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Method for extracting original mesenchyma and hematopoiesis trunks/ancestral cell from caesarean birth placenta for treating medulla scathe

A technology of placental tissue and caesarean section, applied in bone diseases, drug combinations, medical formulas, etc., can solve cancer and other problems

Inactive Publication Date: 2008-04-16
上海厚东生物科技有限公司
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Problems solved by technology

Observations show that human mesenchymal stem cells that are routinely expanded for six to eight weeks in vitro are safe, but such stem cells that are cultured in vitro for more than four to five months may become cancerous

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  • Method for extracting original mesenchyma and hematopoiesis trunks/ancestral cell from caesarean birth placenta for treating medulla scathe
  • Method for extracting original mesenchyma and hematopoiesis trunks/ancestral cell from caesarean birth placenta for treating medulla scathe

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Embodiment Construction

[0013] In order to further clarify the outstanding substantive features and remarkable technological progress that the present invention has, the present invention is analyzed in a segmented comparison mode below:

[0014] 1. There are two ways to remove placental tissue from healthy puerpera, one is normal vaginal delivery, the other is caesarean section, the invention proposes that it is better to extract primitive mesenchymal and hematopoietic stem / progenitor cells from caesarean section, which can better ensure the Free from contamination by bacteria and other pathogens.

[0015] In normal vaginal delivery, since the fetus and placenta must escape through a non-sterile vagina, this brings the possibility of microbial contamination, while the fetus and placenta removed by aseptic surgery avoid this possibility. By isolating the placenta and fetus in a sterile environment, our experiments showed that the possibility of microbial contamination of the extracted cells was great...

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Abstract

The present invention is a method for clinic hematopoietic stem cells transplantation by extracting original mesenchymal and hematopoietic stem / progenitor cells from health post-caesarean birth placenta tissue. Each placenta can extract 3.8 + / - 0.7 X 10<9> cells having nucleus, wherein original mesenchymal and hematopoietic stem / progenitor cells are 1.8 + / - 0.2 X10<7>, cell viability is more than 95 percent, microorganism contamination rate is less than 6 percent. The method uses counter monoclonal antibody method to eliminate 90 to 95 percent contaminated mature cell having nucleus single cell in mother blood, reducing immunological rejection reaction (GVHD) after transplanting. The finished product of the method can be used for clinic treating hematopoietic stem cells transplantation due to marrow injury, can be used for applying to placenta tissue supplying person or other patient having various hematopoietic stem cells transplantation indications, and can also combine with various cell growth factors in clinic to develop cell repair and gene treatment. The present invention supplies another cell source for hematopoietic stem cells transplantation, promotes the development of cells transplantation and tissue repairing, also supplies new research direction for early tissue cell differentiation.

Description

technical field [0001] The present invention relates to a method for extracting stem cells from placental tissue in caesarean section and the application of reagents, in particular to a method for extracting primitive mesenchymal and hematopoietic stem / progenitor cells from placental tissue in caesarean section for treating bone marrow injury method. Background technique [0002] At the end of the 20th century, there were two most important developments in the field of life sciences: one was the completion of the DNA sequence determination of the human genome, and the other was a breakthrough in the study of stem cell biology. Various pathogenic genes and physiological genes have been discovered in the human genome. This discipline enables people to understand the mysteries of their own lives and the mechanism of disease occurrence and development, early diagnosis of diseases and timely adoption of effective preventive care and treatment measures. Stem cells are a group of ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/50A61K45/00A61P19/08
Inventor 胡当玉周凝周胜利
Owner 上海厚东生物科技有限公司
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