Application of vitamin B6 content increase in improving resistance of rice to bacterial leaf streak

A vitamin and bacterial technology, applied in the field of plant genetic engineering, can solve problems such as yield and quality decline

Active Publication Date: 2015-12-23
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Rice is an important food crop in the world, but the impact of diseases often leads to the decline of its yield and quality

Method used

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  • Application of vitamin B6 content increase in improving resistance of rice to bacterial leaf streak
  • Application of vitamin B6 content increase in improving resistance of rice to bacterial leaf streak
  • Application of vitamin B6 content increase in improving resistance of rice to bacterial leaf streak

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1 Isolation of OsPDX1 Family Genes and Construction of Plant Expression Vectors

[0066] 1. Isolation of OsPDX1 family genes

[0067] Using the genomic DNA of the susceptible rice variety Zhonghua 11 as a template, OsPDX1.1 was amplified by PCR using primers PDX1.1F / PDX1.1R, PDX1.2F / PDX1.2R, and PDX1.3F / PDX1.3R, respectively. , OsPDX1.2, OsPDX1.3 gene DNA fragments, wherein:

[0068] OsPDX1.1, its nucleotide sequence is shown in SEQIDNo.1, its encoded nucleotide sequence is shown in SEQIDNo.4, and its encoded amino acid sequence is shown in SEQIDNo.7;

[0069] OsPDX1.2, its nucleotide sequence is shown in SEQIDNo.2, its encoded nucleotide sequence is shown in SEQIDNo.5, and its encoded amino acid sequence is shown in SEQIDNo.8;

[0070] OsPDX1.3, its nucleotide sequence is shown in SEQIDNo.3, its coded nucleotide sequence is shown in SEQIDNo.6, its coded amino acid sequence is shown in SEQIDNo.9, 1% agarose gel electrophoresis detection its size (such as fig...

Embodiment 2

[0080] Example 2 Functional verification of OsPDX1 family genes

[0081] The invention uses RNA interference (RNAi) technology and exogenous promoter enhanced expression technology to verify the function of the genes by inhibiting and overexpressing OsPDX1.1, OsPDX1.2 and OsPDX1.3 genes in rice varieties. The main mechanism of the RNAi technology approach: Partial fragments of the target gene are connected in the form of inverted repeats to a vector capable of expressing double-stranded RNA (doublestrandRNA, dsRNA), and the vector is introduced into plants through genetic transformation. The obtained transformed plants express a large number of dsRNAs homologous to partial fragments of the target gene, and these dsRNAs rapidly form short interfering RNA (short interfering RNA, siRNA). These siRNAs are complementary to the transcripts (mRNA) of the target gene, and under the action of special enzymes in the cell, the transcripts of the target gene are degraded, thereby inhibiti...

Embodiment 3

[0127] Example 3 Vitamin B6 improves the resistance of rice to bacterial stripe disease

[0128] The experimental results of the above examples show that the changes in the level of vitamin B6 in the transformed plants are closely related to the changes in the resistance of the plants to bacterial stripe disease. The present invention provides representative and reliable results for illustration. The level of vitamin B6 in CD13-22, a typical positive transformation plant with weakened disease resistance, was significantly reduced compared with Hua 11 in the control material ( Figure 15 ). While the expression of OsPDX1 gene was correspondingly increased in CD13-23 and CD13-25 strains ( Figure 14 ). The results indicated that vitamin B6 positively regulates the resistance of rice to bacterial stripe disease.

[0129] In order to further verify the role of vitamin B6 in the anti-bacterial streak bacterium of rice, the present invention adopts vitamin B6 solution (1mM vitam...

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Abstract

The invention relates to the technical field of plant genetic engineering. Based on the basic discovery that increase of vitamin B6 content can improve the resistance of rice to bacterial leaf streak, the invention utilizes the strong promoter driven transgenic technology to shift the overexpression carrier of vitamin B6 synthetic gene OsPDX1 into rice variety Zhonghua 11, the genetic transformation rice with significantly increased OsPDX1 family gene expression quantity has significantly increased vitamin B6 level, and has significantly enhanced resistance to bacterial leaf streak, while the genetic transformation rice with suppressed OsPDX1 family gene expression has significantly decreased vitamin B6 content, and has significantly reduced resistance to bacterial leaf streak. Therefore, the invention further verifies the important role and application value of vitamin B6 and its synthetic gene OsPDX1 in the resistance of rice to bacterial leaf streak.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to a gene related to improving the resistance of rice to bacterial stripe disease and an application thereof. Background technique [0002] Plants are attacked by various pathogens during their growth. There are many types of plant pathogens, including viruses, bacteria, fungi, and nematodes. Pathogens invade plants and lead to two results: (1) The pathogens successfully reproduce in the host plant, causing related diseases; (2) The host plant produces a disease-resistant response, killing the pathogen or preventing its growth. Using resistance gene resources to improve plant disease resistance is the fundamental way to prevent diseases and protect the environment at the same time. [0003] Plant disease resistance is a complex process regulated by multiple genes. Genes involved in plant disease resistance responses are divided into two categories: (1) disease r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00
Inventor 丁新华储昭辉刘海峰吴涛
Owner SHANDONG AGRICULTURAL UNIVERSITY
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