Methods and systems for co-differentiating cells using optogenetics
By controlling the expression of transcription factors in differentiable cells through optogenetics, utilizing light-activated recombinases and light-inducible promoters in combination with chemical inducers, co-differentiation of multiple cell lineages within a cell population is achieved, solving the problem of insufficient temporal and spatial precision in existing technologies and improving the accuracy and efficiency of differentiation control.
CN120603952APending Publication Date: 2025-09-05プロリフィックマシーンズインク
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Patent Information
- Application Number
- CN202380092373.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2022-12-01
- Filing Date
- 2023-12-01
- Publication Date
- 2025-09-05
AI Technical Summary
Technical Problem
Existing technologies are unable to co-differentiate cells from the same cell population into multiple different cell lineages with high temporal and spatial precision.
Method used
Through optogenetic methods, light-activated recombinases and light-inducible promoters are used to control the expression of transcription factors in differentiable cells, combined with chemical inducers to achieve co-differentiation of cells into multiple cell lineages.
Benefits of technology
It achieves the co-differentiation of cells within a cell population into multiple cell types with high precision in time and space, improving the control accuracy and efficiency of cell differentiation.
✦ Generated by Eureka AI based on patent content.
Abstract
Provided herein are methods and systems for co-differentiating differentiable cells in a differentiable cell population into two or more different cell lineages. In some embodiments, the methods and systems use photoactivatable or chemically activatable recombinase to drive the expression of one or more transcription factors and / or differentiation factors.
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