A kind of cell affinity agent and preparation method thereof for improving in vitro infection efficiency of duck flavin virus

An in vitro infection, duck flavivirus technology, applied in virus/phage, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of low cell infection efficiency, insufficient virus titer, slow primary virus culture process, etc. problem, to achieve the effect of improving in vitro infection efficiency and increasing affinity
CN104611298BActive Publication Date: 2017-03-22GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
Publication Date
2017-03-22

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Abstract

The invention discloses a cell affinity agent capable of improving duck flavivirus in-vitro infection efficiency and a preparation method of the cell affinity agent and belongs to the technical field of agents for microbial virus culture. The cell affinity agent is prepared from the following components in percentage by mass: 0.5-5% of organic acid, 0-1% of phosphoric acid, 0-3.5% of inorganic salt and the balance of deionized water. By the cell affinity agent, the microenvironment between the duck flavivirus and the cell can be improved, and the affinity between the duck flavivirus and the cell is increased so that the infection efficiency of the duck flavivirus to the cell is effectively increased.
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Description

technical field

[0001] The invention relates to the technical field of microbial viruses, in particular to a cell affinity agent for improving the infection efficiency of duck yellow virus in vitro and a preparation method thereof. Background technique

[0002] Duck yellow virus (duck Tembusu virus) is a virus that can seriously reduce the egg production of laying ducks. Its clinical manifestations are a sudden drop in egg production rate, a shortened peak egg production period, and severe egg production within a short period of time. The feed rate can be reduced to zero, and the feed intake will also decrease at the same time, and some of them will die. Pathological dissection showed serious lesions such as ovarian hyperemia, swelling, necrosis, and atrophy. Since the disease was first discovered in China in 2010, it has caused a large-scale reduction in the production of egg ducks in Shandong, Hunan, Guangdong and other major duck breeding provinces. At present, many res...

Claims

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