34results about How to "Safe and efficient preparation" patented technology

The present invention provides a method for subculturing primate embryonic stem cells, and a method for inducing differentiation of the same cell into a vascular endothelial cell and a blood cell. The present invention provides a method comprising culturing primate embryonic stem cells in a medium containing a protein component without using feeder cells and cytokines in a container coated with an extracellular matrix, detaching colonies of the resulting embryonic stem cells in the presence of a cytodetachment agent, and plating the colonies in the similar medium, and a method comprising culturing primate embryonic stem cells in a serum-containing or not containing medium in the presence of cytokine, adhesion-culturing the resulting embryoid body or embroyid body-analogous cellular aggregate in the presence of a cytokine to obtain specific precursor cells, and separating non-adherent cells and adherent cells from the specific precursor cells to obtain blood cells and vascular endothelial precursor cells.

The invention discloses a DC cell and a targeting immune cell population, and a preparation method and application thereof. The preparation method of DC cells includes the steps of: conducting first induced differentiation culture on mononuclear cells in order to obtain immature DC cells; transfecting the immature DC cells by using HSV-2 antigen mRNA, so as to obtain transfected immature DC cells; and conducting second induced differentiation culture on the transfected immature DC cells, so as to obtain mature DC cells, wherein the mononuclear cells are isolated from mononuclear cells of peripheral blood of a sample. The method can prepare DC cells safely and efficiently, has a low cost and high efficiency, and can effectively solve the problem of cytotoxicity. The method can be effectively used for immune cell treatment or stimulation of lymphocytes derived from a same patient to produce a targeting cell population containing a large amount of CTL cells, and then the cell population can be used for virus infection immunotherapy to reach better effect.

The invention provides a sulfide solid electrolyte, a preparation method thereof and an all-solid-state battery. The chemical formula of the solid electrolyte is Li6PS5Cl1-x (BH4)x, and the preparation method comprises the following steps: uniformly mixing Li2S, LiCl, LiBH4 and P2S5 in proportion to obtain a mixture; placing the mixture in a ball milling tank for ball milling; and tabletting the ball-milled sample, and sintering to obtain the sulfide solid electrolyte. According to the sulfide solid electrolyte disclosed by the invention, a BH4 <-> anion group is introduced into the solid electrolyte to replace a part of Cl <-> sites, so that a larger transmission channel is provided; and the conduction of Li < + > is promoted by the unique rotary motion of the BH4 <-> anion group, and after the BH4 <-> anion group is introduced, the ionic conductivity of the electrolyte is greatly improved on the basis of a lithium-argyrodite electrolyte Li6PS5Cl.

The invention relates to a naphthoxy mono-metallocene transition metal compound, a catalyst composition containing the naphthoxy mono-metallocene transition metal compound, and application of the catalyst composition. The naphthoxy mono-metallocene transition metal compound structurally contains naphthoxy, a Cp' ligand and a transition metal, and thenaphthoxy group and the Cp' ligand are respectively connected with the transition metal to form the naphthoxy mono-metallocene transition metal compound. The catalyst composition comprises the naphthoxy mono-metallocene transition metal compound and alkylaluminoxane, and can be used in a polymerization catalyst for olefin polymerization, wherein in the polymerization system, the concentration of the mono-metallocene transition metal compound in the polymerization system is 1*10<-8>-1*10<-3> mol / L. The naphthoxy mono-metallocene transition metal compound is simple to prepare, and when the catalyst composition containing the naphthoxy mono-metallocene transition metal compound is used for olefin polymerization, a polymer with high molecular weight and narrow molecular weight distribution can be obtained.

A connector according to the invention includes: a metal cylinder; a conductive receptacle having an end fitted in an end of the metal cylinder; a pin assembly fitted in the conductive receptacle; pins fixed to the pin assembly, the pins being connected to a cable; a circuit board fixed to the other end of the metal cylinder, the circuit board having through holes electrically connected to the pins; and a sealant disposed between the circuit board and the pin assembly, the sealant infilling gaps between the pins and the through holes of the circuit board.

The invention discloses a rapid preparation method and application of an efficient oil-water separation membrane, and belongs to the field of material surface modification. The preparation method comprises the following steps: (1) carrying out deoiling and decontamination cleaning on the surface of a solid net film; and (2) weighing a hydrophobic polymer material, dissolving the hydrophobic polymer material in a solvent, magnetically stirring at room temperature for 6-8 hours to obtain a uniform modified solution, uniformly coating the pretreated solid net film with the uniform modified solution, and drying. Construction of a solid net film surface micro-nano structure and surface hydrophobic modification are combined into the same system, the overall hydrophobic and oleophylic effects of the material are improved, the material is low in price and easy to obtain, the preparation process is simple and rapid, hazardous reagents such as toxic, high-corrosivity and high-oxidation-reduction reagents are not involved in the preparation process, and the method is suitable for large-scale industrial production; and an oil-water separation net film can realize efficient separation of an oil-water mixture, is high in mechanical property, can well realize self-supporting, and can be freely cut into a required shape during application.

The invention discloses a rotary kiln selenium distillation system which comprises a steel main platform, a slurrying charging system, a sulphating roasting rotary kiln, a selenium absorption system and a production line control system, wherein the slurrying charging system, the sulphating roasting rotary kiln, the selenium absorption system and the production line control system are all arranged on the steel main platform; the slurrying charging system is connected with the left end of the sulphating roasting rotary kiln; the selenium absorption system is connected with the sulphating roasting rotary kiln and comprises a vacuumizing device, a waste heat recovery and smoke discharge system and a selenium absorption device; the sulphating roasting rotary kiln comprises a rotary kiln body and a fuel gas heating system; and a kiln head gas guide pipe is arranged at the upper end of the rotary kiln body and is connected with the selenium absorption device. According to the system disclosed by the invention, large-scale industrial production of selenium products can be realized, and the system has the advantages of high efficiency, high safety and slight pollution.

The invention discloses a DC cell and a targeting immune cell population, and a preparation method and application thereof. The preparation method of DC cells includes the steps of: conducting first induced differentiation culture on mononuclear cells in order to obtain immature DC cells; transfecting the immature DC cells by using CEA antigen mRNA, so as to obtain transfected immature DC cells; and conducting second induced differentiation culture on the transfected immature DC cells, so as to obtain mature DC cells, wherein the mononuclear cells are isolated from mononuclear cells of peripheral blood of a sample. The method can prepare DC cells safely and efficiently, has a low cost and high efficiency, and can effectively solve the problem of cytotoxicity. The method can be effectively used for immune cell treatment or stimulation of lymphocytes derived from a same patient to produce a targeting cell population containing a large amount of CTL cells, and then the cell population can be used for tumor immunotherapy to reach better effect.

The invention provides rotary screen printing white ink and a preparation method thereof. The rotary screen printing white ink comprises the components in parts by mass: 15-30 parts of a polyurethane acrylate oligomer, 3-10 parts of an ultraviolet initiator, 40-55 parts of titanium dioxide, 5-10 parts of dipentaerythritol hexaacrylate, 10-20 parts of hexanediol diacrylate, 0.5-1 part of a dispersing agent, 0.5-2 parts of a leveling agent, 0.2-2 parts of an antifoaming agent and 0.1-0.5 part of a polymerization inhibitor, wherein the polyurethane acrylate oligomer comprises a bifunctional polyurethane acrylate oligomer and a hexafunctional polyurethane acrylate oligomer; and the defoaming agent is selected from one or more of a polyether defoaming agent, an amine defoaming agent and an amide defoaming agent. The rotary screen printing white ink shows a satisfactory printing effect when performing field printing and character printing at the same time, and has excellent and balanced drying speed and adhesion at the same time.

The invention discloses a functional polyquaternary salt and a preparation method thereof. According to the functional polyquaternary salt of the side chain hydroxymethyl propionamide, provided by theinvention, the preparation raw materials comprise 3-(N, N-dialkylamino)propylamine, acrylamide, acrylate, a dialkylation reagent and formaldehyde, the reaction efficiency of each step is high, side reactions are few, the raw materials are easy to obtain, the preparation method is simple, the technological process is safe and efficient, the functional polyquaternary salt laterally connected with hydroxymethyl propionamide has hydrophilicity and an antibacterial function of common quaternary ammonium cation salt, not only can carry out quaternary ammonium cation crosslinking or modification ona natural polymer material, but also can endow the modified material with an antistatic function, a surfactant function and a softener function.

The invention relates to a method of Computed Tomography imaging comprising: a. Performing a single acquisition of image data from at least two contrast agents into a blood vessel network, a first contrast agent among said at least two contrast agents having been in said blood vessel network for a longer time than a second contrast agent among said at least two contrast agents, b. Processing said image data using K-Edge detection and / or iodine delineation to separate data associated with each contrast agents in order to obtain a concentration map of each contrast agent, c. Determining from said image data a first part of the blood vessel network comprising both the first contrast agent and the second contrast agent, and a second part of the blood vessel network comprising only the first contrast agent, d. Calculating a partial blood volume map of the first part of the blood vessel network based on the total amount of second contrast agent and on the concentration map of the second contrast agent, e. Calculating a partial blood volume map of the second part of the blood vessel network based on the total amount of first contrast agent, on the concentration map of the first contrast agent and on the partial blood volume map of the first part of the blood vessel network.

The invention relates to a method of Computed Tomography imaging comprising: a. Performing a single acquisition of image data from at least two contrast agents into a blood vessel network, a first contrast agent among said at least two contrast agents having been in said blood vessel network for a longer time than a second contrast agent among said at least two contrast agents, b. Processing said image data using K-Edge detection and / or iodine delineation to separate data associated with each contrast agents in order to obtain a concentration map of each contrast agent, c. Determining from said image data a first part of the blood vessel network comprising both the first contrast agent and the second contrast agent, and a second part of the blood vessel network comprising only the first contrast agent, d. Calculating a partial blood volume map of the first part of the blood vessel network based on the total amount of second contrast agent and on the concentration map of the second contrast agent, e. Calculating a partial blood volume map of the second part of the blood vessel network based on the total amount of first contrast agent, on the concentration map of the first contrast agent and on the partial blood volume map of the first part of the blood vessel network.

A process for the production of N-aryl-2,2,2-trifluoro- acetimidoyl chloride represented by the general formula (2) by reacting a 2,2,2-trifluoro-N-arylacetamide represented by the general formula (1) with both at least one member selected from the group consisting of phosphorus oxychloride and diphenyl- phosphoric acid chloride and a tertiary amine in an organic solvent; and a process for the production of 1-aryl-5 -(trifluoromethyl)-1H-tetrazoles represented by the general formula (4) by reacting an N-aryl-2,2,2-trifluoroacetimidoyl chloride represented by the general formula (2) with an azide in an aromatic hydrocarbon solvent in the presence of an amine salt.

The invention belongs to the technical field of metal additive manufacturing, and particularly relates to nickel-based alloy powder and a preparation method and application thereof. The nickel-based alloy powder is used as a coating layer to coat composite ceramic reinforced particles. The nickel-based alloy powder with the structure can effectively solve the thermal cracking tendency of the original nickel-based alloy powder in the printing process, and solves the problems that the added ceramic particle heterogeneous nucleating agent cannot be uniformly and stably distributed on the nickel-based alloy powder and cannot meet the large-scale production requirement.

The invention discloses a DC cell based on HBV1 antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of the DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by HBV1 antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, the DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.

The invention provides an environment-friendly ultraviolet-cured oil with a fluffy tactile impression and a production method of the environment-friendly ultraviolet-cured oil with the fluffy tactileimpression. The environment-friendly ultraviolet-cured oil with the fluffy tactile impression comprises, in parts by weight, 60-90 parts by weight of a modified bifunctional polyurethane acrylate oligomer, 3-10 parts by weight of an ultraviolet initiator, 5-10 parts by weight of an amine co-initiator, 2-5 parts by weight of extinction powder, 5-15 parts by weight of tactile powder, 0.5-1 part by weight of a dispersion agent, 0.5-1 part by weight of a flatting agent and 0.1-1 part by weight of a defoaming agent; and the relative molecular mass of the modified bifunctional polyurethane acrylateoligomer is 2700-3000, and the viscosity at 25 DEG C is 80-150 cps. The tactile oil not only is environmentally friendly, but also has the advantages that a curing source is single, the stability is high, the anti-wear property is excellent, and the fluffy tactile impression is strong.

The invention relates to a novel mono-metallocene transition metal compound, a catalyst composition containing the same and application. The structure of the novel mono-metallocene transition metal compound contains a Cp' ligand, and contains a quinoline group. The catalyst composition comprises the mono-metallocene transition metal compound and alkylaluminoxane, and can be used in a polymerization catalyst for olefin polymerization, and in the polymerization system, the concentration of the mono-metallocene transition metal compound in the polymerization system is 1*10<-8> mol / L to 1*10<-3> mol / L. The novel mono-metallocene transition metal compound is simple to prepare, and a polymer with high molecular weight and narrow molecular weight distribution can be obtained when the catalyst composition containing the mono-metallocene transition metal compound is used for olefin polymerization.

A method for preparing synthetic gas from volatiles in low rank coal is characterized in that the low rank coal is processed by a gasification reduction process to obtain an oil and gas mixture, the oil and gas mixture is processed by a purifying process to obtain a mixed gas containing CO, H2 and hydrocarbons, the mixed gas is processed by a partial hydrocarbon reforming conversion process to obtain the synthetic gas containing CO and H2, and the gasification reduction process is a chemical reaction process for heating the dried low rank coal under an oxygen-free or micro-aerobic condition. The method and the system fully and effectively use the volatiles in low rank coal to prepare the synthetic gas, so the calorific value of the synthetic gas is high; and devices of the system are simple, and are easy to operate, and most of the devices are existing devices , so the system has the advantages of low running cost, simple process, high safety, ensuring of safe and efficient preparationof the synthetic gas, and realization of reasonable and full utilization of products generated in the whole processing process of the low rank coal through the arrangement design of the system devices.

The invention provides a method for preparing synthesis gas by graded utilization of low-rank coal. According to the method, low-rank coal is sequentially treated through a drying process and a gasification reduction process to obtain upgraded coal and an oil-gas mixture, the oil-gas mixture is treated through a purification process to obtain mixed gas containing CO, H2 and hydrocarbons, and the mixed gas is treated through a separation process to obtain first synthesis gas containing H2 and CO and gas containing hydrocarbons; the hydrocarbon-containing gas is subjected to a reforming conversion process to produce second synthesis gas comprising CO and H2; the upgraded coal reacts with H2O and O2 to prepare third synthesis gas containing CO and H2; and the first synthesis gas, the second synthesis gas and the third synthesis gas are mixed to obtain the synthesis gas. According to the method and the system disclosed by the invention, volatile components and fixed carbon in low-rank coalare retained in the forms of CO and H2 to the greatest extent in multiple ways, the yield of the synthesis gas is high, and the low-rank coal is fully and effectively utilized.

The invention discloses a DC cell based on SURVIVIN antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by SURVIVIN antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.

The invention relates to an environment-friendly botanic pesticide. The environment-friendly botanic pesticide is prepared from sodium alginate, gellan gum, borneol, ethylicin, semiliquidambar cathayensis radix, linamarin, a surfactant, rhizoma dioscoreae bulbiferae, herb of achnatherum inebrians, salt, ethanol and water. According to the environment-friendly botanic pesticide provided by the invention, the environment-friendly pesticide is reasonable in preparation and high in drug effect and is free of residual and toxic hazards, the raw materials are wide in source and low in cost, and meanwhile, the drug effect is lasting, so that the environment-friendly pesticide is safe and efficient; the preparation process is safe, is free of environmental pollution and is easy to popularize and apply.

The invention discloses a DC cell based on HPVE6 antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by HPVE6 antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, the DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.

The invention discloses a waterproof adhesive based on polycaprolactone and a preparation method thereof.The waterproof adhesive based on polycaprolactone is prepared from, by weight, 30-40 parts of polycaprolactone, 30-40 parts of paraffin and 45-60 parts of EVA. According to the waterproof adhesive based on polycaprolactone, the flame-retardant material mixture is arranged in the preparation process, the preparation method is matched, and the waterproof adhesive based on polycaprolactone is prepared; compared with the prior art, the organic solvent provided by the invention can be used for protecting internal materials such as polycaprolactone, paraffin and epoxy resin when a polyurethane polymer is prepared, so that the natural problem in a high-temperature environment is avoided; the problem that the risk of ignition or explosion occurs in the preparation process is solved, so that the safety of the waterproof adhesive in the preparation process is greatly improved.

The invention discloses a method for preparing para-aramid fibers by spraying method. By using p-phenylenediamine and terephthaloyl chloride as monomers and nitrogen methyl pyrrolidone as solvents, the monomers are dissolved in an inert gas environment. The dispersed phase is formed through spray gun atomization, and the mist clusters collide to quickly undergo polycondensation reaction, and the product is directly sprayed on the material panel, and after high-temperature drying, an aramid coating is formed in one step. During the reaction process, the atomization state is used to increase the contact specific surface area of ​​the reactants, so as to realize the rapid removal of reaction heat and improve the process safety. The invention realizes rapid removal of reaction heat in the aramid fiber polymerization process, the production process is safe and controllable, and the thermal insulation coating material can be manufactured more efficiently.

The invention discloses continuous self-cleaning glass substrate growth equipment which comprises a feeding and receiving transition cabin mechanism, an air cylinder pushing mechanism, a quartz tube,a first heating furnace, a second heating furnace and an equipment frame. The quartz tube is cylindrical and is fixedly arranged on the equipment frame; the feeding and receiving transition cabin mechanism comprises a discharging cabin, a receiving cabin and a quartz tube; the bottoms of the two ends of the quartz tube are fixedly connected with the equipment frame through supporting frames. The wall portion of one end of the quartz tube is communicated with a discharging bin through a flange, a first gate valve is arranged at the joint of the flange, a first air cylinder is arranged at the end of the discharging bin, and the first air cylinder is connected with a first push rod extending into a cavity of the discharging bin. The equipment is an innovative invention developed on the basisof a carbon dioxide etching method, has the characteristics of simplicity, mildness, low cost and releasability through the carbon dioxide etching method, and is very suitable for large-area and low-cost batch preparation of the ultra-clean graphene film.

The invention discloses a DC cell based on HM1.24 antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by HM1.24 antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.

The invention provides a method for preparing Pd nanoparticles, which belongs to the technical field of organic synthesis. It includes the following steps: using cheap aniline as a starting material, preparing an ethanol solution of aniline and tetrafluoroboric acid, and adding tert-butyl nitrite dropwise to the solution under ice bath conditions to obtain phenyldiazo tetrafluoroborate. ; Mix phenyldiazonium tetrafluoroborate and Pd acetate into a uniform solution, stir and react at room temperature, then add sodium borohydride methanol solution dropwise under ice bath conditions, and obtain Pd nanoparticles after the reaction. The invention uses aniline as the starting substrate, is easy to operate, safe and low in cost, and the obtained Pd nanoparticles have the characteristics of high reactivity and difficulty in deactivation.

The invention discloses a DC cell based on HPVE7 antigen, a targeting immune cell population, a preparation method and applications thereof. The preparation method of DC cell comprises the following steps: subjecting mononuclear cells to first induced differentiation culture to obtain immature DC cells; transfecting the immature DC cells by HPVE7 antigen mRNA to obtain transfected immature DC cells; subjecting the transfected immature DC cell to second induced differentiation culture to obtain mature DC cells; wherein the mononuclear cells are separated from peripheral blood mononuclear cells of a sample. Through the provided preparation method, DC cells can be obtained efficiently and safely. Moreover, the provided preparation method has the advantages of low cost and high efficiency, and can effectively solve the problem of cell toxicity. The DC cells can be applied to cell immunotherapy or used to trigger the lymph cells from the same patient to generate targeting immune cell population rich in CTL cells so as to achieve a better effect in tumor immunotherapy.