Tube seedling slow growth conservation method for anoectochilus roxburghii germplasm

A preservation method and test-tube seedling technology, which are applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., to achieve the effects of lowering the cultivation temperature, reducing the light intensity, and reducing the number of generations.
CN109329048AInactive Publication Date: 2019-02-15谭兴江
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
谭兴江
Publication Date
2019-02-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a tube seedling slow growth conservation method for anoectochilus roxburghii germplasm. The method comprises the following steps: S1, material drawing and sterilization of explants; S2, inoculation and induction; S3, subculture multiplication; S4, low-temperature stress treatment of tube seedlings; S5, slow growth conservation of the tube seedlings; S6, rejuvenation of the tube seedlings; S7, rooting of the tube seedlings; S8, transplantation. The method effectively slows the growth speed of the tube seedlings by changing microenvironment conditions for the growth of germplasm materials, prolongs the subculture cycle, ensures that the germplasm preservation time reaches 3 to 4 years, is stable in inheritable character, high in germplasm survival rate and obvious in rejuvenation effect, and has important significance on further protection of the anoectochilus roxburghii resource and research, development and utilization of anoectochilus roxburghii.
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Description

technical field

[0001] The invention belongs to the biotechnology of medicinal plants in the field of agriculture and forestry, and in particular relates to a method for slow-growing and preserving test-tube seedlings of Aurea germplasm. Background technique

[0002] Clematis ( Anoectochilus roxburghii ) that is, Mosaic orchid, also known as golden silkworm, golden thread orchid, golden stone pine, golden thread tiger head banana, golden thread into the bone, etc., is a perennial herb of the genus Orchidaceae; its leaves are round and shield-shaped , the surface is dark brown, the underside is dark red, flannelette-like is obvious, and there are golden reticular veins called "golden thread". Clematis likes to be cool and humid, and especially likes to grow in ditch edges with evergreen broad-leaved trees, stone walls, and moist areas with loose soil. It is generally distributed in hilly lands at an altitude of 300 to 1,200 meters. my country is mostly distributed in Fujian...

Claims

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