Cell affinity agent capable of improving duck flavivirus in-vitro infection efficiency and preparation method of cell affinity agent

A technology for in vitro infection and duck yellow virus, applied in the direction of virus/bacteriophage, microbe-based methods, biochemical equipment and methods, etc., can solve the problems of low cell infection efficiency, insufficient virus titer, slow primary virus culture process, etc. problems, to achieve the effect of improving in vitro infection efficiency and increasing affinity

Active Publication Date: 2015-05-13
GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many literature reports pointed out that the clinically isolated duck yellow virus can infect a variety of cells in vitro, such as VERO cells, BHK cells, etc., but the primary virus culture process is relatively slow, and the cell infection efficiency is low. There is still no obvious cell lesions, and the virus titer is not high enough, which is a problem that needs to be solved for the research and development of vaccines

Method used

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  • Cell affinity agent capable of improving duck flavivirus in-vitro infection efficiency and preparation method of cell affinity agent
  • Cell affinity agent capable of improving duck flavivirus in-vitro infection efficiency and preparation method of cell affinity agent
  • Cell affinity agent capable of improving duck flavivirus in-vitro infection efficiency and preparation method of cell affinity agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A kind of cell substantivity agent that improves duck yellow virus in vitro infection efficiency, it is prepared from the following components by mass percentage:

[0040]

[0041] Wherein, the inorganic salt is a mixture of sodium sulfate, disodium hydrogen phosphate, and potassium chloride.

[0042] Wherein, the sodium sulfate accounts for 0.3% of the total affinity agent; the sodium dihydrogen phosphate accounts for 1.6% of the total affinity agent; and the potassium chloride accounts for 1% of the total affinity agent.

[0043] Specifically, the affinity agent is prepared according to the following steps: weigh malonic acid, phosphoric acid, sorbic acid, malic acid, sodium sulfate, disodium hydrogen phosphate, potassium chloride according to the formula, Dissolve in deionized water according to formula and filter.

Embodiment 2

[0045] A kind of cell substantivity agent that improves duck yellow virus in vitro infection efficiency, it is prepared from the following components by mass percentage:

[0046]

[0047] Wherein, the inorganic salt is a mixture of sodium sulfate, disodium hydrogen phosphate, potassium chloride and sodium chloride.

[0048] Wherein, the sodium sulfate accounts for 0.25% of the total affinity agent; the sodium dihydrogen phosphate accounts for 1% of the total affinity agent; the potassium chloride accounts for 0.5% of the total affinity agent; The sodium chloride accounts for 0.75% of the total affinity agent.

[0049] Specifically, the affinity agent is prepared according to the following steps: weigh malonic acid, phosphoric acid, sorbic acid, malic acid, sodium sulfate, disodium hydrogen phosphate, potassium chloride, sodium chloride , Dissolve the above components in the deionized water of the formula, and filter to obtain.

Embodiment 3

[0051] A kind of cell substantivity agent that improves duck yellow virus in vitro infection efficiency, it is prepared from the following components by mass percentage:

[0052]

[0053]

[0054] Wherein, the inorganic salt is a mixture of sodium sulfate, disodium hydrogen phosphate, and sodium chloride.

[0055] Wherein, the sodium sulfate accounts for 0.2% of the total affinity agent; the sodium dihydrogen phosphate accounts for 1.5% of the total affinity agent; and the sodium chloride accounts for 1% of the total affinity agent.

[0056] Specifically, the affinity agent is prepared according to the following steps: weigh malonic acid, phosphoric acid, sorbic acid, malic acid, sodium sulfate, disodium hydrogen phosphate, sodium chloride according to the formula, Dissolve in deionized water according to formula and filter.

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Abstract

The invention discloses a cell affinity agent capable of improving duck flavivirus in-vitro infection efficiency and a preparation method of the cell affinity agent and belongs to the technical field of agents for microbial virus culture. The cell affinity agent is prepared from the following components in percentage by mass: 0.5-5% of organic acid, 0-1% of phosphoric acid, 0-3.5% of inorganic salt and the balance of deionized water. By the cell affinity agent, the microenvironment between the duck flavivirus and the cell can be improved, and the affinity between the duck flavivirus and the cell is increased so that the infection efficiency of the duck flavivirus to the cell is effectively increased.

Description

technical field [0001] The invention relates to the technical field of microbial viruses, in particular to a cell affinity agent for improving the infection efficiency of duck yellow virus in vitro and a preparation method thereof. Background technique [0002] Duck yellow virus (duck Tembusu virus) is a virus that can seriously reduce the egg production of laying ducks. Its clinical manifestations are a sudden drop in egg production rate, a shortened peak egg production period, and severe egg production within a short period of time. The feed rate can be reduced to zero, and the feed intake will also decrease at the same time, and some of them will die. Pathological dissection showed serious lesions such as ovarian hyperemia, swelling, necrosis, and atrophy. Since the disease was first discovered in China in 2010, it has caused a large-scale reduction in the production of egg ducks in Shandong, Hunan, Guangdong and other major duck breeding provinces. At present, many res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12R1/93
Inventor 冯晓声王贵平贾爱卿王伟
Owner GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
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