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30results about How to "Change the microenvironment" patented technology

Nano magnetic microsphere supported TEMPO catalyst and synthetic method and application thereof

The invention discloses a nano magnetic microsphere supported TEMPO catalyst and a synthetic method and application thereof. The structure of the catalyst is shown as a formula (I-1) or formula (I-2); the catalyst (I-1) is composed of a nano magnetic microsphere (II), a linking group A and a catalyst TEMPO; one end of the linking group A is connected with C* of the nano magnetic microsphere II, and the other end is connected with the catalyst TEMPO; the linking group A is selected from the groups shown as A (1), (2), (3) and (4); the cationic part (III) of the catalyst (I-2) is obtained by connecting the linking group (IV) and the C* of the nano magnetic microsphere (II); the nano magnetic microsphere (II) is formed by wrapping a polystyrene-poly p-chloromethyl styrene copolymer coating layer outside the nano Fe3O4 magnetic core. The catalyst involved in the invention can be used in the oxidation of alcohols. The catalyst provided by the invention does not agglomerate or coagulate in the preparation and reaction process, has significantly increased catalytic activity and phase transfer catalytic function.
Owner:ZHEJIANG UNIV OF TECH

Cultivation method for rice direct dibble seeding in wedged ditches

The invention discloses a cultivation method for rice direct dibble seeding in wedged ditches, which belongs to the field of rice cultivation. In order to solve the problem that yield potential of high-yield high-quality varieties is difficult to fulfill by existing cultivation methods, the cultivation method for rice direct dibble seeding in wedged ditches includes the steps: firstly, tilling soil, namely performing no turning or irrigation after preceding harvesting, and digging a field to obtain a plurality of ditches, wherein row spacing between each two adjacent ditches is 18-22cm, each ditch in a triangular shape is wide end up, each ditch is 5-20cm deep and 3-6cm wide, two sides of each ditch are slopes relative to the horizontal plane, and an included angle between each slope and the horizontal plane is 40 DEG-70 DEG; secondly, post-treating rice seeds; thirdly, performing base fertilizer application, direct dibble seeding, and irrigation; and fourthly, performing field management. The traditional ploughing is replaced with wedging, so that farming intensity is reduced, interference to soil is lowered, energy sources are saved, labor is reduced, seeds for use are few, soilwater retention capacity is high, soil system stable is high, fertilizer utilization rate is high, root system growth environment is improved, and cost reducing, yield increase and efficiency enhancement are achieved.
Owner:HUNAN AGRICULTURAL UNIV

Tube seedling slow growth conservation method for anoectochilus roxburghii germplasm

The invention provides a tube seedling slow growth conservation method for anoectochilus roxburghii germplasm. The method comprises the following steps: S1, material drawing and sterilization of explants; S2, inoculation and induction; S3, subculture multiplication; S4, low-temperature stress treatment of tube seedlings; S5, slow growth conservation of the tube seedlings; S6, rejuvenation of the tube seedlings; S7, rooting of the tube seedlings; S8, transplantation. The method effectively slows the growth speed of the tube seedlings by changing microenvironment conditions for the growth of germplasm materials, prolongs the subculture cycle, ensures that the germplasm preservation time reaches 3 to 4 years, is stable in inheritable character, high in germplasm survival rate and obvious in rejuvenation effect, and has important significance on further protection of the anoectochilus roxburghii resource and research, development and utilization of anoectochilus roxburghii.
Owner:谭兴江

High-throughput microfluidic chip and cell analysis device and method

The invention discloses a high-throughput microfluidic chip and a cell analysis device and method. The high-throughput microfluidic chip comprises a cell observation pool, a stimulant channel, a cell introduction channel, a buffer solution channel and a waste liquid channel, wherein the stimulant channel is connected with the opening of the cell observation pool, the cell introduction channel is connected with the side face of the cell observation pool, the connecting site is close to the opening, the buffer solution channel is connected with the bottom of the cell observation pool, and the waste liquid channel is connected with the opening of the cell observation pool. The cell analysis device comprises the high-throughput microfluidic chip, a signal collection device and electric proportional switching valves, and the channel inlet end of the high-throughput microfluidic chip is connected with independent electric proportional switching valves; the signal collection device is arranged at the cell observation pool and is used for collecting cell signals or imaging cells. The analysis method comprises the following steps: capturing cells, stimulating cells and releasing cells. The chip, the device and the method provided by the invention can be used for achieving high-throughput cell stimulation and analysis in multiple modes.
Owner:HUAZHONG UNIV OF SCI & TECH

Composition for treating acne as well as preparation method and application thereof

The invention relates to a composition for treating acne as well as a preparation method and application thereof. The composition is prepared from the following raw materials in parts by weight: 400 to 1,200 parts of Vaseline, 8 to 80 parts of benzoic acid, 4 to 40 parts of boric acid, 6 to 60 parts of salicylic acid, 40 to 120 parts of ichthammol, 1 to 10 parts of vitamin E, 2 to 15 parts of borneol, 10 to 40 parts of radix sophorae flavescentis, 5 to 25 parts of radix scutellariae, 5 to 25 parts of cortex phellodendri chinensis, 5 to 25 parts of rhizoma coptidis, 10 to 40 parts of radix et rhizoma rhei and 20 to 120 parts of plant essential oil. The composition has the advantages of being short in medication cycle, obvious in curative effects and convenient to use and not influencing daily makeup in a use process. The composition for treating the acne, which is provided by the invention, can be used for quickly permeating a skin, is used for promoting the skin to absorb a medicine, changing a microenvironment, clearing away heat and toxic materials, removing the symptoms of redness, swelling and pain, balancing grease secretion, enabling skin to be fresh, cool, bright and clean, and quickening the skin to heal, is insecticidal and anti-inflammatory, and is applicable to the treatment of the acne which is to various extents and the prevention of the formation of the acne and the mark of the acne.
Owner:杨德芬

Composite nano-drug constructed by nanoparticles coupled with pharmaceutical ingredients as well as preparation method and application of composite nano-drug

The invention discloses a composite nano-drug constructed by nanoparticles coupled with pharmaceutical ingredients as well as a preparation method and an application of the composite nano-drug, and belongs to the technical field of composite drugs. The method for preparing the nano-drug comprises the following operations: modifying amino on the surfaces of nanoparticles which contain hydroxy in the surfaces, and activating biological or small-molecular drugs containing hydroxy; and then conducting a conjugate coupling reaction on the nanoparticles and the drugs, so that a plurality of chemicalbonds are constructed, combining the plurality of chemical bonds so as to prepare the nano-drug. Based upon researches, it is indicated that an anti-cancer effect can be greatly improved on the basisof a synergistic effect between active ingredients of natural drugs (such as taxol, ginsenoside and other traditional Chinese medicines) and a nano-material, and meanwhile, key and breakthrough points can be also provided for researching and producing efficient anti-cancer nano-drugs which are low or free from toxic and side effects and have targeting and autonomous targeting performances.
Owner:郑州天兆医疗科技有限公司

In vitro development of tissues and organs

A method for producing a differentiated tissue is provided, which method comprises isolating at least one population of homogeneous multipotent cells, and culturing the cells in the presence of at least one differentiation factor, wherein the cells are supported by a solid phase scaffold. Differentiated tissues produced by this method, and there medical uses are also described. A method of screening for a process of producing a given differentiated tissue, and a method of optimizing a process of producing a given differentiated tissue are also described.
Owner:TRANSFORM PHARMACEUTICALS INC

Cultivation method for wedge plowing and transplanting rice

The invention discloses a cultivation method for wedge plowing and transplanting rice, belonging to the field of rice cultivation. To solve the problem of the low yield of high-yield high-quality varieties in the exiting cultivation mode, the cultivation method of the invention comprises the following steps: (1) plowing soil; not carrying out plowing and watering after harvesting former crop in rotation; and digging a plurality of ditches on a surface of a farmland, wherein the row spacing between two adjacent ditches is 20cm, the ditch is narrow at the bottom and wide at the top and has an inverted triangular shape, the depth of the ditch is 5-10cm, the width of the ditch is 3-6cm, the two side faces of the ditch are all oblique planes relative to a horizontal plane, and the included angle alpha is 40-70 degrees between the oblique plane and the horizontal plane; (2) applying base fertilizer, transplanting seedlings, and watering; and (3) subsequently managing the field. The cultivation method of the invention reduces the farming intensity by using wedge plowing instead of traditional ploughing, applies fertilizer to the bottom of the wedge-type ditch in a centralized way, reduces the disturbance to the soil, is energy-saving and labor-saving, has large water storage capacity of the soil and high soil system stability, increases the utilization ratio of fertilizer, improves the growth environment of root system, and realizes the effects of saving cost and increasing yield and efficiency.
Owner:HUNAN AGRICULTURAL UNIV

Near ultraviolet excited charge compensation type red-light phosphate light emitting material and preparation method thereof

The invention provides a near ultraviolet excited charge compensation type red-light phosphate light emitting material and a preparation method thereof. The near ultraviolet excited charge compensation type red-light phosphate light emitting material chemically consists of Sr2-2xP2O7:xEu<3+>, xM<+>, wherein Eu<3+> is a light emitting center; M<+> is a charge compensation agent; M=Li, Na or K; and x=0.02-0.16. Na<+> is a charge compensation agent with the optimal property and can improve the emission intensity and the chromaticity of red light to the maximum extent. The preparation method comprises the following steps: by taking a high temperature solid state method, weighing raw materials SrCO3, Li2CO3, Na2CO3, K2CO3, (NH4)2HPO4 and Eu2O3 according to a chemical weighing ratio of target products, uniformly grinding, calcining in the air atmosphere, and performing furnace cooling to the room temperature, thereby obtaining a target product. The preparation method has the characteristics of being simple to operate, sufficient in reaction, applicable to production and the like, and the prepared light emitting material is high in light emission efficiency, high in brightness, good in chromaticity and good in stability.
Owner:SHAANXI UNIV OF SCI & TECH

Cell affinity agent capable of improving duck flavivirus in-vitro infection efficiency and preparation method of cell affinity agent

The invention discloses a cell affinity agent capable of improving duck flavivirus in-vitro infection efficiency and a preparation method of the cell affinity agent and belongs to the technical field of agents for microbial virus culture. The cell affinity agent is prepared from the following components in percentage by mass: 0.5-5% of organic acid, 0-1% of phosphoric acid, 0-3.5% of inorganic salt and the balance of deionized water. By the cell affinity agent, the microenvironment between the duck flavivirus and the cell can be improved, and the affinity between the duck flavivirus and the cell is increased so that the infection efficiency of the duck flavivirus to the cell is effectively increased.
Owner:GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST +1

Method for treating escherichia coli in tail water of sewage plant

The invention relates to a method for treating escherichia coli in tail water of a sewage plant. The method comprises the following steps of: introducing the tail water of the sewage plant into a bioretention pond filled with anthracite serving as a filler; inoculating arbuscular mycorrhizal fungi into aquatic plants in a seedling stage to construct an arbuscular mycorrhizal fungi-aquatic plant symbiotic system; and planting the arbuscular mycorrhizal fungi-aquatic plant symbiotic system in the bioretention pond. According to the method of the invention, the escherichia coli in the sewage is intercepted and eliminated in a manner of combining physical action and biological action.
Owner:江苏金陵环境股份有限公司 +1

External traditional Chinese medicine composition and preparation for treating nodular type acnes and preparation method thereof

The invention relates to an external traditional Chinese medicine composition and preparation for treating nodular type acnes and a preparation method thereof. The composition comprises the following raw materials in parts by weight: 5-10 of nagkassar seeds, 10-25 of bark of magnolia, 10-20 of Chinese angelica, 20-30 of cajeput leaves, 10-20 of alfalfa, and 5-10 of motherwort. The traditional Chinese medicine extract provided by the invention has the advantages of eliminating causes of disease causing arcane inflammation by controlling bacteria breeding on the one hand and improving skin quality by controlling sebum secretion and conditioning the water-oil balance of skins on the other hand; and more importantly, the traditional Chinese medicine extract can improve skin microcirculation, change skin microenvironment and fundamentally eliminate nodular type acne cyst, acne marks and scars.
Owner:BEIJING DONGFANG MIAOSEN BIOTECH CO LTD

A kind of immobilized horseradish peroxidase carrier and its preparation and application method

The invention belongs to the field of biotechnology, and specifically relates to an immobilized horseradish peroxidase carrier and its preparation and application method, wherein the immobilized horseradish peroxidase carrier is nano-carbon spheres prepared by hydrothermal carbonization of glucose , and then it is obtained by amination modification and glutaraldehyde activation; the immobilized horseradish peroxidase carrier of the present invention is mixed and shaken with the horseradish peroxidase solution, centrifuged, washed and freeze-dried to obtain the immobilized horseradish peroxidase root peroxidase. The invention provides a green, economical and efficient method for preparing immobilized horseradish peroxidase, the immobilized horseradish peroxidase obtained by the method is low in cost, simple in operation, stable in physical and chemical properties, especially in extreme environments High catalytic activity can still be maintained under these conditions.
Owner:ANHUI UNIVERSITY

A high-throughput microfluidic chip, cell analysis device and method

The invention discloses a high-throughput microfluidic chip and a cell analysis device and method. The high-throughput microfluidic chip comprises a cell observation pool, a stimulant channel, a cell introduction channel, a buffer solution channel and a waste liquid channel, wherein the stimulant channel is connected with the opening of the cell observation pool, the cell introduction channel is connected with the side face of the cell observation pool, the connecting site is close to the opening, the buffer solution channel is connected with the bottom of the cell observation pool, and the waste liquid channel is connected with the opening of the cell observation pool. The cell analysis device comprises the high-throughput microfluidic chip, a signal collection device and electric proportional switching valves, and the channel inlet end of the high-throughput microfluidic chip is connected with independent electric proportional switching valves; the signal collection device is arranged at the cell observation pool and is used for collecting cell signals or imaging cells. The analysis method comprises the following steps: capturing cells, stimulating cells and releasing cells. The chip, the device and the method provided by the invention can be used for achieving high-throughput cell stimulation and analysis in multiple modes.
Owner:HUAZHONG UNIV OF SCI & TECH

Nano magnetic microsphere supported tempo catalyst and its synthesis method and application

The invention discloses a nano magnetic microsphere supported TEMPO catalyst and a synthetic method and application thereof. The structure of the catalyst is shown as a formula (I-1) or formula (I-2); the catalyst (I-1) is composed of a nano magnetic microsphere (II), a linking group A and a catalyst TEMPO; one end of the linking group A is connected with C* of the nano magnetic microsphere II, and the other end is connected with the catalyst TEMPO; the linking group A is selected from the groups shown as A (1), (2), (3) and (4); the cationic part (III) of the catalyst (I-2) is obtained by connecting the linking group (IV) and the C* of the nano magnetic microsphere (II); the nano magnetic microsphere (II) is formed by wrapping a polystyrene-poly p-chloromethyl styrene copolymer coating layer outside the nano Fe3O4 magnetic core. The catalyst involved in the invention can be used in the oxidation of alcohols. The catalyst provided by the invention does not agglomerate or coagulate in the preparation and reaction process, has significantly increased catalytic activity and phase transfer catalytic function.
Owner:ZHEJIANG UNIV OF TECH

A method for strengthening and fixing endogenous phosphorus in eutrophic water sediment

The invention provides a method for reinforcing and fixing endogenous phosphorus on eutrophic water body bottom mud. The method comprises the following steps that 1, water purification plant sludge is filtered with an iron screen, dried, crushed and then sieved by a 100-mesh sieve to obtain treated water purification plant sludge; 2, anhydrous calcium chloride powder sieved by the 100-mesh sieve is doped into the treated water purification plant sludge, and the materials are mixed to be uniform by a stirrer to obtain a mixed material; 3, the mixed material is fired to obtain an endogenous phosphorus curing material; 4, the endogenous phosphorus curing material is tiled on a surface layer of the remediated eutrophic water body bottom mud; 5, corbicula fluminea is introduced; 6, the endogenous phosphorus curing material is uniformly laid on the surface layer of the remediated eutrophic water body bottom mud again, and then the step of reinforcing and fixing the endogenous phosphorus on the eutrophic water body bottom mud is completed. According to the method, the bottom mud interior microenvironment can be changed, a space and a state in which anaerobism and aerobism alternatively exist are formed, and mutual transformation between the endogenous phosphorus forms is reinforced.
Owner:SUZHOU UNIV OF SCI & TECH

A kind of discene-perylene diimide derivative and its synthesis method and its sensing application to gas-phase volatile aromatic hydrocarbons

The invention discloses a ptycene-perylene diimide derivative, a synthesis method thereof and sensing application to gas-phase volatile aromatic hydrocarbon. The ptycene-perylene diimide derivative has a special non-planar structure, so that a prepared sensing film has large specific surface area and porosity, and is rich in molecular channels, and good diffusion of molecules of an object to be detected in the film can be ensured; and meanwhile, by utilizing a capillary condensation phenomenon and the sensitive effect of fluorescent molecules on a surrounding environment in a high-excitation state, high sensitivity and high selectivity response to BTEX gas are realized, the response time and the recovery time are short, and a new thought is provided for gas-phase sensing of volatile organic compounds lacking photoelectric activity and preparation of a fluorescent sensing film with excellent performance. The preparation method is simple and convenient to operate and mild in reaction condition, the photochemistry stability and thermodynamic stability of a prepared fluorescent compound are good, and the obtained fluorescent sensing film is high in sensitivity, good in selectivity andlong in service life, and is an excellent fluorescent sensing film.
Owner:SHAANXI NORMAL UNIV

A kind of cell affinity agent and preparation method thereof for improving in vitro infection efficiency of duck flavin virus

The invention discloses a cell affinity agent capable of improving duck flavivirus in-vitro infection efficiency and a preparation method of the cell affinity agent and belongs to the technical field of agents for microbial virus culture. The cell affinity agent is prepared from the following components in percentage by mass: 0.5-5% of organic acid, 0-1% of phosphoric acid, 0-3.5% of inorganic salt and the balance of deionized water. By the cell affinity agent, the microenvironment between the duck flavivirus and the cell can be improved, and the affinity between the duck flavivirus and the cell is increased so that the infection efficiency of the duck flavivirus to the cell is effectively increased.
Owner:GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST +1

A biodegradable phosphorus-magnesium whisker medical material capable of inhibiting the growth of cancer cells

The invention aims at providing a novel degradable phosphorus-magnesium crystal whisker medical material with a cancer cell growth restraining effect. The phosphorus-magnesium crystal whisker medical material is characterized by being a magnesium salt compound with main component elements of phosphorus, magnesium and oxygen; the phosphorus, magnesium and oxygen content is larger than 60wt%, morphology of the material is columnar-shaped or flaky-shaped fluffy powder, the diameter is smaller than 50mu m, the length is larger than 100mu m, and the ratio of length to diameter is larger than 10 times. The degradable phosphorus-magnesium crystal whisker medical material is in a solid fluffy fiber shape and can change a pH value of a cancer cell growth microenvironment to restrain cancer cell growth in degrading. When crystal whisker fiber is prepared into liquid or is mixed with other solution to be prepared into liquid, an effect of restraining cancer cell growth is also achieved. By means of the effect of the crystal whisker material, the crystal whisker material can serve as a medical material for restraining cancer cells.
Owner:INST OF METAL RESEARCH - CHINESE ACAD OF SCI

Planting method for improving growth and yield of corn

The invention belongs to the technical field of agricultural planting, and particularly relates to a planting method for improving the growth and yield of corn. The planting method for improving the growth and yield of the corn is characterized in that straw full-amount deep tillage treatment operation is performed after wheat is harvested; the corn is sown after the operation is completed, and the full-amount deep tillage depth of straw is not smaller than 30 cm. By adopting the planting method for improving the growth and yield of the corn, the soil structure and permeability can be improved, corn root growth and water and fertilizer absorption and utilization are facilitated, high yield and high efficiency are achieved, and technical support is provided for large-area efficient green production of summer corn.
Owner:济宁市农业科学研究院

Composite traditional Chinese medicine preparation as well as preparation method and application thereof

The invention discloses a compound traditional Chinese medicine preparation as well as a preparation method and application thereof. The compound traditional Chinese medicine preparation comprises thefollowing raw materials of chitosan oligosaccharide, grape seed extract, fructus corni, balsam pear, radix rehmanniae preparata, radix notoginseng, fructus lycii, radix salviae miltiorrhizae, radix angelicae sinensis, rhizoma polygonati, radix ophiopogonis, radix asparagi, rhizoma coptidis, radix glycyrrhizae preparata, folium nelumbinis, cortex lycii radicis, fructus crataegi, mulberries, cortexeucommiae, fructus arctii, rhizoma dioscoreae and probiotics. The compound traditional Chinese medicine preparation is scientifically matched according to the theory of syndrome differentiation and treatment of traditional Chinese medicine, has the effects of reducing blood sugar, clearing away heat and toxic materials, nourishing yin and tonifying kidney and weakness of spleen and lung, enhancesthe function of a human body, is high in cure rate, short in treatment course, low in recurrence rate and less in toxic and side effects, and has a better effect on relieving diabetes and complications caused by diabetes. The defects of pure traditional Chinese medicine treatment and western medicine treatment are overcome, and the traditional Chinese medicine preparation is worthy of clinical application and popularization.
Owner:DALIAN NATIONALITIES UNIVERSITY

Gold (III) complex-perylene diimide derivative, and fluorescent sensing tube and tubular fluorescent sensor prepared from same

The invention provides a gold (III) complex-perylene diimide derivative, and a fluorescent sensing tube and a tubular fluorescent sensor prepared from the same. A flexible rotatable cyclometalated alkynyl group (III) structure with good chemical stability and planar structure characteristics is introduced into a bending position modified perylene diimide anhydride position; the non-planarity of the formed structure effectively inhibits the strong intermolecular accumulation effect of perylene diimide, avoids aggregation-induced fluorescence quenching, and improves the luminous efficiency of perylene diimide; and the cyclometalated Au (III) complex and perylene diimide can also form an ideal energy donor-acceptor pair, so that the apparent Stokes shift of the system is effectively widened, and a foundation is laid for structural optimization of a sensor to be built. The gold (III) complex-perylene diimide derivative has good fluorescence performance, and the tubular fluorescence sensor is high in sensitivity.
Owner:SHAANXI NORMAL UNIV

Compound microorganism bacterium agent fertilizer for regulating and improving vegetable microenvironment and preparation method thereof

The invention relates to a compound microorganism bacterium agent fertilizer for regulating and improving a vegetable microenvironment. The compound microorganism bacterium agent fertilizer for regulating and improving the vegetable microenvironment is prepared from the following raw materials in parts by weight: 650 to 750 kg of molasses fermentation liquid, 280 to 290 kg of amino acid liquid, 2500 to 3500 g of lactobacillus plantarum, 1500 to 2500 g of lactobacillus acidophilus, 1000 to 2000 g of lactobacillus rhamnosus, 1000 to 2000 g of bifidobacterium, 1000 to 2000 g of lactobacillus casei, 1000 to 2000 g of chelated ferrous iron, 300 to 700 g of chelated zinc, 1000 to 2000 g of chelated copper, and 800 to 1200 g of chelated boron. The invention provides a preparation method of the compound microorganism bacterium agent fertilizer at the same time. According to the compound microorganism bacterium agent fertilizer for regulating and improving the vegetable microenvironment and thepreparation method thereof provided by the invention, a soil microenvironment for vegetable growth is changed fundamentally, and favorable soil is provided for plant growth.
Owner:SHANDONG GOOD BROS FERTILIZER CO LTD

Constructing composite nanomedicine by coupling drug components with nanoparticles, preparation method and application

The invention discloses a composite nano-drug constructed by nanoparticles coupled with pharmaceutical ingredients as well as a preparation method and an application of the composite nano-drug, and belongs to the technical field of composite drugs. The method for preparing the nano-drug comprises the following operations: modifying amino on the surfaces of nanoparticles which contain hydroxy in the surfaces, and activating biological or small-molecular drugs containing hydroxy; and then conducting a conjugate coupling reaction on the nanoparticles and the drugs, so that a plurality of chemicalbonds are constructed, combining the plurality of chemical bonds so as to prepare the nano-drug. Based upon researches, it is indicated that an anti-cancer effect can be greatly improved on the basisof a synergistic effect between active ingredients of natural drugs (such as taxol, ginsenoside and other traditional Chinese medicines) and a nano-material, and meanwhile, key and breakthrough points can be also provided for researching and producing efficient anti-cancer nano-drugs which are low or free from toxic and side effects and have targeting and autonomous targeting performances.
Owner:郑州天兆医疗科技有限公司

Cultivation method for wedge plowing and transplanting rice

The invention discloses a cultivation method for wedge plowing and transplanting rice, belonging to the field of rice cultivation. To solve the problem of the low yield of high-yield high-quality varieties in the exiting cultivation mode, the cultivation method of the invention comprises the following steps: (1) plowing soil; not carrying out plowing and watering after harvesting former crop in rotation; and digging a plurality of ditches on a surface of a farmland, wherein the row spacing between two adjacent ditches is 20cm, the ditch is narrow at the bottom and wide at the top and has an inverted triangular shape, the depth of the ditch is 5-10cm, the width of the ditch is 3-6cm, the two side faces of the ditch are all oblique planes relative to a horizontal plane, and the included angle alpha is 40-70 degrees between the oblique plane and the horizontal plane; (2) applying base fertilizer, transplanting seedlings, and watering; and (3) subsequently managing the field. The cultivation method of the invention reduces the farming intensity by using wedge plowing instead of traditional ploughing, applies fertilizer to the bottom of the wedge-type ditch in a centralized way, reduces the disturbance to the soil, is energy-saving and labor-saving, has large water storage capacity of the soil and high soil system stability, increases the utilization ratio of fertilizer, improves the growth environment of root system, and realizes the effects of saving cost and increasing yield and efficiency.
Owner:HUNAN AGRICULTURAL UNIV

A kind of high-efficiency planting method of a little red

The invention provides a high-efficiency planting method of a little red, which relates to the technical field of cultivation of Chinese medicinal materials. The high-efficient planting method of a little red of the present invention comprises the following steps: (1) selecting seeds, (2) preparing a germination accelerator, (3) Preparation of ecological mulch, (4) ridging, (5) seed pretreatment, (6) sowing and (7) field management; the high-efficiency planting method of a little red of the present invention has a high germination rate and a fast germination speed, which avoids to the greatest extent Manual weeding greatly saves production costs, improves the economic benefits of a little red planting, is good for the environment, and has no pollution, so it is worth promoting.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI

Method for inducing human umbilical cord derived mesenchymal stem cells to differentiate into chondrocytes and application thereof

PendingCN110205286AMaintain phenotypic stabilityBig contactCulture processSkeletal/connective tissue cellsMicrosphereCell surface membrane
A method for inducing human umbilical cord derived mesenchymal stem cells to differentiate into chondrocytes is characterized by including the steps: S1, resuscitating UC-MSCs, collecting the resuscitated UC-MSCs and preparing cell microspheres by a 3D pendant-drop method; and S2, replacing a culture medium for culturing the cell microspheres with a cartilage-induced differentiation culture medium, replacing the culture medium once every other 3 days, and culturing for 14-28 days. The method has the advantages: 1, in the preparation method, the UC-MSCs are prepared in a three-dimensional three-dimensional environment, so the contact between cells is broader and cell bridging and close connection and formation of cholangiole are facilitated, so as to maintain the UC-MSCs phenotype stability; and 2, 3D pendant-drop culture is adopted, so that cell arrangement is closer, changes in cell micro-environments makes a series of signal transduction and intracellular signal transduction betweenstem cell surface membrane molecules changed, so as to promote the differentiation of various stem cells.
Owner:WUHAN HAMILTON BIOTECH

Cultivation method for rice direct dibble seeding in wedged ditches

The invention discloses a cultivation method for rice direct dibble seeding in wedged ditches, which belongs to the field of rice cultivation. In order to solve the problem that yield potential of high-yield high-quality varieties is difficult to fulfill by existing cultivation methods, the cultivation method for rice direct dibble seeding in wedged ditches includes the steps: firstly, tilling soil, namely performing no turning or irrigation after preceding harvesting, and digging a field to obtain a plurality of ditches, wherein row spacing between each two adjacent ditches is 18-22cm, each ditch in a triangular shape is wide end up, each ditch is 5-20cm deep and 3-6cm wide, two sides of each ditch are slopes relative to the horizontal plane, and an included angle between each slope and the horizontal plane is 40 DEG-70 DEG; secondly, post-treating rice seeds; thirdly, performing base fertilizer application, direct dibble seeding, and irrigation; and fourthly, performing field management. The traditional ploughing is replaced with wedging, so that farming intensity is reduced, interference to soil is lowered, energy sources are saved, labor is reduced, seeds for use are few, soilwater retention capacity is high, soil system stable is high, fertilizer utilization rate is high, root system growth environment is improved, and cost reducing, yield increase and efficiency enhancement are achieved.
Owner:HUNAN AGRICULTURAL UNIV

A kind of gold (ⅲ) complex-perylene diimide derivative and its prepared fluorescence sensor tube and tube type fluorescence sensor

The invention provides a gold(III) complex-perylene diimide derivative and a fluorescence sensing tube and tube-type fluorescence sensor prepared therefrom. The alkyne-based (III) structure is introduced into the perylene diimide anhydride site modified by the curved position, and the non-planarity of the formed structure effectively suppresses the strong intermolecular stacking effect of the perylene diimide and avoids aggregation-induced fluorescence quenching. The cyclometalated Au(III) complex and the perylene diimide can also form an ideal energy donor-acceptor pair, thereby effectively broadening the surface of the system. The Stokes displacement is observed, which lays the foundation for the structural optimization of the sensor to be built. The gold(III) complex-perylene diimide derivative has good fluorescence properties, and the tubular fluorescence sensor has high sensitivity.
Owner:SHAANXI NORMAL UNIV
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