A bright photobacterium and its application

A technology for bright photobacteria and uses, applied in the direction of bacteria, lyase, microorganisms, etc., can solve the problems of limited application, poor storage stability, and low production of alginate lyase, and achieve simple ingredients, simple preparation, and industrial scale-up production easy effect

Inactive Publication Date: 2016-01-20
ZHEJIANG SHUREN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the production of alginate lyase is low and the storage stability is poor, which greatly limits the application in industrial production.

Method used

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  • A bright photobacterium and its application
  • A bright photobacterium and its application

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Experimental program
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Effect test

Embodiment 1

[0036] A method for producing algin lyase, the specific method is as follows:

[0037] (1) The slant culture of Photobacterium luminescens SRU-2 was inoculated into a seed medium, and cultured at a temperature of 27.8°C and a shaker rotating speed of 180 rpm for 14-16 hours to the logarithmic growth phase to obtain a seed liquid. The basic formula is as follows: each 100mL seed culture medium contains tryptone 1.80g, yeast extract 0.48g, sodium chloride 2.80g, glycerol 0.80g, and the pH value is natural;

[0038] (2) Prepare the fermentation medium according to the ratio of 30 mL fermentation medium to 500 mL shake flask, transfer the seed liquid obtained in step (1) to the fermentation medium at a 2.0% inoculum, at a temperature of 27.8°C and pH value 6.6. Cultivate for 22~26h under the condition of a shaker rotation speed of 180rpm to obtain a fermentation broth. The formula of the fermentation medium is as follows: each 100mL fermentation medium contains 0.50g glucose, 0.18g pep...

Embodiment 2

[0042] A method for producing algin lyase, the specific method is as follows:

[0043] (1) The slant culture of Photobacterium luminescens SRU-2 was inoculated into a seed medium, and cultured at a temperature of 28.5°C and a shaker rotation speed of 220 rpm for 14-16 hours to the logarithmic growth phase to obtain a seed liquid. The basic formula is as follows: every 100mL seed culture medium contains 2.20g tryptone, 0.52g yeast extract, 3.20g sodium chloride, 1.20g glycerol, and the pH value is natural;

[0044] (2) Prepare the fermentation medium according to the ratio of 30 mL fermentation medium to 500 mL shake flask, transfer the seed liquid obtained in step (1) to the fermentation medium at a 2.0% inoculum, at a temperature of 28.5°C, pH value 7.6. Cultivate for 22~26h at 220 rpm of the shaker to obtain the fermentation broth. The formula of the fermentation medium is as follows: each 100mL fermentation medium contains 0.70g glucose, 0.25g beef extract, 0.52g sodium chloride...

Embodiment 3

[0048] A method for producing algin lyase, the specific method is as follows:

[0049] (1) The slant culture of Photobacterium luminescens SRU-2 was inoculated into a seed medium, and cultured at a temperature of 28.0°C and a shaker rotation speed of 200 rpm for 14-16 hours to the logarithmic growth phase to obtain a seed liquid. The basic formula is as follows: every 100mL seed culture medium contains tryptone 2.00g, yeast extract 0.50g, sodium chloride 3.00g, glycerol 1.00g, pH value is natural;

[0050] (2) Prepare the fermentation medium according to the ratio of 30 mL fermentation medium to 500 mL shake flask, transfer the seed liquid obtained in step (1) to the fermentation medium at a 2.0% inoculum, at a temperature of 28.0°C, pH value 8.6. Cultivate for 22~26h under the condition of a shaker speed of 200rpm to obtain a fermentation broth. The fermentation medium formula is as follows: each 100mL fermentation medium contains 0.85g glucose, 0.20g beef extract, 0.50g potassium...

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Abstract

The present invention relates to a microbial strain and its application, in particular to a luminous luminescent bacterium and its application, aiming to solve the problem that there is no report in the prior art on the use of luminous luminous bacterium to produce alginate lyase, and the production of alginate by other bacterial strains The production of lyase is low and the storage stability is poor, which greatly limits the application in industrial production. The Photobacterium luminosa SRU-2 was preserved in the China Center for Type Culture Collection, date of preservation: December 29, 2013, preservation number: CCTCCM? NO: M2013723. The bacterial strain can be used to produce alginate lyase. The present invention utilizes Photobacterium luminosa to produce alginate lyase with high yield and good stability of enzyme solution during storage, which greatly expands the application range of alginate lyase in industrial production. .

Description

technical field [0001] The invention relates to a microbial strain and its application, in particular to a bright photobacterium and its application. Background technique [0002] Alginate lyase (alginatelyase) specifically acts on 1→4 glycosidic bonds, and catalyzes the degradation of alginate through the β-elimination mechanism to produce uronic acid oligosaccharides containing unsaturated bonds and uronic acid monomers with unsaturated bonds. No hydrolase that can act on algin has been found. Therefore, algin lyase is currently the only effective algin degrading enzyme. According to its substrate specificity, algin lyase is divided into three categories. The first category can degrade manna Uronic acid (PM), called PM enzyme (EC4.2.2.3); the second type can degrade guluronic acid (PG), called PG enzyme (EC4.2.2.11); the third type is double A functional enzyme that can degrade both PM and PG. [0003] Alginate lyase is not only used as a tool enzyme for the preparation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/88C12R1/01
Inventor 刘彩琴陈蔚青蔡成岗王楠陈虹金建昌
Owner ZHEJIANG SHUREN UNIV
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