[0033] The preparation of the Bacillus pumilus ZR3-3 bacterial solution is the same as in Example 1. Spot 5 μL of Bacillus pumilus ZR3-3 bacterial solution in the center of each test medium plate, and repeat 3 times for each treatment. Incubate at a constant temperature of 28°C for 3-7 days and observe.

...

Embodiment 3

[0041] Obtaining of Bacillus pumilus ZR3-3 water solution

[0042] A. Strain preparation and activation.

[0043] LB culture medium (same as Example 1) is sterilized after adding agar according to conventional methods, and made into a slant. Bacillus pumilus ZR3-3 was inoculated and cultured at 28°C for 2 days.

[0044] B, liquid fermentation.

[0045] First-stage fermentation: Transfer the slant of the above-mentioned cultured test tube into 300mL LB culture solution with an inoculation loop. Shake culture at 28°C and 150rpm for 2 days, and set aside.

[0046] Secondary fermentation: medium formula: bean cake powder 1%, soybean powder 0.5%, yeast extract 0.15%, corn flour 1%, corn steep liquor 0.5%, sucrose 0.5%, NaCl 0.05%, CaCO 3 0.18%. The pH before sterilization is 7.0. Feed 1 ton into a 2 ton fermenter. Sterilize at 121°C for 30 minutes. When cooling to about 30°C, inoculate 300 mL of the above-mentioned primary fermentation seed solution. The liquid fermentatio...

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Abstract

The invention relates to a biologically antibacterial agent. The effective active ingredient of the biologically antibacterial agent is composed of Bacillus pumilus ZR3-3. The biologically antibacterial agent has the characteristics that the biologically antibacterial agent is a water-based agent, and comprises, in percent by weight, 89.5-99.8% of Bacillus pumilus ZR3-3, 0.1-10% of sodium dodecylsulfate and 0.1-0.5% of potassium sorbate. When the biologically antibacterial agent is in use, the water-based agent is poured to roots so as to control soft rot of Chinese cabbages and bacterial wilt of Capsicum annuum, and rice leaf surfaces are uniformly sprayed so that bacterial blight and bacterial leaf streak of rice can be controlled. The biologically antibacterial agent has the advantagesthat through introduction of the Bacillus pumilus ZR3-3 which has human and animal safety and a good control effect on multiple plant bacterial diseases, the biologically antibacterial agent is utilized to control the plant bacterial diseases, the use dosage of chemical agents is reduced, and meanwhile the purposes of improvement of the ecological environment and reduction of the amount of residues of the chemical agents in agricultural products are achieved.

Description

technical field [0001] The invention relates to a biocontrol agent and its application, in particular to a strain of bacillus pumilus ZR3-3 and its application in preventing and treating plant bacterial diseases. Background technique [0002] Bacterial diseases are diseases caused by pathogenic bacteria infection, such as soft rot, bacterial wilt, bacterial wilt, etc. The bacteria that attack plants are rod-shaped bacteria, most of which have 1 to several flagella, which can invade through natural orifices (stomata, lenticels, water holes, etc.) and wounds, and spread by running water, rain, insects, etc. It spends the winter in body, seeds and soil, and it is prone to disease under high temperature and high humidity conditions. Symptoms of bacterial disease are wilting, rot, perforation, etc. In the later stage of the disease, in wet weather, bacterial mucus overflows from the diseased part, with obvious foul smell, which is the characteristic of bacterial disease. At pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N25/02A01P1/00
Inventor 刘邮洲刘永锋周亚秋乔俊卿蒋春华
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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