Method for inducing human umbilical cord derived mesenchymal stem cells to differentiate into chondrocytes and application thereof

A technology of mesenchymal stem cells and chondrocytes, applied in the field of biomedicine, can solve the problems that affect the clinical application of adipose MSCs, the quality of cells is affected by the donor, and the limited proliferation ability, so as to improve the potential of multidirectional differentiation, promote the ability of mineralization and differentiation, The effect of promoting stem cell differentiation
CN110205286APending Publication Date: 2019-09-06WUHAN HAMILTON BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN HAMILTON BIOTECH
Publication Date
2019-09-06

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Abstract

A method for inducing human umbilical cord derived mesenchymal stem cells to differentiate into chondrocytes is characterized by including the steps: S1, resuscitating UC-MSCs, collecting the resuscitated UC-MSCs and preparing cell microspheres by a 3D pendant-drop method; and S2, replacing a culture medium for culturing the cell microspheres with a cartilage-induced differentiation culture medium, replacing the culture medium once every other 3 days, and culturing for 14-28 days. The method has the advantages: 1, in the preparation method, the UC-MSCs are prepared in a three-dimensional three-dimensional environment, so the contact between cells is broader and cell bridging and close connection and formation of cholangiole are facilitated, so as to maintain the UC-MSCs phenotype stability; and 2, 3D pendant-drop culture is adopted, so that cell arrangement is closer, changes in cell micro-environments makes a series of signal transduction and intracellular signal transduction betweenstem cell surface membrane molecules changed, so as to promote the differentiation of various stem cells.
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Description

technical field

[0001] The invention relates to the field of biomedicine, regenerative medicine and bone disease technology, in particular to a method and application field for inducing human umbilical cord-derived mesenchymal stem cells to differentiate into chondrocytes. Background technique

[0002] Osteoarthritis (OA) is a chronic inflammatory disease of bones and joints, characterized by degeneration and destruction of articular cartilage, subchondral bone sclerosis and osteophyte formation, and inflammation and hyperplasia of surrounding soft tissues. joints, such as knee osteoarthritis (Knee osteoarthritis, KOA). There are about 1 billion OA patients in the world, of which about 100 million are Chinese patients. The incidence of OA increases with age. Preliminary estimates suggest that the prevalence of OA in my country is about 62% in people over 60 years old, and as high as 80% in people over 75 years old. The clinical manifestation is repeated and persistent bone...

Claims

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