DC cell based on HPVE7 antigen, targeting immune cell population, preparation method and applications thereof
A technology of cells and antigens, applied in the field of targeted immune cell populations and their preparation, and DC cells, can solve the problems of immune cell preparation methods that need to be improved, and achieve the effect of solving the problem of cytotoxicity, high preparation efficiency, and low cost
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Embodiment 1
[0065] 1. Materials and equipment
[0066] 1. Laboratory level: GLP
[0067] 2. Instruments and equipment:
[0068] Negative 80-degree ultra-low temperature refrigerator (Zhongke Meiling, DW-HL388); ordinary refrigerator (Haier, double door, -20°C and 4°C); mold incubator (Shanghai Boxun, MJX-160B-Z); constant temperature water bath ( SSW-420-2S); CO 2 Incubator (HF240); low-speed centrifuge (Shanghai Anting, TDL-40C); inverted biological microscope (Kexin, model: BLD-1); biological safety cabinet (Suzhou purification, double); Rand) 1-10ml; 1 set of micropipettes (Dalong), 4 pieces; programmed cooling box (Thermo); liquid nitrogen tank (Jinfeng, YDS-120-216); PCR instrument; gel electrophoresis imaging system ; Electrophoresis apparatus; Thermostat;
[0069] 3. Cell culture consumables:
[0070] Cell culture bag (Takara company); 75cm 2 Culture bottle, 175cm 2 Culture bottle, 1.8ml cryopreservation tube, 1.5ml EP tube, 50ml centrifuge tube, and 15ml centrifuge tube are...
Embodiment 2
[0132] Embodiment 2: clinical treatment
[0133] The CTL cells prepared in Example 1 were reinfused into the patient, and the reinfusion effect was observed, as follows:
[0134] 20 HPVE7 positive cervical cancer patients were divided into 2 groups (10 in group A and 10 in group B), wherein, for group A, a sufficient amount of CTL cells were obtained on day 14 and day 15 according to the method in Example 1 After that, reinfuse the patient, and the amount of cells reinfused each time is 1~2*10 9 ; Group B did not reinfuse cells (as a control). One blood collection and two consecutive reinfusions constituted a course of treatment. After 3 courses of treatment, observation was carried out.
[0135] The results showed that there was a significant difference between group A and group B. After reinfusion, all patients in group A felt less tired, had increased appetite, lessened pain, and gained weight. Among them, 6 patients had decreased tumor indicators; while The symptoms of...
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