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Bacillus altitudinis ST15 for antagonizing xanthomonas oryzae and application of bacillus altitudinis ST15

A highlander bacillus and rice technology, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of food safety and ecological environment threats, unsatisfactory control effects, increased application dosage, etc., to improve food and ecological environment safety, strong Tolerance, effect of dose reduction

Active Publication Date: 2020-12-29
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term irrational use of chemical pesticides has led to the gradual increase in the resistance of pathogenic bacteria, the blind increase in the application dose, and the unsatisfactory control effect. The comprehensive control effect on diseases is 50% to 70%. threaten
In addition, due to the high cost and huge investment risk of developing safe and efficient chemical fungicides, few new chemical fungicides have been registered for the control of crop bacterial diseases in recent years

Method used

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  • Bacillus altitudinis ST15 for antagonizing xanthomonas oryzae and application of bacillus altitudinis ST15
  • Bacillus altitudinis ST15 for antagonizing xanthomonas oryzae and application of bacillus altitudinis ST15
  • Bacillus altitudinis ST15 for antagonizing xanthomonas oryzae and application of bacillus altitudinis ST15

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Isolation and identification of Bacillus highlander strain ST15

[0056] (1) Separation of strain resources to be screened: randomly take 50 g of soil from the rhizosphere of the pear orchard, take 10 parts of soil samples in total, mix them evenly, put 10 g of soil into 250 mL of 20 fine glass beads and 100 mL of sterilized deionized water In the Erlenmeyer flask, place on a shaker at 150r / min and shake at 28°C for 1h. After standing for 10 minutes, take 1 mL of the supernatant, dilute it 10 times with sterilized deionized water, draw 100 μL of each concentration gradient dilution, and spread it on the NA solid plate, and culture it at 28°C for 36 hours. Pick a single colony with an inoculation loop and streak and purify it twice on NA solid medium, transfer the purified single colony with different forms to the NA solid plate, culture it upside down at 28°C for 48h, and store it at 4°C for later use.

[0057] (2) Plate antagonism screening of biocontrol strains: Cult...

Embodiment 2

[0067] Growth dynamics of Bacillus uplander ST15 in NB medium

[0068] Take out the ST15 strain preserved in glycerol from the -70°C ultra-low temperature refrigerator, inoculate it on the NA solid plate, and culture it at 28°C for 36 hours; use a sterilized toothpick to pick a single colony of ST15 and inoculate it into a 250mL conical tube containing 50mL NB liquid medium In the flask, cultured at 150r / min and 28°C for 36h as the seed liquid; inoculated the seed liquid into a 250mL Erlenmeyer flask containing 50mL NB liquid medium at a rate of 1% (v / v), 180r / min, Cultivate at 28°C for 84 hours, and measure the absorbance (OD) of the fermentation broth every 6 hours after inoculation. 600 ). Measure the cell density 60 hours after inoculation. The specific method: take samples at the above time points, perform 10-fold gradient dilution with sterilized water, draw 100 μL of each concentration gradient dilution and spread it on a NA solid plate, and incubate at 28°C for 48 hou...

Embodiment 3

[0070] Antibacterial Activity and Stability Analysis of the Supernatant of Bacillus highlander ST15 Fermentation Broth

[0071] Prepare the seed solution of biocontrol bacteria ST15 according to the method of above-mentioned embodiment 2, inoculate in the 250mL Erlenmeyer flask that contains 50mL 50%NB culture medium with the ratio of 2% (v / v), under 28 ℃, 150r / min condition Shake culture, and measure the antibacterial activity of fermentation secondary metabolites 48 hours after inoculation. The specific method: collect samples at the above time points, centrifuge at 10,000g for 15 minutes, filter and sterilize with a 0.22μm bacterial filter, take 1mL of sterile filtrate and pack with In a 1.5mL sterile centrifuge tube, store at -70°C for later use. In order to measure the stability of the ST15 secondary antibacterial substance, the sterile filtrate was treated according to the following methods, specifically: (1) take the subpackaged sterile filtrate, and treat it in a water...

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Abstract

The invention discloses bacillus altitudinis ST15 antagonizing xanthomonas oryzae and application of the bacillus altitudinis ST15. The bacillus altitudinis ST15 is preserved in China General Microbiological Culture Collection Center on June 28th, 2020, and the preservation number of the strain is CGMCC No.20156. The strain has relatively high antagonistic activity to Xanthomonas oryzae pv .oryzaeand Xanthomonas oryzae pv .oryzicola, can promote the growth of rice and improve the drought resistance of rice seedlings, and has relatively strong tolerance to common chemical bactericides for preventing and treating rice bacterial diseases. Bacterial suspension or a biocontrol microbial agent prepared from the strain has a relatively high biocontrol effect on rice bacterial blight and bacterial leaf streak, can replace or reduce the use of chemical pesticides, improves the safety of food and ecological environment, and has relatively high economic and social benefits.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a highlander bacillus ST15 which antagonizes Xanthomonas oryzae and its application. Background technique [0002] Rice xanthomonas (Xanthomonas oryzae), including rice bacterial blight (Xanthomonasoryzae pv. Bacterial blight (BB) and bacterial leaf streak (BLS) are two major bacterial diseases of rice production in my country. The annual occurrence area is 10-15 million mu, and the damage area is 5-8 million mu. It usually results in a loss of 5% to 10% of output, and in severe cases, it can reach more than 50%, or even extinction. In recent years, due to the promotion of light and simplified rice planting models, frequent exchange of germplasm resources, and pathogenic mutations of pathogenic bacteria, the two bacterial diseases not only continue to be prevalent in southern indica rice regions, but also rapidly spread to indica-japonica mixed planting regions in the Yang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P21/00A01P1/00A01P3/00C12R1/07
CPCC12N1/20A01N63/22C12R2001/07C12N1/205A01P15/00A01P3/00A01P21/00
Inventor 刘凤权赵延存苗成琪孙伟波徐会永李朝辉
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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